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Cytogenetic analysis of chromosome region 89A of Drosophila melanogaster: isolation of deficiencies and mapping of Po, Aldox-1 and transposon insertions

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Summary

We have initiated a cytogenetic analysis of chromosome region 89A of Drosophila melanogaster by isolating a set of radiation-induced mutations causing loss of function of P[(w)B]1-1, a transposon bearing the white locus inserted in 89A. Complementation tests and cytological examination of these chromosomes identified four new deficiencies (Df(3R)Po 2, Df(3R)Po 3, Df(3R)Po 4 and Df(3R)c(3)G 2 ). The new deficiencies and three previously identified deficiencies (Df(3R)sbd 26, Df(3R)sbd 45 and Df(3R)sbd 105) were tested for the ability to complement mutations in the enzyme loci Po and Aldox-1, the indirect flight muscle genes Tm2 and act88F, the morphological mutations jvl, sbd 2 and Sb, the vital loci srp, pnr and mor, and a newly described vital locus l(3)89Aa. We also used linkage analysis to determine the order and relative positions of P[(w)B]1-1 and an independent transposon insertion, P[w+]21, with respect to cv-c, Po, Aldox-1 and sbd 2. Cytological examination of the deficiencies and analysis of the transformed lines by in situ hybridization permits the correlation of genetically defined regions with specific polytene chromosome bands. A revised cytogenetic map of the 8817–8913 region is presented.

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  1. Catherine R. Nelson & Paul Szauter

    Present address: Department of Biological Sciences, University of Nevada, Las Vegas, 4505 Maryland Parkway, 89154-4004, Las Vegas, NV, USA

Authors and Affiliations

  1. Department of Biological Chemistry, The Milton S. Hershey Medical Center, Pennsylvania State University, 17033, Hershey, Pennsylvania, USA

    Catherine R. Nelson & Paul Szauter

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  1. Catherine R. Nelson
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  2. Paul Szauter
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Communicated by B.H. Judd

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Nelson, C.R., Szauter, P. Cytogenetic analysis of chromosome region 89A of Drosophila melanogaster: isolation of deficiencies and mapping of Po, Aldox-1 and transposon insertions. Molec. Gen. Genet. 235, 11–21 (1992). https://doi.org/10.1007/BF00286176

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  • DOI: https://doi.org/10.1007/BF00286176

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