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Developmental studies in Drosophila

III. The puffing patterns of the salivary gland chromosomes of D. pseudoobscura

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Abstract

A study of the puffing patterns of the salivary gland chromosomes of D. pseudoobscura was carried out through several larval, prepupal, and pupal stages of development. A total of 176 puffs were found, 111 of which changed during the stages studied. As described in previous investigations with other Drosophila species there are two major peaks of puffing activity. These two peaks occur during puparium formation and pupation. Additionally, a minor activity-peak occurs during mid-prepupal life. Attempts have been made to establish correlations between the puffing data and those obtained from electrophoretic and ultrastructural studies.

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References

  • Ashburner, M.: Patterns of puffing activity in the salivary gland chromosomes of Drosophila. I. Autosomal puffing patterns in a laboratory stock of D. melanogaster. Chromosoma (Berl.) 21, 398–428 (1967).

    Google Scholar 

  • Ashburner, M.: Function and structure of polytene chromosomes during insect development. Advanc. Ins. Physiol. 7, 1–95 (1970).

    Google Scholar 

  • Barritt, L. C., Birt, L. M.: Prothoracic gland hormone in the sheep blowfly, Lucilia cuprina. J. Insect. Physiol. 16, 671–677 (1970).

    Google Scholar 

  • Baudisch, W., Panitz, R.: Kontrolle eines biochemischen Merkmals in den Speicheldrüsen von Acricotopus lucidus durch einen Balbiani ring. Exp. Cell Res. 49, 470–476 (1968).

    Google Scholar 

  • Becker, H.-J.: Die Puffs der Speicheldrüsenchromosomen von Drosophila melanogaster. I. Beobachtungen zum Verhalten des Puffmusters im Normalstamm und bei zwei Mutanten, giant und lethal-giant-larvae. Chromosoma (Berl.) 10, 654–678 (1959).

    Google Scholar 

  • Becker, H.-J.: Die Puffs der Speicheldrüsenchromosomen von Drosophila melanogaster. II. Die Auslösung der Puffbildung, ihre Specificität und ihre Beziehung zur Funktion der Ringdrüse. Chromosoma (Berl.). 13, 341–384 (1962).

    Google Scholar 

  • Beermann, W.: Chromomerenkonstanz und specifische Modifikationen der Chromosomenstruktur in der Entwicklung und Organdifferenzierung von Chironomus tentans. Chromosoma (Berl.) 5, 139–198 (1952).

    Google Scholar 

  • Beermann, W.: Ein Balbiani-Ring als Locus einer Speicheldrüsenmutation. Chromosoma (Berl.) 12, 1–25 (1961).

    Google Scholar 

  • Berendes, H. D.: Salivary gland function and chromosomal puffing patterns in Drosophila hydei. Chromosoma (Berl.) 17, 35–77 (1965a).

    Google Scholar 

  • Berendes, H. D.: The induction of changes in chromosomal activity in different polytene types of cell in Drosophila hydei. Develop. Biol. 11, 371–384 (1965b).

    Google Scholar 

  • Berendes, H. D.: Gene activities in the Malphigian tubules of Drosophila hydei at different developmental stages. J. exp. Zool. 162, 209–218 (1966).

    Google Scholar 

  • Berendes, H. D.: The hormone ecdysone as effector of specific changes in the pattern of gene activities of Drosophila hydei. Chromosoma (Berl.) 22, 274–293 (1967).

    Google Scholar 

  • Breuer, M. E., Pavan, C.: Gens na differenciação. Ciên. Cult. (São Paulo) 4, 141 (1952).

    Google Scholar 

  • Burnet, B., Hartmann-Goldstein, I.: Environmental influences on puffing in the salivary gland chromosomes of Drosophila melanogaster. Genetics Res. (Camb.) 7, 113–124 (1971).

    Google Scholar 

  • Butenandt, A., Karlson, P.: Über die Isolierung eines Metamorphose Hormones der Insekten in Kristallisierter Form. Z. Naturforsch. 9b, 389–391 (1954).

    Google Scholar 

  • Clever, U.: Genaktivitäten in den Riesenchromosomen von Chironomus tentans und ihre Beziehungen zur Entwicklung. II. Das Verhalten der Puffs während des letzen Larvenstadiums und der Puppenhautung. Chromosoma (Berl.) 13, 385–436 (1962a).

    Google Scholar 

  • Clever, U.: Genaktivitäten in den Riesenchromosomen von Chironomus tentans und ihre Beziehungen zur Entwicklung. III. Das Aktivitätsmuster in Phasen der Entwicklungsruhe. J. Insect Physiol. 8, 357–376 (1962b).

    Google Scholar 

  • Clever, U.: Genaktivitäten in den Riesenchromosomen von Chironomus tentans und ihre Beziehungen zur Entwicklung. IV. Das Verhalten der Puffs in der Larvenhautung. Chromosoma (Berl.) 14, 651–675 (1963).

    Google Scholar 

  • Clever, U.: Puffing in giant chromosomes of Diptera and the mechanism of its control. In: The nucleohistones (J. Bonner and P. Ts'o, eds.), p. 317–334. San Francisco: Holden Day Inc. 1964.

    Google Scholar 

  • Clever, U., Karlson, P.: Induktion von Puff-Veränderungen in den Speicheldrüsenchromosomen von Chironomus tentans durch Ecdyson. Exp. Cell Bes. 20, 632–626 (1960).

    Google Scholar 

  • Conn, H. J., Darrow, M. A., Emmel, V. M.: Staining procedures used by the Biological Stain Commission. Baltimore: Williams and Wilkins Co. 1965.

    Google Scholar 

  • Doane, W.: Isoamylases in Drosophila hydei: a system for the analysis of genespecific puffing activity. Dros. Inf. Serv. 47, 100 (1971).

    Google Scholar 

  • Dobzhansky, Th.: Genetics of the evolutionary process. New York: Columbia University Press 1970.

    Google Scholar 

  • Doyle, D., Laufer, H.: Analysis of secretory processes in Dipteran salivary glands. In: In vitro. A symposium of the Tissue Culture Association, vol. 3, Differentiation and defense mechanisms in lower organisms (M. M. Sigel, ed.), p. 93–103. Baltimore: Waverly Press 1968.

    Google Scholar 

  • Geiger, H. R., Mitchell, H. K.: Salivary gland function in phenoloxidase production in Drosophila melanogaster. J. Insect Physiol. 12, 747–754 (1966).

    Google Scholar 

  • Harrod, M. J. E.: An ultrastructural study of some stages of salivary gland development in Drosophila pseudoobscura. Ph. D. Dissertation, The University of Texas Southwestern Medical School at Dallas 1971.

    Google Scholar 

  • Harrod, M. J. E., Kastritsis, C. D.: Developmental studies in Drosophila II. Ultrastructural analysis of the salivary glands of Drosophila pseudoobscura during some stages of development. J. Ultrastruct. Res. (in press 1971).

  • Kastritsis, C. D.: A comparative chromosome study in the incipient species of the Drosophila paulistorum complex. Chromosoma (Berl.) 19, 208–222 (1966).

    Google Scholar 

  • Kastritsis, C. D., Crumpacker, D. W.: Gene arrangements in the third chromosome of Drosophila pseudoobscura. I. Configuration with tester chromosomes. J. Hered. 57, 150–158 (1966).

    Google Scholar 

  • Laufer, H., Holt, T. K. H.: Juvenile hormone effects on chromosomal puffing and development in Chironomus thummi. J. exp. Zool. 173, 341–352 (1970).

    Google Scholar 

  • Laufer, H., Nakase, Y.: Salivary gland secretion and its relation to chromosomal puffing in the Dipteran, Chironomus thummi. Proc. nat. Acad. Sci. (Wash.) 53, 511–516 (1965).

    Google Scholar 

  • Lesher, S.: Studies on the larval salivary glands of Drosophila. III. The histochemical localization and possible significance or ribonucleic acid, alkaline phosphatase, and polysaccharide. Anat. Rec. 114, 633–665 (1952).

    Google Scholar 

  • Leuchtenberger, C.: Quantitative determination of DNA in cells by Feulgen microspectrophotometry. In: General cytochemical methods (J. F. Danielli, ed.), vol. 1, p. 219–278. New York: Academic Press 1963.

    Google Scholar 

  • Pasteur, N.: Microelectrophoretic analysis of enzymes and other proteins during development of Drosophila pseudoobscura. Ph. D. Dissertation, The University of Texas Southwestern Medical School at Dallas, 1971.

    Google Scholar 

  • Pasteur, N., Kastritsis, C. D.: Developmental studies in Drosophila. I. Acid phosphatases, esterases, and other proteins in organs and whole-fly homogenates during development of D. pseudoobscura. Develop. Biol. (in press, 1971a).

  • Pasteur, N., Kastritsis, C. D.: Developmental studies in Drosophila. IV. Quantitative protein changes in organs and whole-fly homogenates during development of D. pseudoobscura. Experientia (Basel) (in press, 1971b).

  • Pavan, C., Breuer, M. E.: Differences in nucleic acid content of the loci in polytene chromosomes of Rhychosciara angelae according to tissue and larval stages. In: Symposium on Cell Secretion, (G. Schreiber, ed.), p. 90. Belo Horizonte: University Minas Gerais Press 1955.

    Google Scholar 

  • Perkowska, E.: Some characteristics of the salivary gland secretion of Drosophila virilis. Exp. Cell Res. 32, 259–271 (1963).

    Google Scholar 

  • Poels, C. L. M.: Time sequence in the expression of various developmental characters induced by ecdysterone in Drosophila hydei. Develop. Biol. 23, 210–225 (1970).

    Google Scholar 

  • Poels, C. L. M., Loof, A. de, Berendes, H. D.: Functional and structural changes in Drosophila salivary gland cells triggered by ecdysterone. J. Insect Physiol. 17, 1717–1729 (1971).

    Google Scholar 

  • Shaaya, E., Karlson, P.: Der Ecdysontiter während der Insektentwicklung. II. Die Postembryonale Entwicklung der Schmeissfliege Calliphora erythrocephala. Meig. J. Insect Physiol. 11, 65–69 (1965).

    Google Scholar 

  • Slizynski, B. M.: Functional changes in polytene chromosomes of Drosophila melanogaster. Cytologia (Tokyo) 29, 330–336 (1964).

    Google Scholar 

  • Stocker, A. J., Jackson, J.: A technique for the synchronization of Drosophila for developmental studies. Dros. Inf. Serv. 46, 157 (1971).

    Google Scholar 

  • Tan, C. C.: The cytological maps of the autosomes in Drosophila pseudoobscura. Z. Zellforsch. 26, 439–461 (1937).

    Google Scholar 

  • Whitten, J. M.: Haemocytes and the metamorphosing tissues in Sarcophaga bullata, Drosophila melanogaster, and other cyclorrhaphous Diptera. J. Insect Physiol. 10, 447–469 (1964).

    Google Scholar 

  • Whitten, J. M.: Coordinated development in the foot pad of the fly Sarcophaga bullata during metamorphosis: changing puffing patterns of the giant cell chromosomes. Chromosoma (Berl.) 26, 215–244 (1969a).

    Google Scholar 

  • Whitten, J. M.: Haemocyte activity in relation to epidermal cell growth, cuticule secretion, and cell death in a metamorphosing cyclorraphous pupa. J. Insect Physiol. 15, 763–778 (1969b).

    Google Scholar 

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Author notes

  1. Ann Jacob Stocker

    Present address: Department of Zoology, The University of Texas at Austin, 78712, Austin, Texas, USA

Authors and Affiliations

  1. Department of Cell Biology, The University of Texas Southwestern Medical School, Dallas

    Ann Jacob Stocker & Costas D. Kastritsis

Authors
  1. Ann Jacob Stocker
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  2. Costas D. Kastritsis
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Additional information

Supported in part by grants GM-16736-03 and FR-05426-09 from the U.S. Department of Health, Education, and Welfare. Ann Jacob Stocker was a holder of a University of Texas predoctoral fellowship.

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Stocker, A.J., Kastritsis, C.D. Developmental studies in Drosophila . Chromosoma 37, 139–176 (1972). https://doi.org/10.1007/BF00284936

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  • DOI: https://doi.org/10.1007/BF00284936

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