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Cytogenetic analysis of the 2B3-4-2B11 Region of the X-chromosome of Drosophila melanogaster

I. Cytology of the region and mutant complementation groups

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Abstract

There are, at least, 11 distinct single bands and one real doublet in the region 2B1-2-2C1-2 of the X-chromosome of Drosophila melanogaster. This figure coincides with that in Bridges' revised map with most of the “doublet” bands being artifacts. Three puffs appear in the region. The early ecdysone-specific puff 2B5-6, small at PS(puffing stage) 1, increases sharply at PS 2. The late ecdysone-specific puff 2C1-2 appears at PS 4–5. At PS 9–10, when 2B5-6 disappears completely and 2C1-2 decreases, a third puff at 2B11 appears. None of these puffs is active at PS 11. Morphological analysis of puff appearance and autoradiographic study of 3H-uridine incorporation into chromosomes carrying rearrangements within the 2B region suggest that the early ecdysone-specific puff derives from bands 2B5 and may be 2B6, while the neighbouring bands 2B1-4, and 2B7-10 do not show appreciable transcription at the investigated stages.

There are 42 mutations affecting viability in the region where the 2B5-6 puff is located. The mutations belong to 6 complementation groups; two of the groups dor and swi, are independent while the rest are overlapped by several lethal mutations (overlapping complex.) Mutants of the different groups have series of similar characteristics: temperature sensitivity, dose sensitivity, larva-pupal lethality and similar morphological abnormalities. It can be assumed that there is a functionally linked cluster of genes within the region 2B. Complementation groups br, rbp, l(1)pp-, l(1)pp-2 (overlapping complex) have been located by rearrangements in very narrow cytological limit 2B3-4-2B5 that is in the area of developing puff. Two other loci dor and swi are situated some to the right of 2B5.

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Authors and Affiliations

  1. Siberian Branch, Institute of Cytology and Genetics of the Academy of Sciences of U.S.S.R., 630090, Novosibirsk, U.S.S.R.

    E. S. Belyaeva, M. G. Aizenzon, V. F. Semeshin, E. M. Baritcheva, T. D. Gorelova & I. F. Zhimulev

  2. Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary

    I. I. Kiss & K. Koczka

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  1. E. S. Belyaeva
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  2. M. G. Aizenzon
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  3. V. F. Semeshin
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  4. I. I. Kiss
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  5. K. Koczka
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  6. E. M. Baritcheva
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  7. T. D. Gorelova
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  8. I. F. Zhimulev
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Belyaeva, E.S., Aizenzon, M.G., Semeshin, V.F. et al. Cytogenetic analysis of the 2B3-4-2B11 Region of the X-chromosome of Drosophila melanogaster . Chromosoma 81, 281–306 (1980). https://doi.org/10.1007/BF00285954

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