Summary
Results are reported on the production of banding patterns stained with Giemasa. They are obtained from the application of different dyes and dye mixtures to metaphase chromosomes which were pretreated in various ways. The discussion of our findings in addition to those of other authors allows the following conclusions:
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1.
Alterations of the DNA-protein complex determine the production of all banding patterns stained with Giemsa.
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2.
In addition to the accordance between base ratio of the DNA and banding pattern, there exists a base specific attachement of qualitative different non-histone proteins to DNA.
Zusammenfassung
Es wird über Befunde zur Entstehung von Bandenmustern berichtet, die durch verschiedene Färbungen unterschiedlich vorbehandelter Metaphasechromosomen gewonnen wurden. Aus der Diskussion dieser Befunde im Zusammenhang mit den Befunden anderer Autoren ergibt sich:
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1.
Eine Alteration des DNA-Protein-Komplexes ist bestimmend für alle Banden, die sich mit Giemsa anfärben lassen.
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2.
Über eine Entsprechung von Basenverhältnis der DNA und Bandenmustern hinaus existiert eine dem Basenverhältnis spezifisch zugeordnete Besetzung der DNA mit sauren Kernproteinen.
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Vogel, W., Faust, J., Schmid, M. et al. On the relevance of non-histone proteins to the production of Giemsa banding patterns on chromosomes. Hum Genet 21, 227–236 (1974). https://doi.org/10.1007/BF00279017
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DOI: https://doi.org/10.1007/BF00279017