Summary
A cDNA probe corresponding to mRNA encoding a closely related variant of human insulin-like growth factor II (IGF-II) was used for the chromosomal assignment of the IGF-II gene. Southern blot hybridization analysis of DNA from human-Chinese hamster somatic cell hybrids showed that the human IGF-II gene is located on chromosome 11. Using the same IGF-II variant probe, a cosmid was isolated which contains the human IGF-II gene. Restriction enzyme analysis revealed that the gene encoding IGF-II has a discontinuous structure and contains at least four exons.
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Bartram CR, De Klein A, Hagemeijer A, Van Agthoven T, Geurts van Kessel A, Bootsma D, Grosveld G, Ferguson-Smith MA, Davies T, Stone M, Heisterkamp N, Stephenson JR, Groffen J (1983) Translocation of c-abl oncogene correlates with the presence of a Philadelphia chromosome in chronic myelocytic leukemia. Nature 306:277–280
Bell GI, Merryweather JP, Sanchez-Pescador R, Stempien MM, Priestly L, Scott J, Rall LB (1984) Sequence of a cDNA clone encoding human preproinsulin-like growth factor II. Nature 310: 775–777
Brissenden JE, Ullrich A, Francke U (1984) Human chromosomal mapping of genes for insulin-like growth factors I and II and epidermal growth factor. Nature 310:781–784
Clemmons DR, Van Wyk JJ (1981) Somatomedin-C and platelet derived growth factor stimulate human fibroblast replication. J Cell Physiol 106:361–367
Daughaday WH (1977) Hormonal regulation of growth by somatomedin and other tissue growth factors. Clin Endocrinol Metab 6:117–135
Dull TJ, Gray A, Hayflick JS, Ullrich A (1984) Insulin-like growth factor II precursor gene organization in relation to insulin gene family. Nature 310:777–781
Geurts van Kessel AHM, Ten Brinke H, Boere WAM, Den Boer WC, De Groot PG, Hagemeijer A, Meera Khan P, Pearson PL (1981) Characterization of the Philadelphia chromosome by gene mapping. Cytogenet Cell Genet 30:83–91
Harris H, Watkins JF (1965) Hybrid cells derived from mouse and man: artificial heterokaryons of mammalian cells from different species. Nature 205:640–646
Höppener JWM, de Pagter-Holthuizen P, Geurts van Kessel AHM, Jansen M, Lips CJM, Sussenbach JS (1985) The human gene encoding insulin-like growth factor I is located on chromosome 12. (To be published)
Ish-Horowicz D, Burke JF (1981) Rapid and efficient cosmid cloning. Nucleic Acids Res 9:2989–2998
Jansen M, Van Schaik FMA, Ricker AT, Bullock B, Woods DE, Gabbay KH, Nussbaum AL, Sussenbach JS, Van den Brande JL (1983) Sequence of cDNA encoding human insulin-like growth factor I precursor. Nature 306:609–611
Jansen M, van Schaik FMA, van Tol H, Van den Brande JL, Sussenbach JS (1985) Nucleotide sequences of cDNAs encoding precursors of human insulin-like growth factor II (IGF-II) and an IGF-II variant. FEBS Lett 179:243–246
Klapper DG, Svoboda ME, Van Wyk JJ (1983) Sequence analysis of somatomedin-C: confirmation of identity with insulin-like growth factor I. Endocrinology 112:2215–2217
Lebo RV, Kan YW, Cheun MC, Carranco AV, Ju LC, Chang JC, Cordell B, Goodman HM (1982) Assigning the polymorphic human insulin gene to the short arm of chromosome 11 by chromosome sorting. Hum Genet 60:10–15
Littlefield JW (1964) Selection of hybrids from mating of fibroblasts in vitro and their presumed recombinants. Science 145:709–710
Marquardt H, Todaro GJ, Henderson LE, Oroszlan S (1981) Purification and primary structure of a polypeptide with multiplication stimulation activity from rat liver cell cultures. J Biol Chem 256: 6859–6865
Rinderknecht E, Humbel RE (1978a) Primary structure of human insulin-like growth factor II. FEBS Lett 89:283–286
Rinderknecht E, Humbel RE (1978b) The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin. J Biol Chem 253:2769–2776
Schoenle E, Zapf J, Humbel RE, Froesch ER (1982) Insulin-like growth factor I stimulates growth in hypophysectomized rats. Nature 296:252–253
Tricoli JV, Rall LB, Scott J, Bell GI, Shaws TB (1984) Localization of insulin-like growth factor genes to human chromosomes 11 and 12. Nature 310:784–786
van Buul-Offers SC, Van den Brande JL (1979) Effect of growth hormone and peptide fractions containing somatomedin activity on growth and cartilage metabolism of Snell dwarf mice. Acta Endocrinol (Copenh) 92:242–257
Van den Brande JL, Hoogerbrugge C (1980) Plasma fractions containing somatomedin activity. Part I: Extraction and partial characterization. In: Pecile A, Müller EE (eds) Growth hormone and other biologically active peptides. Amsterdam, Excerpta Medica, pp 81–102
van Ommen GJB, Arnberg AC, Baas F, Brocas H, Sterk A, Tegelaars WHH, Vassart G, de Vylder JJM (1983) The human thyroglobulin gene contains two 15–17 kb introns near its 3′-end. Nucleic Acids Res 11:2273–2285
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de Pagter-Holthuizen, P., Höppener, J.W.M., Jansen, M. et al. Chromosomal localization and preliminary characterization of the human gene encoding insulin-like growth factor II. Hum Genet 69, 170–173 (1985). https://doi.org/10.1007/BF00293291
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DOI: https://doi.org/10.1007/BF00293291