Summary
The effects of the dihydropyridine-type calcium antagonist (nitrendipine) and agonist (Bay K 8644) in comparison to atropine have been studied after intravenous administration on spontaneous and pelvic-nerve-induced contraction of rat urinary bladder. Bay K 8644 increased the basal internal bladder pressure as well as the amplitude of the spontaneous bladder contractions in a dose-dependent manner. In addition, an increase in systemic arterial blood pressure was noted for a period of about 20 min. In the presence of atropine the effects of Bay K 8644 on the urinary bladder were almost completely antagonized. Both nitrendipine and atropine reduced in a dose-dependent manner the amplitude of spontaneous and nerve-induced bladder contraction. The spontaneous and nerve-induced bladder contractions were significantly reduced by atropine or nitrendipine. Only nitrendipine caused a reduction of the spontaneous bladder contraction frequency. The systemic blood pressure was decreased significantly by nitrendipine but not after atropine administration. We suggest that both calcium antagonist and agonist can change the tension of the urinary bladder in vivo. As a side-effect the systemic blood pressure is altered. Atropine can antagonize the effect of BayK 8644 on the urinary bladder and reduces spontaneous and nerve-induced bladder contractions more specifically than nitrendipine.
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References
Brading A (1987) Physiology of bladder smooth muscle: In: Torrens M, Morrison JFB (eds) The physiology of lower urinary tract. Springer, Berlin Heidelberg New York, p 161
Caine M (1984) Non-autonomic drugs acting on the urinary tract. In: Caine M (ed) The pharmacology of the urinary tract. Springer, Berlin Heidelberg New York, p 31
Carpenter FG, Rubin RM (1967) The motor innervation of the rat urinary bladder. J Physiol (Land) 192:609
Documenta Geigy (1968) In: Diem D, Lentner C (eds) Wissenschaftliche Tabellen, 7th edn. Geigy, Basel
Fleckenstein A (1985) Calcium antagonists and calcium agonists: fundamental criteria and classification. In: Eleckenstein A, Breemen C van, Groß R, Hoffmeister F (eds) Cardiovascular effects of dihydropyridine-type calcium antagonists and agonists. Springer, Berlin Heidelberg New York, p 3
Fovaeus M, Andersson KE, Batra S, Morgan E, Sjögren C (1987) Effects of calcium, calcium channel blockers and Bay K 8644 on contractions induced by muscarinic receptor stimulation of isolated bladder muscle from rabbit and man. J Urol 137:798
Hertle L, Nawrath H (1989) Stimulation of voltage-dependent contractions by calcium channel activator Bay K 8644 in the human upper urinary tract. J Urol 141:1014
Lacovou JW, Hill SJ, Birmingham AT (1990) Agonist-induced contraction and accumulation of inositol phosphates in the guinea-pig detrusor: evidence that muscarinic and purinergic receptors raise intracellular calcium by different mechanisms. J Urol 144:775
Maggi CA, Grimaldi G, Meli A (1982) The effects of nifedipine and verapamil on spontaneous and carbachol-stimulated contractions of rat urinary bladder “in vivo”. Arch Int Pharmacodyn 257:288
Maggi CA, Manzini S, Parlani M, Conte B, Giuliani S, Meli A (1988) The effect of nifedipine on spontaneous, drug-induced and reflexly-activated contractions of the rat urinary bladder: evidence for the participation of an intracellular calcium store to micturition contraction. Gen Pharmacol 19:73
Maggi CA, Giuliani S, Patacchini R, Turini D, Barbanti G, Giachetti A, Meli A (1989) Multiple sources of calcium for contraction of the human urinary bladder muscle. Br J Pharmacol 98:1021
Malmgren A, Andersson KE, Andersson PO, Fovaeus M, Sjögren C (1988) Effects of nifedipine and Bay K 8644 on normal and hypertrophied rat detrusor. Neurourol Urodyn 7:27
Mikkelsen E, Kazda S, Nyborg NCB (1985) Effects of light and Bay K 8644, a new 1,4-dihydropyridine, on mechanical response of rat thoracic aorta. Acta Pharmacol Toxicol 56:126
Sato S, Hayashi H, Garfield RE (1989) Mechanical response of the rat uterus, cervix, and bladder to stimulation of hypogastric and pelvic nerves in vivo. Biol Reprod 40:209
Schramm M, Thomas G, Towart R, Frankowiak G (1983) Novel dihydropyridines with positive inotropic action through activation of Ca2+ channels. Nature 303:535
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Diederichs, W., Sroka, J. & Graff, J. Comparison of Bay K 8644, nitrendipine and atropine on spontaneous and pelvic-nerve-induced bladder contractions on rat bladder in vivo. Urol. Res. 20, 49–53 (1992). https://doi.org/10.1007/BF00294335
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DOI: https://doi.org/10.1007/BF00294335