Abstract
The growth rates of two diatoms, acidophilic Asterionella ralfsii and circumneutral A. formosa, were differentially affected by varying pH, Al, and EDTA in chemically defined media. Free Al ion concentration increased as pH and EDTA concentration decreased. Free trace metal ion concentration decreased as EDTA levels increased but increased by orders of magnitude upon addition of Al. pH had an overriding species specific effect on growth rate; at low pH A. ralfsii had higher growth rates than A. formosa and vice versa at high pH. For both species higher EDTA levels depressed growth rates. Moderate additions of Al generally resulted in growth stimulation. The growth rate stimulations, especially at 200 and 400 μg L−1 Al additions, correlate to increases in free trace metal ion concentrations. The EDTA-AI interaction effects on growth rate were both pH and concentration dependent: at pH 7 both species were stimulated by addition of Al at all EDTA levels (except A. ralfsii at 5.0 mM EDTA and A. formosa at 0.5 mNM EDTA); at pH 6 Al addition either stimulated or had no effect on the growth rates of both species (except at low EDTA and high Al levels); at pH 5 A. formosa did not grow and additions of 200 μg L−1 Al stimulated growth of A. ralfsii. It is likely that the effect of pH, Al, and EDTA on speciation of essential or toxic trace metals affects growth rates of these diatoms in a species specific manner.
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References
Baccini, P.: 1984, ‘Regulation of Trace Metal Concentrations in Fresh Water Systems’, in M. Dekker (ed.), Metal Ions in Biological Systems, H. Sigel Pub., N.Y., 18, pp. 268–283.
Brand, L. E., Guillard, R. R. L., and Murphy, L. S.: 1981, J. Plank. Res. 3, 193.
Campbell, P. G. and Stokes, P. M.: 1985, Can. J. Fish. Aquat. Sci. 43, 2034.
Charles, D. F.: 1985, Ecology 66, 994.
Dougan, W. K. and Wilson, A. L.: 197, Analyst 99, 413.
Driscoll, C. T., Baker, J. P., Bisgoni, J. J., and Schofield, C. C.: 1980, Nature 284, 161.
Driscoll, C. T., Baker, J. P., Biwgoni, J. J., and Schofield, C. C.: 1984, ‘Aluminum Speciation and Equilibria in Dilute Acidified Waters of the Adirondack Region of New York State’, in O. Bricker (ed.), Acid Precipitation: Geological Aspects, Ann Arbor Sci. Pub., Ann Arbor, MI, pp. 55–75.
Driscoll, C. T. and Newton, R. M.: 1985, Environ. Sci. Technol. 19, 1018.
Eichenberger, E.: 1985, ‘The Interrelationships Between Essentiality and Toxicity of Metals in the Aquatic Ecosystem’, in H. Sigel (ed.), Metal Ions in Biological Systems, M. Dekker Pub., N.Y., 20, pp. 67–96.
Folsom, B. R., Popescue, N. A., and Wood, J. M.: 1986, Environ. Sci. Technol. 20, 616.
Gamble, D. S. and Schnitzer, M.: 1973, ‘The Chemistry of Fulvic Acid and its Reaction with Metal Ions’, in P. Singer (ed.), Trace Metal and Metal Organic Interactions in Natural Water, Ann Arbor Sci. Pub., Ann Arbor, MI, pp. 265–302.
Gensemer, R. W.: 1989, Ph.D. Dissertation, University of Michigan, 159 p.
Gensemer, R. W.: 1990, J. Phycol. 26, 250.
Gensemer, R. W. and Kilham, S. S.: 1984, Can. J. Fish. Aquat. Sci. 41, 1240.
Hamilton, R. D.: 1972, ‘The Environmental Acceptability of NTA: Current Research and Areas of Concern’, in G. E. Likens (ed.), Nutrients and Eutrophication Special Symposium, Allen Press, Lawrence, KS, 1, p. 217–221.
Helliwell, S., Batley, G. E., Florence, T. M., and Lumsden, B. G.: 1983, Environ. Technol. Lett. 4, 141.
Hongve, D., Skogheim, O. K., Hindar, A., and Abrahamsen, H.: 1980, Bull. Environ. Contam. Toxicol. 25, 594.
Huljev, D. and Strohal, P.: 1983, Mar. Biol. 73, 243.
Jackson, T. A. and Hecky, R. E.: 1980, Can. J. Fish. Aquat. Sci. 37, 2300.
Jorgensen, S. E. and Jensen, A.: 1984, ‘Processes of Metal Ions in the Environment’, in H. Sigel (ed.), Metal Ions in Biological Systems, M. Dekker Pub., N.Y., 18, p. 69–85.
LaZerte, B. D.: 1984, Can. J. Fish. Aquat. Sci. 41(5), 766.
LaZerte, B. D., Chun, C, and Evans, D.: 1988, Environ. Sci. Technol. 22, 1106.
Mantoura, R. F. C., Dickson, A., and Riley, J. P.: 1977, Estuar. Coast. Mar. Sci. 6, 387.
Morel, F. M. M., Westall, J. C., Rueter, J. G., and Chaplick, J. P.: 1975, ‘Description of the Algal Growth Media “AQUIL” and “FRAQUIL”, Technical Note No. 16, Water Quality Laboratory, R. M. Parsons Laboratory, Massachusetts Institute of Technology, Cambridge, MA.
Nalewajko, C. and Paul, B.: 1985, Can. J. Fish. Aquat. Sci. 42, 1946.
Peterson, H. G. and Healey, F. P.: 1985, J. Phycol. 21, 217–222.
Peterson, H. G., Healey, F. P., and Wageman, R.: 1984, Can. J. Fish. Aquat. Sci. 41, 974.
Rueter, J. G., O'Reilly, K. T., and Petersen, R. R. 1987, Environ. Sci. Technol. 2, 435. Schindler, D. W.: 1988, Science (Wash. D.C.) 239, 149.
Steinberg, C. and Meunster, U.: 1985, ‘Geochemistry of Humic Substances in Lake Water’, in G. R. Aiken (ed.), Soil, Sediments, and Water, J. Wiley and Sons Pub., N.Y., pp. 105–147.
Sullivan, T. J. and Seip, H. M.: 1986, Int. J. Environ. Anal. Chem. 26, 61.
Sunda, W. G. and Guillard, R. R. L.: 1976, J. Mar. Res. 34, 511.
Sunda, W. G. and Lewis, J. A. M. 1978, Limnol Oceanog. 23, 870.
Thurman, E. M,: 1985, Organic Geochemistry of Natural Waters, Martinus Nijhoff/Dr. W. Junk Pub., Dordrecht, The Netherlands, pp. 15–25, 51–58, 410–420.
Westall, J., Zachary, J., and Morel, F. M. M.: 1976, Technical Note No. 18, R. M. Parsons Laboratory, Massachusetts Institute of Technology, Cambridge, MA.
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Riseng, C.M., Gensemer, R.W. & Kilham, S.S. The effect of pH, aluminum, and chelator manipulations on the growth of acidic and circumneutral species of Asterionella . Water Air Soil Pollut 60, 249–261 (1991). https://doi.org/10.1007/BF00282626
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DOI: https://doi.org/10.1007/BF00282626