Abstract
The chromosomes of an established cell line of Dipodomys panamintinus have been characterised in terms of their C, G and Q banding patterns, and the distribution of silver grains in autoradiographs of chromosomes labelled in early or late S phase. No relationship could be established between C, G or Q banding regions of chromosomes and a particular S phase time of replication of the DNA in these banded regions. The implication of this result to the concept of heterochromatin is discussed.
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Arrighi, F. E., Hsu, T. C., Pathak, S., Sawada, H.: The sex chromosomes of the Chinese hamster: constitutive heterochromatin deficient in repetitive DNA sequences. Cytogenet. Cell Genet. 13, 268–274 (1974)
Bostock, C. J., Christie, S.: Quinacrine fluorescence staining of chromosomes and its relationship to DNA base composition. Exp. Cell Res. 86, 157–161 (1974a)
Bostock, C. J., Christie, S.: Chromosome banding and DNA replication studies on a cell line of Dipodomys merriami. Chromosoma (Berl.) 48, 73–87 (1974b)
Bostock, C. J., Prescott, D. M.: Buoyant density of DNA synthesised at different stages of the S phase of mouse L cells. Exp. Cell Res. 64, 267–274 (1971)
Bostock, C. J., Prescott, D. M., Hatch, F. T.: Timing of replication of the satellite and main band DNAs in cells of the kangaroo rat (Dipodomys ordii). Exp. Cell Res. 74, 487–495 (1972)
Brown, S. W.: Heterochromatin. Science 151, 417–425 (1966)
Buckland, R. A., Evans, H. J., Sumner, A. T.: Identifying mouse chromosomes with the ASG technique. Exp. Cell Res. 69, 231–236 (1971)
Caspersson, T., Farber, S., Foley, G. E., Kudynowski, J., Modest, E. J., Simonsson, E., Wagh, U., Zech, L.: Chemical differentiation along metaphase chromosomes. Exp. Cell Res. 49, 219–222 (1968)
Comings, D. E.: The structure of human chromosomes. In: The cell nucleus (H. Busch, ed.), vol. I, p. 538–564. New York-London: Academic Press 1974
Comings, D. E., Avelino, E., Okada, T. A., Wyandt, A. E.: The mechanism of C and G banding of chromosomes. Exp. Cell Res. 77, 469–493 (1973)
Comings, D. E., Mattochia, E.: DNA of mammalian and avian heterochromatin. Exp. Cell Res. 71, 113–131 (1972)
Deaven, L. L., Petersen, D. F.: The chromosomes of CHO, an aneuploid Chinese hamster cell line: G-band, C-band, and autoradiographic analyses. Chromosoma (Berl.) 41, 129–144 (1973)
Ellison, J. R., Barr, H. J.: Quinacrine fluorescence of specific chromosome regions. Late replication and high A:T content in Samoaia leonensis. Chromosoma (Berl.) 36, 375–390 (1972)
Evans, H. J.: Uptake of 3H-thymidine and patterns of DNA replication in nuclei and chromosomes of Vicia faba. Exp. Cell Res. 35, 381–393 (1964)
Evans, H. J., Buckton, K. E., Sumner, A. T.: Cytological mapping of human chromosomes: results obtained with qninacrine fluorescence and the acetic-saline-Giemsa techniques. Chromosoma (Berl.) 35, 310–325 (1971)
Evans, H. J., Buckland, R. A., Sumner, A. T.: Chromosome homology and heterochromatin in goat, sheep and ox studied by banding techniques. Chromosoma (Berl.) 42, 383–402 (1973)
Flamm, W. G., Bernheim, N. J., Brubaker, P. E.: Density gradient analysis of newly replicated DNA from synchronised mouse lymphoma cells. Exp. Cell Res. 64, 97–104 (1971)
Ganner, E., Evans, H. J.: The relationship between patterns of DNA replication and of quinacrine fluorescence in the human chromosome complement. Chromosoma (Berl.) 35, 326–341 (1971)
Golomb, H. M., Bahr, G. F.: Correlation of the fluorescent banding pattern and ultrastructure of a human chromosome. Exp. Cell Res. 84, 121–126 (1974)
Heitz, E.: Des Heterochromatin der Moose. I. Jb. wiss. Bot. 69, 762–818 (1928)
Hsu, T. C.: Heterochromatin pattern in metaphase chromosomes of Drosophila melanogaster. J. Hered. 62, 285–287 (1971)
Hsu, T. C.: Longitudinal differentiation of chromosomes. Ann. Rev. Genet. 7, 153–176 (1973)
Hsu, T. C., Arrighi, F. E.: Distribution of constitutive heterochromatin in mammalian chromosomes. Chromosoma (Berl.) 34, 243–253 (1971)
Kato, H., Moriwaki, K.: Factors involved in the production of banded structures in mammalian chromosomes. Chromosoma (Berl.) 38, 105–120 (1972)
Kato, H., Yoshida, T. H.: Banding patterns of Chinese hamster chromosomes revealed by new techniques. Chromosoma (Berl.) 36, 272–280 (1972)
Levine, E. M.: Mycoplasma contamination of animal cell cultures: a simple rapid detection method. Exp. Cell Res. 74, 99–109 (1972)
Levine, E. M., Thomas, L., McGregor, D., Hayflick, L., Eagle, H.: Altered nucleic acid metabolism in human cell cultures infected with Mycoplasma. Proc. nat. Acad. Sci. (Wash.) 60, 583–589 (1968)
Lima de Faria, A., Jaworska, H.: Late DNA synthesis in heterochromatin. Nature (Lond.) 217, 138–142 (1968)
Lubs, H. A., McKenzie, W. H., Patil, S. R., Merrick, S.: New staining methods for chromosomes. In: Methods in cell biology (D. M. Prescott, ed.), vol. VI, p. 345–380. New York-London: Academic Press 1973
Mace, M. L., Tevethia, S. S., Brinkley, B. R.: Differential immunofluorescent labelling of chromosomes with antisera specific for single stranded DNA. Exp. Cell Res. 75, 521–523 (1972)
Mazrimas, J. A., Hatch, F. T.: A possible relationship between satellite DNA and the evolution of kangaroo rat species (genus Dipodomys). Nature (Lond.) New Biol. 240, 102–105 (1972)
McKay, R. D. G.: The mechanism of G and C banding in mammalian metaphase chromosomes. Chromosoma (Berl.) 44, 1–14 (1973)
Miller, O. J., Schreck, R. R., Beiser, S. M., Erlanger, B. F.: Immunofluorescent studies of chromosome banding with antinucleoside antibodies. In: Chromosome identification — techniques and applications in biology and medicine. (T. Caspersson and L. Zech, eds.), p. 43–48. New York-London: Academic Press 1973
Pachmann, U., Rigler, R.: Quantum yield of acridines interacting with DNA of defined base sequence. Exp. Cell Res. 72, 602–608 (1972)
Pardue, M. L., Gall, J. G.: Chromosomal localisation of mouse satellite DNA. Science 168, 1356–1358 (1970)
Pathak, S., Arrighi, F. E.: Loss of DNA following C-banding procedures. Cytogenet. Cell Genet. 12, 414–422 (1973)
Pathak, S., Hsu, T. C., Utakoji, T.: Relationships between patterns of chromosome banding and DNA synthetic sequences: a study on the chromosomes of the Seba's fruit bat, Carollia perspicillata. Cytogenet. Cell Genet. 12, 157–164 (1973)
Prescott, D. M., Bostock, C. J., Hatch, F. T., Mazrimas, J. A.: Location of satellite DNAs in the chromosomes of the kangaroo rat (Dipodomys ordii). Chromosoma (Berl.) 42, 205–213 (1973)
Schmid, W.: Heterochromatin in mammals. Arch. Klaus-Stift. Vererb.-Forsch. 42, 1–60 (1967)
Sharma, T., Dhaliwal, M. K.: Relationship between patterns of late S DNA synthesis and C- and G-banding muntjak chromosomes. Exp. Cell Res. 87, 394–397 (1974)
Stock, A. D.: Chromosome evolution in the genus Dipodomys and its phylogenetic implications. Mammalian Chromosome Newsletter 12, 122–128 (1971)
Sumner, A. T., Evans, H. J.: Mechanisms involved in the banding of chromosomes with quinacrine and Giemsa. II. The interaction of the dyes with the chromosomal components. Exp. Cell Res. 81, 223–236 (1973)
Tobia, A., Schildkraut, C. L., Maio, J. J.: DNA replication in synchronised cultured mammalian cells. I. Time of synthesis of molecules of different average guanine and cytosine content. J. molec. Biol. 54, 499–515 (1970)
Vosa, C. G.: Heterochromatin recognition with fluorochromes. Chromosoma (Berl.) 30, 366–372 (1970)
Weisblum, B., Haseth, P. L. de: Quinacrine, a chromosome stain specific for deoxyadenylate-deoxythymidylate-rich regions in DNA. Proc. nat. Acad. Sci. (Wash.) 69, 629–632 (1972)
Yunis, J. J., Yasmineh, W. G.: Model for mammalian constitutive heterochromatin. Advanc. Cell molec. Biol. 1, 1–46 (1972)
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Bostock, C.J., Christie, S. Chromosomes of a cell line of Dipodomys panamintinus (kangaroo rat) a banding and autoradiographic study. Chromosoma 51, 25–34 (1975). https://doi.org/10.1007/BF00285804
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DOI: https://doi.org/10.1007/BF00285804