Abstract
Two experiments were conducted to evaluate FD and C Red Dye #3 for its developmental toxicity and psychotoxicity. Adult Sprague-Dawley rats were fed diets containing the dye for 2 weeks and were then bred. The diets were continued for the females throughout gestation and lactation and were provided continuously to their offspring thereafter. The treatment groups for Experiment 1 were Red Dye #3 as 0.0, 0.25, 0.5, or 1.0% of the diet (w/w), and a positive control group treated with the toxin hydroxyurea on days 2–10 of life (50 mg/kg/day, s.c.); Experiment 2 was a replication of Experiment 1 with the same dose groups, but without the positive control group. Parental animals were evaluated for weight and food consumption, and females for reproductive success. The offspring were assessed on a series of tests using the Cincinnati Psychoteratogenicity Screening Test Battery, plus weight, food consumption, physical landmarks of development, and brain weight. Red-3 produced no reductions in parental or offspring weight or food consumption. Red-3 significantly increased preweaning offspring mortality in the first experiment, but not in the second. Behaviorally, Red-3 produced no dose-dependent effects that replicated across the two experiments. It was concluded that no evidence was obtained that dietary exposure to FD and C Red Dye #3 (erythrosine) is psycho toxic to developing rats.
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Supported in part by USFDA project 223-75-2030
Now at the Western Behavioral Sciences Institute, La Jolla, California, USA
Now at the Medical Foundation of Buffalo, Buffalo, New York, USA
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Vorhees, C.V., Butcher, R.E., Brunner, R.L. et al. A developmental toxicity and psychotoxicity evaluation of FD and C Red Dye #3 (erythrosine) in rats. Arch Toxicol 53, 253–264 (1983). https://doi.org/10.1007/BF00294991
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DOI: https://doi.org/10.1007/BF00294991