Summary
The tandem repeat of a 28-base-pair (bp) sequence downstream of the human c-Ha-ras-1 oncogene was studied as a probe for DNA fingerprinting. Multiple hypervariable patterns were observed by Southern hybridization at low stringency. The patterns were specific to individuals, indicating the availability of the 28-bp repeat as a probe for DNA fingerprinting. Moreover, we cloned the tandem repeat of a 33-bp sequence, which cross-hybridized with the 28-bp repeat. This 33-bp repeat detected another set of hypervariable restriction fragments by Southern hybridization at the same stringency. These results suggests that ‘probe walking’ can be employed to develop novel probes that provide different DNA fingerprints.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Burke T, Bruford MW (1987) DNA fingerprinting in birds. Nature 327:149–152
Buroker N, Bestwick R, Haight G, Magenis RE, Litt M (1987) A hypervariable repeated sequence on human chromosome 1p36. Hum Genet 77:175–181
Capon DJ, Chen EY, Levinson AD, Seeburg PH, Goeddel DV (1983) Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue. Nature 302:33–37
Colb M, Yang-Feng T, Francke U, Mermer B, Parkinson DR, Krontiris TG (1986) A variable tandem repeat locus mapped to chromosome band 10q26 is amplified and rearranged in leukocyte DNAs of two cancer patients. Nucleic Acids Res 14:7929–7937
Donis-Keller H, Green P, Helms C, Cartinhour S, Weiffenbach B, Stephens K, Keith TP, Bowden DW, Smith DR, Lander ES, Botstein D, Akots G, Rediker KS, Gravius T, Brown VA, Rising MB, Parker C, Powers JA, Watt DE, Kauffman ER, Bricker A, Phipps P, Muller-Kahle H, Fulton TR, Ng S, Schumm JW, Braman JC, Knowlton RG, Barker DF, Crooks SM, Lincoln SE, Daly MJ, Abrahamson J (1987) A genetic linkage map of the human genome. Cell 51:319–337
Fowler SJ, Gill P, Werrett DJ, Higgs DR (1988) Individual specific DNA fingerprints from a hypervariable region probe: alpha-globin 3′ HVR. Hum Genet 79:142–146
Georges M, Cochaux P, Lequarre AS, Young MW, Vassart G (1987) DNA fingerprinting in man using a mouse probe related to part of the Drosophila ‘Per’ gene. Nucleic Acids Res 15:7193
Gill P, Jeffreys AJ, Werrett DJ (1985) Forensic application of DNA ‘fingerprints’. Nature 318:577–579
Hill AVS, Jeffreys AJ (1985) Use of minisatellite DNA probes for determination of twin zygosity at birth. Lancet I:1394–1395
Hoelzel AR, Amos W (1988) DNA fingerprinting and ‘scientific’ whaling. Nature 333:305
Honjo T, Obata M, Yamawaki-Kataoka Y, Kataoka T, Kawakami T, Takahashi N, Mano Y (1979) Cloning and complete nucleotide sequence of mouse immunoglobulin γ 1 chain gene. Cell 18:559–568
Jeffreys AJ, Morton DB (1987) DNA fingerprints of dogs and cats. Anim Genet 18:1–15
Jeffreys AJ, Wilson V, Thein SL (1985a) Hypervariable ‘minisatellite’ regions in human DNA. Nature 314:67–73
Jeffreys AJ, Wilson V, Thein SL (1985b) Individual-specific ‘fingerprints’ of human DNA. Nature 316:76–79
Jeffreys AJ, Brookfield JFY, Semeonoff R (1985c) Positive identification of an immigration test-case using human DNA fingerprints. Nature 317:818–819
Jeffreys AJ, Wilson V, Thein SL, Weatherall DJ, Ponder BAJ (1986) DNA “fingerprints” and segregation analysis of multiple markers in human pedigrees. Am J Hum Genet 39:11–24
Krontiris TG, DiMartino NA, Colb M, Parkinson DR (1985) Unique allelic restriction fragments of the human Ha-ras locus in leukocyte and tumour DNAs of cancer patients. Nature 313:369–374
Nakamura Y, Leppert M, O'Connell P, Wolff R, Holm T, Culver M, Martin C, Fujimoto E, Hoff M, Kumlin E, White R (1987) Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235:1616–1622
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Shih C, Weinberg RA (1982) Isolation of a transforming sequence from a human bladder carcinoma cell line. Cell 29:161–169
Simmler MC, Johnsson C, Petit C, Rouyer F, Vergnaud G, Weissenbach J (1987) Two highly polymorphic minisatellites from the pseudoautosomal region of the human sex chromosomes. EMBO J 6:963–969
Thein SL, Jeffreys AJ, Blacklock HA (1986) Identification of posttransplant cell population by DNA fingerprint analysis. Lancet II:37
Thein SL, Jeffreys AJ, Gooi HC, Cotter F, Flint J, O'Connor NTJ, Wainscoat JS (1987) Detection of somatic changes in human cancer DNA by DNA fingerprint analysis. Br J Cancer 55:353–356
Vassart G, Georges M, Monsieur R, Brocas H, Lequarre AS, Christophe D (1987) A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA. Science 235:683–684
Vieira J, Messing J (1987) Production of single-stranded plasmid DNA. Methods Enzymol 153:3–11
Washio K, Misawa S, Ueda S (1989) Individual identification of nonhuman primates using DNA fingerprinting. Primates 30:217–221
Wetton JH, Carter RE, Parkin DT, Walters D (1987) Demographic study of a wild house sparrow population by DNA fingerprinting. Nature 327:147–149
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Washio, K., Misawa, S. & Ueda, S. Probe walking: development of novel probes for DNA fingerprinting. Hum Genet 83, 223–226 (1989). https://doi.org/10.1007/BF00285160
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00285160