Summary
The cytosine analogue 5-azacytidine induces very distinct undercondensations in human chromosomes if applied to lymphocyte cultures. The number of induced undercondensations and their chromosomal localization can be varied by the 5-azacytidine dose and the treatment time. “Pulverized” chromosomes or undercondensations in the G-band-positive chromosome regions are produced with high doses and long treatment times. If applied in low doses during the last hours of culture, 5-azacytidine induces specific undercondensations in the heterochromatin of chromosomes 1, 9, 15, 16, and Y. Optimum conditions required for inducing the various types of undercondensation in the chromosomes were determined. Various examples of the use 5-azacytidine in the analysis of chromosome rearrangements involving heterochromatic regions are presented.
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References
Gosden JR, Mitchel AR, Buckland RA, Clayton RP, Evans HJ (1975) The location of four human satellite DNAs on human chromosomes. Exp Cell Res 92:148–158
Hanks SK, Gollin SM, Rao PN, Wray W, Hittelman WN (1983) Cell cycle-specific changes in the ultrastructural organization of prematurely condensed chromosomes. Chromosoma 88:333–342
Jones KW, Prosser J, Corneo G, Ginelli E, Bobrow M (1973) Satellite DNA, constitutive heterochromatin, and human evolution. In: Pfeiffer RA (ed) Modern aspects of cytogenetics: constitutive heterochromatin in man. Schattauer, New York, pp 45–61
Marcus M, Goitein R, Gropp A (1979) Condensation of all human chromosomes in phase G2 inhibited by 33258-Hoechst treatment. Hum Genet 51:99–105
Miklos GLG, John B (1979) Heterochromatin and satellite DNA in man: properties and prospects. Am J Hum Genet 31:264–280
Miller OJ, Schnedl W, Allen J, Erlanger BF (1974) 5-Methylcytosine localised in mammalian constitutive heterochromatin. Nature 251:636–637
Pfeiffer RA (1974) Cell cultures from blood and bone marrow. In: Schwarzacher HG, Wolf U (eds) Methods in human cytogenetics. Springer, Berlin Heidelberg New York, pp 1–37
Razavi L (1965) An inexpensive and simple method for preparing chromosome spreads. Proc Soc Exp Biol Med 118:717–719
Schmid M, Schempp W, Olert J (1982) Comparative analysis of karyotypes in European shrew species. II. Constitutive heterochromatin, replication patterns and sister chromatid exchanges in Sorex araneus and S. gemellus. Cytogenet Cell Genet 34:124–135
Schmid M, Grunert D, Haaf T, Engel W (1983) A direct demonstration of somatically paired heterochromatin of human chromosomes. Cytogenet Cell Genet 36:554–561
Schnedl W, Dev VG, Tantravahi R, Miller DA, Erlanger BF, Miller OJ (1975) 5-Methylcytosine in heterochromatic regions of chromosomes: chimpanzee and gorilla compared to the human. Chromosoma 52:59–66
Schweizer D, Ambros P, Andrle M (1978) Modification of DAPI banding on human chromosomes by prestaining with a DNA binding oligopeptide antibiotic, distamycin A. Exp Cell Res 111:327–332
Sumner AT (1972) A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res 75:304–306
Viegas-Péquignot E, Dutrillaux B (1976) Segmentation of human chromosomes induced by 5-ACR (5-azacytidine). Hum Genet 34:247–254
Viegas-Péquignot E, Dutrillaux B (1981) Detection of G-C rich heterochromatin by 5-azacytidine in mammals. Hum Genet 57:134–137
Zadražil S, Fučik W, Bartl P, Šormová Z, Šorm F (1965) The structure of DNA from Escherichia coli cultures in the presence of 5-azacytidine. Biochim Biophys Acta 108:701–703
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Schmid, M., Haaf, T. & Grunert, D. 5-Azacytidine-induced undercondensations in human chromosomes. Hum Genet 67, 257–263 (1984). https://doi.org/10.1007/BF00291352
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DOI: https://doi.org/10.1007/BF00291352