Skip to main content
SpringerLink
Log in

Improved assay of neurotoxic esterase for screening organophosphates for delayed neurotoxicity potential

  • Original Investigations
  • Published:
Archives of Toxicology Aims and scope Submit manuscript

Abstract

The assay of neurotoxic esterase (NTE) in brains taken from dosed hens enables potential neurotoxicity of organophosphate pesticides, plasticers, etc. to be assessed. The original assay [Johnson, M. K. Biochem. J. 114, 711–717 (1969)] has been simplified to eliminate centrifugation and transfer steps and both the selectivity and the sensitivity have been increased. The procedures necessary to obtain stable reagent stocks are described.

Zusammenfassung

Durch Bestimmung der neurotoxischen Esterase (NTE) ist es möglich, im Gehirn von mit phosphororganischen Pflanzenschutzmitteln, Weichmachern und anderen Stoffen behandelten Hühnern die potentielle Neurotoxizität dieser Stoffe zu erfassen. Die ursprüngliche Methode [Johnson, M. K. Biochem. J. 114, 711–717 (1969)] wurde vereinfacht, so daß Zentrifugieren und Transferschritte nicht mehr erforderlich sind. Die Selektivität und Empfindlichkeit der Methode wurde verbessert. Die Herstellung stabiler Reagentienstammlösungen wird beschrieben.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Johnson, M. K.: The delayed neurotoxic effect of some organophosphorus compounds: identification of the phosphorylation site as an esterase. Biochem. J. 114, 711–717 (1969)

    Google Scholar 

  • Johnson, M. K.: Organophosphorus esters causing delayed neurotoxic effects: mechanism of action and structure/activity relationships. Arch. Toxicol. 34, 259–288 (1975a)

    Google Scholar 

  • Johnson, M. K.: The delayed neuropathy caused by some organophosphorus esters: mechanism and challenge. Crit. Rev. Toxicol. 3, 289–316 (1975b)

    Google Scholar 

  • Johnson, M. K.: The initial biochemical events leading to the delayed neuropathy caused by some organophosphorus esters. In: The prediction of chronic toxicity from short term studies, (W. A. M. Duncan, B. J. Leonard, M. Brunaud, Eds.). Proc. Eur. Soc. Toxicol. Vol. 17, pp. 7–12. Amsterdam: Excerpta Medica 1976

    Google Scholar 

  • Pound, D. W., Hartley, G. S., Heath, D. F., Hulme, J. M.: Improvements in or relating to phosphoruscontaining organic compounds. Brit. Pat. 688, 787 (1953)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Molecular Toxicology Section, MRC Toxicology Unit, Medical Research Council Laboratories, Woodmansterne Road, SM5 4EF, Carshalton, Surrey, UK

    M. K. Johnson

Authors
  1. M. K. Johnson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Johnson, M.K. Improved assay of neurotoxic esterase for screening organophosphates for delayed neurotoxicity potential. Arch Toxicol 37, 113–115 (1977). https://doi.org/10.1007/BF00293860

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00293860

Key words

Search

Navigation