Abstract
The visualization of subcellular organelles and structures is a valuable tool for understanding cellular changes that occur in oocytes and early embryos as a result of genetic alterations, incubation conditions, drug treatments, and many other manipulations. Preparing oocytes for transmission electron microscopic analysis can be challenging as these cells cannot be visualized without a microscope and they are more susceptible to mechanical disruption during manipulation. Here we describe methods for immobilizing oocytes on either a solid surface or within a matrix and then document our embedding techniques which work well for preserving the ultrastructure of the mouse oocyte.
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Acknowledgement
This work was supported by NICHD grant HD38353-07A2 to S.A.C.
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Anguish, L., Coonrod, S. (2013). Preparation of Mammalian Oocytes for Transmission Electron Microscopy. In: Homer, H. (eds) Mammalian Oocyte Regulation. Methods in Molecular Biology, vol 957. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-191-2_15
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DOI: https://doi.org/10.1007/978-1-62703-191-2_15
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-190-5
Online ISBN: 978-1-62703-191-2
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