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Assignment of the gene coding for human β-glucocerebrosidase to the region q21-q31 of chromosome 1 using monoclonal antibodies

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Summary

A series of man-Chinese hamster somatic cell hybrids with a variable content of human chromosomes was used to study the localization of the human gene coding for the lysosomal enzyme β-glucocerebrosidase (EC 3.2.1.45). In lysates made from hybrid cells, the human enzyme was specifically recognized by a mouse monoclonal antibody raised against human placental β-glucocerebrosidase. This monoclonal antibody did not cross-react with Chinese hamster β-glucocerebrosidase. After reaction of the antibody with the enzyme, β-glucocerebrosidase was precipitated by addition of Protein A-Sepharose beads, and was detected on the beads by its enzymatic activity. From the analysis of a series of man-Chinese hamster hybrids, among which were hybrids with specific segments of chromosome 1, we conclude that the gene coding for human β-glucocerebrosidase is localized in the region q21-q31 of chromosome 1.

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Authors and Affiliations

  1. Department of Cell Biology and Genetics, Erasmus University Rotterdam, P. O. Box 1738, NL-3000 DR, Rotterdam, The Netherlands

    R. A. Barneveld, W. Keiizer, A. Geurts van Kessel, H. Galjaard, A. Westerveld & A. J. J. Reuser

  2. Laboratory of Biochemistry, B.C.P. Jansen Institute, University of Amsterdam, Plantage Muidergracht 12, NL-1018 TV, Amsterdam, The Netherlands

    F. P. W. Tegelaers & J. M. Tager

  3. Medical and Molecular Genetics Section, Developmental and Metabolic Neurology Branch, National Institutes of Health, 20205, Bethesda, MD, USA

    E. I. Ginns, R. O. Brady & J. A. Barranger

Authors
  1. R. A. Barneveld
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  2. W. Keiizer
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  3. F. P. W. Tegelaers
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  4. E. I. Ginns
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  5. A. Geurts van Kessel
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  6. R. O. Brady
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  7. J. A. Barranger
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  8. J. M. Tager
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  9. H. Galjaard
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  10. A. Westerveld
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  11. A. J. J. Reuser
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Barneveld, R.A., Keiizer, W., Tegelaers, F.P.W. et al. Assignment of the gene coding for human β-glucocerebrosidase to the region q21-q31 of chromosome 1 using monoclonal antibodies. Hum Genet 64, 227–231 (1983). https://doi.org/10.1007/BF00279398

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  • DOI: https://doi.org/10.1007/BF00279398

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