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In vivo P-31 NMR measurements of phosphate metabolism in Platymonas subcordiformis as related to external pH

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Abstract

The phosphate metabolism of Platymonas subcordiformis was investigated by 31P-NMR spectroscopy with special attention on the effect of external pH. Glycolyzing cells and cells energized by respiration or photosynthesis gave spectra dependent upon their metabolic state. The transition from deenergized to energized states is accompanied by a shift of cytoplasmic pH from 7.1–7.4, an increase of ATP level and-in well energized cells-the appearance of a new signal tentatively assigned to phosphoarginine.

The spectra remain stable over a wide range of external pH. Cytoplasmic pH is well regulated in respiring cells for external pH in the range 5.3–12.3. The typical 0.4 units difference of internal pH in energized as compared to deenergized cells is not affected by external pH in the range 6–12. The intensity of a signal attributed to PEP is markedly increased at high external pH. pH regulation is less efficient below external pH of 6 in deenergized cells. Below pH 3.8 oxidative phosphorylation ceases. Upon raising cytoplasmic pH to 7.4 in deenergized cells polyphosphate chains start to disintegrate.

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Abbreviations

PEP:

Phosphoenolpyruyate

P i :

inorganic phosphate

PP i :

inorganic pyrophosphate

poly P:

polyphosphates

PP-1, PP-2, PP-3:

terminal, second, and third phosphate residue of polyphosphates

PP-4:

core phosphate residues of polyphosphates

pH i , pH o :

internal (cytoplasmic) and external pH

NTP/NDP:

nucleotide triphosphate/-diphosphate

S/N:

signal to noise ratio

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Authors and Affiliations

  1. Fachbereich 1-Physik, Universität Bremen, D-2800, Bremen 33, Federal Republic of Germany

    H. Kugel & A. Mayer

  2. Fachbereich 2-Biologie, Universität Bremen, D-2800, Bremen 33, Federal Republic of Germany

    G. O. Kirst

  3. Fachbereich 2-Chemie, Universität Bremen, D-2800, Bremen 33, Federal Republic of Germany

    D. Leibfritz

Authors
  1. H. Kugel
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  2. A. Mayer
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  3. G. O. Kirst
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  4. D. Leibfritz
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Kugel, H., Mayer, A., Kirst, G.O. et al. In vivo P-31 NMR measurements of phosphate metabolism in Platymonas subcordiformis as related to external pH. Eur Biophys J 14, 461–470 (1987). https://doi.org/10.1007/BF00293255

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  • DOI: https://doi.org/10.1007/BF00293255

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