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Expression of small heat-shock protein hsp 27 in reactive gliosis in Alzheimer disease and other types of dementia

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Abstract

Immunohistochemical and immunoblotting analysis of brain tissue of Alzheimer's disease (AD) patients showed highly induced expression of the small heat-shock protein hsp 27 in affected cortex. Expression of hsp 27 was present in a large number of proliferating astrocytes. The highest expression was exhibited by degenerative astrocytes in the areas rich in senile plaques. Neurofibrillary tangles, Hirano bodies and some hippocampal neurons were also positive. Expression of hsp 27 increased with the severity of AD-specific morphological changes, and with the duration of dementia. In control brains immunoreaction was restricted to the vessels and to occasional astrocytes in the white matter. Similar patterns of immunoreactivity were present in cases without dementia (Parkinson disease, lacunar state, or focal ischemic necrosis). Patients suffering from other types of dementia (Parkinson/dementia complex, multi-infarct dementia, normal pressure hydrocephalus) showed less expression of hsp 27 in reactive astrocytes than AD, but more than controls. These results indicate that increased expression of hsp 27, especially in astrocytes showing klazmatodendrosis, is associated with AD pathology.

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References

  1. Aoyama A, Fröhli E, Schafer R, Klemenz R (1993) AlphaB-Crystallin expression in mouse NIH 3T3 fibroblasts: glucocorticoid responsiveness and involvement in thermal protection. Mol Cell Biol 13:1824–1835

    Google Scholar 

  2. Cissé S, Perry G, Lacoste-Royal G, Cabana T, Gauvreau D (1993) Immunochemical identification of ubiquitin and heatshock proteins in corpora amylacea from normal aged and Alzheimer's disease brains. Acta Neuropathol 85:233–240

    Google Scholar 

  3. Craig EA (1993) Chaperones: helpers along the pathways to protein folding. Science 260:1902–1903

    Google Scholar 

  4. Crete P, Landry J (1990) Induction of hsp 27 phosphorylation and thermoresistance in Chinese hamster cells by arsenite, cycloheximide, A23187 and EGTA. Radiat Res 121: 320–327

    Google Scholar 

  5. Delacourte A (1990) General and dramatic glial reaction in Alzheimer brains. Neurology 40: 33–37

    Google Scholar 

  6. Eddleston M, Mucke L (1993) Molecular profile of reactive astrocytes: implications for their role in neurological disease. Neuroscience 54: 15–36

    Google Scholar 

  7. Engel K, Knauf U, Gaestel M (1991) Generation of antibodies against human hsp 27 and murine hsp 25 by immunization with a chimeric small heat shock protein. Biomed Biochim Acta 50:1065–1071

    Google Scholar 

  8. Frederickson RCA (1992) Astroglia in Alzheimer's disease. Neurobiol Aging 13:239–253

    Google Scholar 

  9. Gernold M, Knauf U, Gaestel M, Stahl J, Kloetzel P-M (1993) Development and tissue-specific distribution of mouse small heat shock protein hsp 25. Dev Genet 14:103–111

    Google Scholar 

  10. Giulian D, Vaca K, Corpuz M (1993) Brain glia release factors with opposing actions upon neuronal survival. J Neurosci 13: 29–37

    Google Scholar 

  11. Harrison PJ, Procter AW, Exworthy T, Roberts GW, Najlerahim A, Barton AJL, Pearson RCA (1993) Heat shock protein (hsx 70) mRNA expression in human brain: effects of neurodegenative disease and agonal state. Neuropathol Appl Neurobiol 19:10–21

    Google Scholar 

  12. Iwaki T, Wisniewski T, Iwaki A, Corbin E, Tomokane N, Tateishi J, Goldman JE (1992) Accumulation of αB-crystallin in central nervous system glia and neurons in pathological conditions. Am J Pathol 140: 345–356

    Google Scholar 

  13. Iwaki T, Iwaki A, Tateishi J, Sakaki Y Goldman JE (1993) Alpha-B-Crystallin and 27-kDa heat shock protein are regulated by stress conditions in the central neryous system and accumulate in Rosenthal fibers. Am J Pathol 143: 487–495

    Google Scholar 

  14. Jaenicke R, Creighton TE (1993) Junior chaperones. Curr Biol 3:234–235

    Google Scholar 

  15. Jakob U, Gaestel M, Engel K, Buchner J (1993) Small heat shock proteins are molecular chaperones. J Biol Chem 268: 1517–1520

    Google Scholar 

  16. Kato M, Herz F, Kato S, Hirano A (1992) Expression of stress-response (heat-shock) protein 27 in human brain tumors: an immunohistochemical study. Acta Neuropathol 83: 420–422

    Google Scholar 

  17. Kato M, Brijlall D, Adler SA, Kato S, Herz F (1992) Effect of hyperosmolality on alkaline phosphatase and stress-response protein 27 of MCF-7 breast cancer cells. Breast Cancer Res Treat 23:241–249

    Google Scholar 

  18. Kato S, Hirano A, Umahara T, Kato M, Herz F, Ohama E (1992) Comparative immunohistochemical study on the expression of αB crystallin, ubiquitin and stress-response protein 27 in ballooned neurons in various disorders. Neuropathol Appl Neurobiol 18: 335–340

    Google Scholar 

  19. Kato K, Shinohara H, Goto S, Inaguma Y, Morishita R, Asano T (1992) Copurification of small heat shock protein with αB crystallin from human skeletal muscle. J Biol Chem 11: 7718–7725

    Google Scholar 

  20. Kato K, Goto S, Hasegawa K, Shinohara H, Inaguma Y (1993) Response to heat shock of αB crystallin and HSP28 in U373 MG human glioma cells. Biochim Biophys Acta 1175: 257–262

    Google Scholar 

  21. Khachaturian ZS (1985) Diagnosis of Alzheimer's disease. Arch Neurol 42: 1097–1104

    Google Scholar 

  22. Klemenz R, Andres AC, Fröhli E, Schäfer R, Aoyama A (1993) Expression of the murine small heat shock proteins hsp 25 and αB crystallin in the absence of stress. J Cell Biol 120: 639–645

    Google Scholar 

  23. Knauf U, Bielka H, Gaestel M (1992) Over-expression of the small heat-shock protein, hsp 25, inhibits growth of Ehrlich ascites tumor cells. FEBS Lett 309: 297–302

    Google Scholar 

  24. Landry J, Chretien P, Lambert H, Hickey E, Weber LA (1989) Heat shock resistance confered by expression of the human HSP27 gene in rodent cells. J Cell Biol 109: 7–15

    Google Scholar 

  25. Lavoie JN, Gingras-Breton G, Tanguay RM, Landry J (1993) Induction of chinese hamster HSP27 gene expression in mouse cells confers resistance to heat shock. J Biol Chem 15: 3420–3429

    Google Scholar 

  26. Mantrope M, Rudge JS, Varon S (1986) Astroglial cell contributions to neuronal survival and neuritic growth. In: Fedoroff S, Vernadakis A (eds) Astrocytes. Academic Press, New York, pp 315–376

    Google Scholar 

  27. Merek KB, Groenen PJTA, Voorter CEM, de Hard-Hoekman WA, Horwitz J, Bloemendal H, de Jong WW (1993) Structural and fuctional similarities of bovine α-crystalline and mouse small heat-shock protein. A family of chaperones. J Biol Chem 268: 1046–1052

    Google Scholar 

  28. Nowak T (1993) Synthesis of heat shock/stress proteins during cellular injury. Ann NY Acad Sci 679: 142–156

    Google Scholar 

  29. Renkawek K, de Jong WW, Merck KB, Frenken CWGM, van Workum FPA, Bosman GJCGM (1992) αB-crystallin is present in reactive glia in Creutzfeldt-Jakob disease. Acta Neuropathol 83: 324–327

    Google Scholar 

  30. Renkawek K, Voorter CEM, Bosman GJCGM, van Workum FPA, de Jong WW (1994) Expression of αB-crystallin in Alzheimer disease. Acta Neuropathol 87: 155–160

    Google Scholar 

  31. Renkawek K, Bosman GJCGM, Gaestel M (1993) Increased expression of heat-shock protein 27 kDa in Alzheimer disease: a preliminary study. NeuroReport 5: 14–16

    Google Scholar 

  32. Tanaguay RM, Wu Y, Khandjian EW (1993) Tissue-specific expression of heat shock proteins of the mouse in the absence of stress. Dev Genet 14: 112–118

    Google Scholar 

  33. Welch WJ (1992) Mammalian stress response-cell physiology, structure/function of stress proteins, and implications for medicine and disease. Physiol Rev 72: 1063–1081

    Google Scholar 

  34. Yokoyama N, Iwaki T, Goldman JE, Tateishi J, Fukui M (1993) Small heat-shock protein is expressed in meningiomas and in granulofilamentous inclusion bodies. Acta Neuropathol 85: 248–255

    Google Scholar 

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Authors and Affiliations

  1. Institute of Neurology, University Hospital Nijmegen, P.O. Box 9101, NL-6500, Nijmegen, The Netherlands

    K. Renkawek

  2. Department of Biochemistry, University Hospital Nijmegen, P.O. Box 9101, NL-6500 HB, Nijmegen, The Netherlands

    G. I. C. G. M. Bosman & W. W. de Jong

Authors
  1. K. Renkawek
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  2. G. I. C. G. M. Bosman
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  3. W. W. de Jong
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Supported by a fellowship of the Royal Netherlands Academy of Arts and Sciences

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Renkawek, K., Bosman, G.I.C.G.M. & de Jong, W.W. Expression of small heat-shock protein hsp 27 in reactive gliosis in Alzheimer disease and other types of dementia. Acta Neuropathol 87, 511–519 (1994). https://doi.org/10.1007/BF00294178

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  • DOI: https://doi.org/10.1007/BF00294178

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