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TFS1: A suppressor of cdc25 mutations in Saccharomyces cerevisiae

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Summary

The TFS1 gene of Saccharomyces cerevisiae is a dosage-dependent suppressor of cdc25 mutations. Overexpression of TFS1 does not alleviate defects of temperature-sensitive adenylyl cyclase (cdc35) or ras2 disruption mutations. The ability of TFS1 to suppress cdc25 is allele specific: the temperature-sensitive cdc25-1 mutation is suppressed efficiently but the cdc25-5 mutation and two disruption mutations are only partially suppressed. TFS1 maps to a previously undefined locus on chromosome XII between RDN1 and CDC42. The DNA sequence of TFS1 contains a single long open reading frame encoding a 219 amino acid polypeptide that is similar in sequence to two mammalian brain proteins. Insertion and deletion mutations in TFS1 are haploviable, indicating that TFS1 is not essential for growth.

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Authors and Affiliations

  1. Laboratory of Molecular Biology, University of Wisconsin, 1525 Linden Dr., 53706, Madison, WI, USA

    Lucy C. Robinson

  2. Department of Microbiology, North Carolina State University, Box 7615, 27695, Raleigh, NC, USA

    Kelly Tatchell

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  1. Lucy C. Robinson
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  2. Kelly Tatchell
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Robinson, L.C., Tatchell, K. TFS1: A suppressor of cdc25 mutations in Saccharomyces cerevisiae . Molec. Gen. Genet. 230, 241–250 (1991). https://doi.org/10.1007/BF00290674

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  • DOI: https://doi.org/10.1007/BF00290674

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