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Cytogenetic analysis of the X chromosome region 2B3-4 — 2B11 of Drosophila melanogaster

IV. Mutation at the swi (singed wings) locus interfering with the late 20-OH ecdysone puff system

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Abstract

Mutation t467, belonging to the swi complementation group, and causing death in late prepupa, is located in the interval from 2B6 to the left part of 2B7-8. In this region puffing is absent in salivary gland chromosomes. In t467/t467 homozygotes intermoult early and early-late larval 20-OH ecdysone puffs do not differ from the controls. Mid-prepupal puffs are normal too with a few exceptions. However, all late larval and prepupal puffs are reduced or absent in the mutant. Both, hormone incubation of t467 glands in vitro and hormone injection have shown: i) 20-OH ecdysone in vitro does not restore the normal larval puffing pattern. ii) Withdrawal of the hormone from glands at PS6 causes premature appearance of late larval puffs, which, however, do not reach control sizes. It is concluded that the swi gene product is necessary for induction of late puffs. Thus in the 2B3-4—2B7-8 region three genes, affecting 20-OH ecdysone induction processes, have become known.

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Authors and Affiliations

  1. Group of Drosophila Cytogenetics, Institute of Cytology and Genetics, Siberian Division of U.S.S.R. Academy of Sciences, 630090, Novosibirsk, USSR

    Elena S. Belyaeva & Igor F. Zhimulev

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  1. Elena S. Belyaeva
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  2. Igor F. Zhimulev
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Belyaeva, E.S., Zhimulev, I.F. Cytogenetic analysis of the X chromosome region 2B3-4 — 2B11 of Drosophila melanogaster . Chromosoma 86, 251–263 (1982). https://doi.org/10.1007/BF00288680

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