Skip to main content
Log in

The prophase stages in human foetal oocytes studied by light and electron microscopy

  • Original Investigations
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

The progression of the prophase stages of meiosis in human foetal ovaries is reported from a series of aborted foetuses spanning the first two trimesters of gestation. A surface-spreading technique allowed cells to be examined at both light and electron microscope levels. The pachytene stage was specifically examined for evidence of synaptic or other abnormalities. Two observations of interest are the relatively high incidence with which errors of pairing occur and second the state of thickening observed when such unpaired axes are stained with silver.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Baker TG (1963) A quantitative and cytological study of germ cells in human oocytes. Proc R Soc Lond [Biol] 158:417–433

    Google Scholar 

  • Baker TG, Franchi LL (1967) The fine structure of oogonia and oocytes in human ovaries. J Cell Sci 2:213–224

    Google Scholar 

  • Bojko M (1983) Human meiosis VIII. Chromosome pairing and formation of the synaptonemal complex in oocytes. Carlsberg Res Commun 48:457–483

    Google Scholar 

  • Burgoyne PS, Baker TG (to be published) Meiotic pairing and gametogenic failure. In: Controlling events in meiosis. Soc for Exp Biol Symp 38. Cambridge University Press, Cambridge

  • Chandley AC, Goetz P, Hargreave TB, Joseph AM, Speed RM (to be published) On the nature and extent of XY pairing at meiotic prophase in man. Cytogenet Cell Genet

  • Fletcher JM (1979) Light microscope analysis of meiotic prophase chromosomes by silver staining. Chromosoma 72:241–248

    Google Scholar 

  • Ford CE (1975) The time in development at which gross genome imbalance is expressed. In: Balls M, Wild AE (eds) The early development of mammals. Brit Soc for Dev Biol Symp 2. Cambridge University Press, Cambridge, pp 285–304

    Google Scholar 

  • Goetz P, Chandley AC, Speed RM (1984) Morphological and temporal sequence of meiotic prophase development at puberty in the male mouse. J Cell Sci 65:249–263

    Google Scholar 

  • Henderson SA, Edwards RG (1968) Chiasma frequency and maternal age in mammals. Nature 218:22–28

    Google Scholar 

  • Holm PB, Rasmussen SW (1983) Human meiosis V. Substages of pachytene in human spermatogenesis. Carlsberg Res Commun 48:351–383

    Google Scholar 

  • Jacobs PA, Hassold TJ (1980) The origin of chromosome abnormalities in spontaneous abortions. In: Porter IH, Hook EB (eds) Human embryonic and fetal death. Academic Press, New York, pp 289–298

    Google Scholar 

  • Kindred JF (1963) The chromosomes of the ovary of the human fetus. Anat Res 147:295–302

    Google Scholar 

  • Kofman-Alfaro S, Chandley AC (1970) Meiosis in the male mouse. An autoradiographic investigation. Chromosoma 31:404–420

    Google Scholar 

  • Kurilo LF (1981) Oogenesis in antenatal development in man. Hum Genet 57:86–92

    Google Scholar 

  • Luciani JM, Stahl A (1971) Etude des states de debut de la meiose chez l'ovocyte foetal humain. Bull Assoc Anat (Nancy) 15:445–458

    Google Scholar 

  • Manotaya T, Potter EL (1963) Oocytes in prophase of meiosis from squash preparations of human fetal ovaries. Fertil Steril 14:378–392

    Google Scholar 

  • Mikkelsen M, Poulsen H, Grinsted J, Lange A (1980) Non-disjunction in trisomy 21: study of chromosomal heteromorphisms in 110 families. Ann Hum Genet 44:17–28

    Google Scholar 

  • Monesi V (1965) Synthetic activities during spermatogenesis in the mouse. Exp Cell Res 39:197–224

    Google Scholar 

  • Moses MJ, Poorman PA (1981) Synaptonemal complex analysis of mouse chromosomal rearrangements. II. Synaptic adjustment in tandem duplications. Chromosoma 81:519–535

    Google Scholar 

  • Moses MJ, Counce SJ, Paulson DF (1975) Synaptonemal complex complements of man in spreads of spermatocytes with detail of the sex chromosome pair. Science 187:363–365

    Google Scholar 

  • Moses MJ, Poorman PA, Polani PA, Crolla JA, Moir F (1982) Synapsis, synaptic adjustment and DNA synthesis in meiotic prophase of mouse oocytes. J Cell Biol 95:77a

    Google Scholar 

  • Ohno S, Klinger HP, Atkin NB (1962) Human oögenesis. Cytogenetics 1:42–51

    Google Scholar 

  • Ohno S, Makino S, Kaplan WD, Kinosita R (1961) Female germ cells of man. Exp Cell Res 24:106–110

    Google Scholar 

  • Rasmussen SW, Holm PB (1980) Mechanics of meiosis. Hereditas 93:187–216

    Google Scholar 

  • Rodeck CH, Morsman JM, Gosden CM, Gosden JR (1983) Development of an improved technique for first-trimester microsampling of chorion. Br J Obstet Gynaecol 90:1113–1118

    Google Scholar 

  • Simoni G, Brambati B, Danesino C, Rossella F, Terzoli GL, Ferrari M, Fraccaro M (1983) Efficient direct chromosome analysis and enzyme determination from chorionic villi in the first trimester of pregnancy. Hum Genet 63:349–357

    Google Scholar 

  • Solari AJ (1980) Synaptonemal complexes and associated structures in microspread human spermatocytes. Chromosoma 81:315–337

    Google Scholar 

  • Speed RM (1982) Meiosis in the foetal ovary. I. An analysis at the light microscope level using surface-spreading. Chromosoma 85:427–437

    Google Scholar 

  • Speed RM (1984) Meiotic configurations in female trisomy 21 foetuses. Hum Genet 66:176–180

    Google Scholar 

  • Speed RM, Chandley AC (1983) Meiosis in the foetal ovary. II. Oocyte development and age-related aneuploidy. Does a production line exist? Chromosoma 88:184–189

    Google Scholar 

  • Sung WK, Jagiello G (1982) A technique for light and electron microscopy of the synaptonemal complex of the mouse oocyte. Can J Genet Cytol 24:675–680

    Google Scholar 

  • Sung WK, Komatsu M, Jagiello G (1983) A method for obtaining synaptonemal complexes of human pachytene oocytes. Caryologia 36:315–324

    Google Scholar 

  • Tres LL (1975) Nucleolar RNA synthesis of meiotic prophase spermatocytes in the human testis. Chromosoma 53:141–151

    Google Scholar 

  • Wallace BMN, Hulten MA (1983) Triple chromosome synapsis in oocytes from a human foetus with trisomy 21. Ann Hum Genet 47:271–276

    Google Scholar 

  • von Wettstein D (1977) The assembly of the synaptonemal complex. Philos Trans R Soc Lond [Biol] 277:235–243

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Speed, R.M. The prophase stages in human foetal oocytes studied by light and electron microscopy. Hum Genet 69, 69–75 (1985). https://doi.org/10.1007/BF00295532

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00295532

Keywords

Navigation