Summary
In four rice (Oryza sativa L.) mutants resistant to hydroxy-L-proline (Hyp), HYP101, HYP203, HYP205 and HYP210, and in their original variety, Nipponbare, free proline and Hyp contents in the seeds and in the 14-day-old seedlings have been determined. The four mutants can be divided into two groups: HYP101 and HYP203 are classified as to recessive gene and the levels of free proline are similar to that of the original variety; the second group includes mutants HYP205 and HYP210 where the Hyp resistance is transmitted heterozygously and, both in the seeds and in the seedlings, a remarkable increase in free proline content is observed. In particular, free proline contents in the seeds of HYP205 and HYP210 are, respectively, 24 and 12 times that of the original variety. Hyp is detected only in the seedlings cultured with Hyp solution. In the Hyp resistant seedlings of HYP205 and HYP210, Hyp contents are twice that of the original variety and less than half in the seedlings of HYP101 and HYP203. Hyp resistance and differential proline levels are also evident in the callus initiated from the mutants. This suggests that the Hyp resistant mutants are good genetic markers both in planta and in vitro. The Hyp mutants are also discussed with regard to stress resistance.
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References
Aspinall D, Paleg LG (1981) Proline accumulation: Physiological aspects. In: Paleg LG, Aspinall D (eds) The physiology and biochemistry of drought resistance in plants. Academic Press, Sydney, pp 205–241
Bright SWJ, Featherstone LC, Miflin BJ (1979a) Lysine metabolism in a barley mutant resistant to S-(2-aminoethyl) cysteine. Planta 146:629–633
Bright SWJ, Norbury PB, Miflin BJ (1979b) Isolation of a recessive barley mutant resistant to S-(2-aminoethyl)L cysteine. Theor Appl Genet 55:1–4
Bright SWJ, Kueh JSH, Franklin J, Rognes ER, Miflin BJ (1982) Two genes for threonine accumulation in barley seeds. Nature 299:278–279
Duncan DR, Widholm JM (1987) Proline accumulation and its implication in cold tolerance of regenerable maize callus. Plant Physiol 83:703–708
Hasegawa H (1988) Relationship between lysine plus threonine resistance and threonine overproduction in rice (Oryza sativa L.) seedlings. Jpn J Breed 38:10–16
Hasegawa H, Inoue M (1983) Induction and selection of hydroxy-L-proline resistant mutant in rice (Oryza sativa L.). Jpn J Breed 33:275–282
Hasegawa H, Mori S (1986) Non-proline-accumulating rice mutants resistant to hydroxy-L-proline. Theor Appl Genet 72:226–230
Hasegawa H, Mori S, Inoue M, Murakami M (1985) HydroxyL-proline resistant mutants in rice. Rice Genet Newslett 2:88–89
James T, Jacobs M (1976) A p-fluorophenylalanine resistant mutant in Arabidopsis thaliana. In: Kranz AR (ed) Arabidopsis information service, Proc 2nd Int Symp Arabidopsis Res. J. W. Goethe Univ, Frankfurt, pp 85–95
Kueh JSH, Bright SWJ (1981) Proline accumulation in a barley mutant resistant to trans-4-hydroxy-L-proline. Planta 153:166–171
Kueh JSH, Bright SWJ (1982) Biochemical and genetical analysis of three proline-accumulating barley mutants. Plant Sci Lett 27:233–341
Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18:100–127
Miflin BJ, Bright SWJ, Rognes SE, Kueh JSH (1983) Amino acids, nutrition and stress: the role of biochemical mutants in solving problems of crop quality. In: Kosuge T, Meredith CP, Hollaender A (eds) Genetic engineering of plants — an agricultural perspective. Plenum Press, London, pp 391–414
Mori S, Hasegawa H, Nakamura K, Nakanishi H, Murakami M (1985) Characterization of callus-cell lines of rice resistant to hydroxy-L-proline. Rice Genet Newslett 2:89–91
Mori S, Hasegawa H, Nakanishi H, Murakami M (1986) Characterization of hydroxy-L-proline resistant mutants in rice. In: Proc 6th Int Congr Plant Tissue Cell Culture. Univ Minnesota, Minneapolis, pp 380
Negrutiu I, Cattoir-Reynearts A, Verbruggen I, Jacobs M (1984) Lysine overproducer mutants with an altered dihydrodipicolinate synthase from protoplast culture of Nicotiana sylvestris (Spegazzini and Comes). Theor Appl Genet 68:11–20
Pandey R, Ganapathy PS (1985) The proline enigma: NaCl-tolerant and NaCl-sensitive callus lines of Cicer arietinum. Plant Sci 40:13–17
Ricardi G, Cella R, Camerino G, Ciferri O (1983) Resistance to azetidine-2-carboxylic acid and sodium chloride tolerance in carrot cell cultures and Spirulina platensis. Plant Cell Physiol 24:1073–1078
Stewart GR, Larher F (1980) Accumulation of amino acid and related compounds in relation to environmental stress. In: Miflin BJ (ed). The biochemistry of plants, a comprehensive treatise. vol V. Academic Press, London, pp 609–635
Van Swaaij AC, Jacobsen E, Kiel JAKW, Feenstra WJ (1986) Selection, characterization and regeneration of hydroxyproline resistant cell lines of Solanum tuberosum: Tolerance to NaCl and freezing stress. Physiol Plant 68:359–366
Van Swaaij AC, Nijdam H, Jacobsen E, Feenstra WJ (1987) Increased frost tolerance and amino acid content in leaves, tubers and leaf callus of regenerated hydroxyproline resistant potato clones. Euphytica 36:369–380
Wakasa K, Widholm JM (1987) A 5-methyltryptophan resistant rice mutant, MTR1, selected in tissue culture. Theor Appl Genet 74:49–54
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Mori, S., Hasegawa, H., Che, R. et al. Free proline contents in two different groups of rice mutants resistant to hydroxy-L-proline. Theoret. Appl. Genetics 77, 44–48 (1989). https://doi.org/10.1007/BF00292314
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DOI: https://doi.org/10.1007/BF00292314