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Expression of the Phytophthora infestans ipiB and ipi0 genes in planta and in vitro

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Abstract

The ipiB and ipiO genes of the potato late blight fungus Phytophthora infestans (Mont.) de Bary were isolated from a genomic library in a screen for genes induced in planta. Expression of these genes was studied during pathogenesis on various host tissues and different host plants, some of which show specific resistance against P. infestans infection. During pathogenesis on leaves and tubers of the fully susceptible potato cultivar (cv.) Ajax and on leaves of the fully susceptible tomato cv. Moneymaker, the P. infestans ipiB and ipiO genes show a transient expression pattern with highest mRNA levels in the early stages of infection. During the interaction with leaves of the partially resistant potato cv. Pimpernel, the expression is also transient but accumulation and disappearance of the mRNAs is delayed. Also in P. infestans inoculated onto a race-specific resistant potato cultivar and onto the nonhost Solanum nigrum, ipiB and ipiO mRNA is detectable during the initial stages of infection. Apparently, the expression of the ipiB and the ipiO genes is activated in compatible, incompatible and nonhost interactions. In encysted zoospores, ipiB and ipiO mRNA accumulation was not detectable, but during cyst germination and appressorium formation on an artificial surface the genes are highly expressed. Expression studies in mycelium grown in vitro revealed that during nutrient starvation the expression of the ipiB and ipiO genes is induced. For ipiO gene expression, carbon deprivation appeared to be sufficient. The ipiO gene promoters contain a sequence motif that functions as a glucose repression element in yeast and this motif might be involved in the regulation of ipiO gene expression.

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References

  • Bodenmann J, Heiniger U, Hohl HR (1985) Extracellular enzymes of Phytophthora infestans: endo-cellullase, β-glucosidases, and 1,3-β-glucanases. Can J Microbiol 31:75–82

    Google Scholar 

  • Caten CE, Jinks JL (1968) Spontaneous variability of single isolates of Phytophthora infestans. I. Cultural variation. Can J Bot 46:329–347

    Google Scholar 

  • Choi D, Ward BL, Bostock RM (1992) Differential induction and suppression of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in response to Phytophthora infestans and to its elicitor arachidonic acid. Plant Cell 4:1333–1344

    Google Scholar 

  • Coffey MD, Wilson UE (1983) Histology and cytology of infection and disease caused by Phytophthora. In: Erwin DC, Bartnicki-Garcia S, Tsao PH (eds) Phytophthora: its biology, taxonomy, ecology, and pathology. American Phytopathological Society, St Paul, Minnesota, pp 289–301

    Google Scholar 

  • Cole ALJ (1970) Pectic enzyme activity from Phytophthora infestans. Phytochemistry 9:337–340

    Google Scholar 

  • Colon LT, Eijlander R, Budding DJ, van Ijzendoorn MT, Pieters MJJ, Hoogendoorn J (1993) Resistance to potato late blight (Phytophthora infestans (Mont.) de Bary) in Solanum nigrum, S. villosum and their sexual hybrids with S. tuberosum and S. demissum. Euphytica 66:55–64

    Google Scholar 

  • Feinberg AP, Vogelstein G (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    Google Scholar 

  • Förster H (1988) Pectinesterases from Phytophthora infestans. Methods Enzymol 16:355–361

    Google Scholar 

  • Fritzemeier K-H, Cretin C, Kombrink E, Rohwer F, Taylor J, Scheel D, Hahlbrock K (1987) Transient induction of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase mRNAs in potato leaves infected with virulent or avirulent races of Phytophthora infestans. Plant Physiol 85:34–41

    Google Scholar 

  • Gees R, Hohl HR (1988) Cytological comparison of specific (R3) and general resistance to late blight in potato leaf tissue. Phytopathology 78:350–357

    Google Scholar 

  • Hahlbrock K, Arabatzis N, Becker-André M, Joos H-J, Kombrink E, Schröder M, Strittmatter G, Taylor J (1989) Local and systemic gene activation in fungus-infected potato leaves. In: Lugtenberg BJJ (ed) Signal molecules in plants and plant-microbe interactions. (NATO ASI Series, vol H36) Springer, Berlin, Heidelberg, pp 241–249

    Google Scholar 

  • Henniger H (1959) Versuche zur Kultur verschiedener Rassen von Phytophthora infestans (Mont) de By. auf künstlichen Nährböden. Phytopathol Z 34:285–306

    Google Scholar 

  • Hohl HR (1991) Nutrition. In: Ingram DS, Williams PH (eds) Advances in plant pathology, volume 7; Phytophthora infestans, the cause of late blight of potato. Academic Press, London, San Diego, pp 53–84

    Google Scholar 

  • Jarvis MC, Threlfall DR, Friend J (1981) Potato cell wall polysaccharides: degradation with enzymes from Phytophthora infestans. J Exp Bot 32:1309–1319

    Google Scholar 

  • Judelson HS, Tyler GM, Michelmore RW (1991) Transformation of the oomycete pathogen Phytophthora infestans. Mol Plant-Microbe Interact 4:602–607

    Google Scholar 

  • Judelson HS, Dudler R, Pieterse CMJ, Unkles E, Michelmore RW (1993) Expression and antisense inhibition of transgenes in Phytophthora infestans is modulated by choice of promoter and position effects. Gene 133:63–69

    Google Scholar 

  • Logemann J, Schell J, Willmitzer L (1987) Improved method for the isolation of RNA from plant tissues. Anal Biochem 163:16–20

    CAS  PubMed  Google Scholar 

  • Mahe A, Grisvard J, Dron M (1992) Fungal- and plant-specific gene markers to follow the bean anthracnose infection process and normalize a bean chitinase mRNA induction. Mol PlantMicrobe Interact 5:242–248

    Google Scholar 

  • Martini N, Egen M, Rüntz I, Strittmatter G (1993) Promoter sequences of a potato pathogenesis-related gene mediate transcriptional activity selectively upon fungal infection. Mol Gen Genet 236:179–186

    Google Scholar 

  • Matton DP, Brisson N (1989) Cloning, expression, and sequence conservation of pathogenesis-related gene transcripts of potato. Mol Plant-Microbe Interact 2:325–331

    Google Scholar 

  • Nehlin JO, Ronne H (1990) Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins. EMBO J 9:2891–2898

    Google Scholar 

  • Nehlin JO, Carlberg M, Ronne H (1991) Control of yeast GAL genes by MIG1 repressor: a transcriptional cascade in the glucose response. EMBO J 10:3373–3377

    Google Scholar 

  • Pieterse CMJ, Risseeuw EP, Davidse LC (1991) An in planta induced gene of Phytophthora infestans codes for ubiquitin. Plant Mol Biol 17:799–811

    Google Scholar 

  • Pieterse CMJ, Riach MBR, Bleker T, van den Berg-Velthuis GCM, Govers F (1993a) Isolation of putative pathogenicity genes of the potato late blight fungus Phytophthora infestans by differential screening of a genomic library. Physiol Mol Plant Pathol 43:69–79

    Google Scholar 

  • Pieterse CMJ, Verbakel HM, Hoek Spaans J, Davidse LC, Govers F (1993b) Increased expression of the calmodulin gene of the late blight fungus Phytophthora infestans during pathogenesis on potato. Mol Plant-Microbe Interact 6:164–172

    Google Scholar 

  • Pieterse CMJ, van West P, Verbakel HM, Brassé PWHM, van den Berg-Velthuis GCM, Govers F (1994) Structure and genomic organization of the ipiB and ipi0 gene clusters of Phytophthora infestans. Gene 138:67–77

    Google Scholar 

  • Pristou R, Gallegly ME (1954) Leaf penetration by Phytophthora infestans. Phytopathology 44:81–86

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edition. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Schröder M, Hahlbrock K, Kombrink E (1992) Temporal and spatial patterns of 1,3-β-glucanase and chitinase induction in potato leaves infected by Phytophthora infestans. Plant J 2:161–172

    Google Scholar 

  • Talbot NJ, Ebbole DJ, Hamer JE (1993) Identification and characterization of MPG1 a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. Plant Cell 5:1575–1590

    Google Scholar 

  • Taylor JL, Fritzemeier K-H, Häuser I, Kombrink E, Rohwer F, Schröder M, Strittmatter G, Hahlbrock K (1990) Structural analysis and activation by fungal infection of a gene encoding a pathogenesis-related protein in potato. Mol Plant-Microbe Interact 3:72–77

    Google Scholar 

  • Tomiyama K (1963) Physiology and biochemistry of disease resistance of plants. Annu Rev Phytopathol 1:295–324

    Google Scholar 

  • Umaerus V (1970) Studies on field resistance to Phytophthora infestans. 5. Mechanisms of resistance and application to potato breeding. Z Pflanzenziichtung 63:1–23

    Google Scholar 

  • Unkles SE, Moon RP, Hawkins AR, Duncan JM, Kinghorn JR (1991) Actin in the oomycetous fungus Phytophthora infestans is the product of several genes. Gene 100:105–112

    Google Scholar 

  • van den Ackerveken GFJM, Dunn RM, Cozijnsen TJ, Vossen IPMJ, van den Broek HWJ, de Wit PJGM (1994) Nitrogen limitation induces expression of the avirulence gene avr9 in the fungal tomato pathogen Cladosporium fulvum. Mol Gen Genet 243:277–285

    Google Scholar 

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Author notes

  1. Corné M. J. Pieterse

    Present address: Department of Plant Ecology and Evolutionary Biology, University of Utrecht, P.O. Box 800.84, 3508 TB, Utrecht, The Netherlands

Authors and Affiliations

  1. Department of Phytopathology, Wageningen Agricultural University, Binnenhaven 9, P.O. Box 8025, 6700 EE, Wageningen, The Netherlands

    Corné M. J. Pieterse, Anne-Marie C. E. Derksen, Jindra Folders & Francine Govers

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  1. Corné M. J. Pieterse
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  2. Anne-Marie C. E. Derksen
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  3. Jindra Folders
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  4. Francine Govers
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Communicated by C. van den Hondel

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Pieterse, C.M.J., Derksen, AM.C.E., Folders, J. et al. Expression of the Phytophthora infestans ipiB and ipi0 genes in planta and in vitro. Molec. Gen. Genet. 244, 269–277 (1994). https://doi.org/10.1007/BF00285454

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  • DOI: https://doi.org/10.1007/BF00285454

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