Summary
The gliadin proteins of the hexaploid bread wheat cultivar ‘Cheyenne’ (Triticum aestivum L. var. aestivum), which has good mixing and baking characteristics, were analyzed by 2-dimensional (2-pH) polyacrylamide gel electrophoresis (pH 3.2 in the 1st dimension, pH 9.2 in the 2nd). Genes for most of the 35 resolved components were assigned to the chromosomes of homoeologous groups 1 and 6 through the use of various aneuploids and substitution lines. A similar analysis was carried out for the cultivar ‘Chinese Spring’, which does not have good quality, and which had been analyzed by a different 2-dimensional procedure by Wrigley and Shepherd (Ann NY Acad Sci 209:154, 1973). A coordinate system was devised for description of the components in the 2-dimensional patterns.
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Abbreviations
- 1-dimensional:
-
one-dimensional
- 2-dimensional:
-
two-dimensional
- 2-pH:
-
two-pH
- 1st:
-
first
- 2nd:
-
second
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Communicated by J. MacKey
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Lafiandra, D., Kasarda, D.D. & Morris, R. Chromosomal assignment of genes coding for the wheat gliadin protein components of the cultivars ‘Cheyenne’ and ‘Chinese Spring’ by two-dimensional (two-pH) electrophoresis. Theoret. Appl. Genetics 68, 531–539 (1984). https://doi.org/10.1007/BF00285007
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DOI: https://doi.org/10.1007/BF00285007