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Inheritance and phenotype expression of functional and null alleles of aromatic alcohol dehydrogenase (CAD) in diploid wheats

  • Plant Genetics
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Abstract

Functional F and null 0 alleles of the CAD1 (Aadh1) gene, which controls the biosynthesis of aromatic alcohol dehydrogenase, were studied in hybrids of the diploid wheat T. monococcum L. and Triticum sinskajae A. Filat. et Kurk. The gene CAD1 is located in chromosome 5A and is linked with the awnless gene awnS (La) with a recombination frequency of about 32%. Plants with genotypes FF, F0, and 00 were significantly different in the height and mechanical strength of the stalk (culm). The elastic limit of the culm tissues of plants FF was considerably higher than in 00 plants. F0 heterozygotes had intermediate values. The thickness of the wall of the sclerenchyma was thinner in plants with genotype 00. The chemical structure of lignin of plants with the functional CAD allele contained units of a phloroglucinol series missing in the mutant plants. The CAD genotypes had no effect on the relative content of cellulose and lignin in stalks of diploid wheat and insignificantly influenced the ratio of H: G: S units in the lignin structure, as well as some components of extractives. IR-spectroscopy found differences in the distribution of components of cell walls and extractives on the outer and inner surfaces of the culm. The results are discussed in relation to the applied aspects of the use of herbal products. Samples of diploid wheat with various genotypes of CAD can be used as model objects in breeding and genetic research.

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Authors and Affiliations

  1. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    A. A. Konovalov & N. P. Goncharov

  2. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia

    I. K. Shundrina, E. V. Karpova & A. A. Nefedov

Authors
  1. A. A. Konovalov
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  2. I. K. Shundrina
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  3. E. V. Karpova
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  4. A. A. Nefedov
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  5. N. P. Goncharov
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Correspondence to A. A. Konovalov.

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Original Russian Text © A.A. Konovalov, I.K. Shundrina, E.V. Karpova, A.A. Nefedov, N.P. Goncharov, 2014, published in Genetika, 2014, Vol. 50, No. 11, pp. 1310–1318.

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Konovalov, A.A., Shundrina, I.K., Karpova, E.V. et al. Inheritance and phenotype expression of functional and null alleles of aromatic alcohol dehydrogenase (CAD) in diploid wheats. Russ J Genet 50, 1161–1168 (2014). https://doi.org/10.1134/S1022795414110052

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  • DOI: https://doi.org/10.1134/S1022795414110052

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