Summary
Cell membranes of vascular smooth muscles of the circle of Willis were studied in thin sections and freeze-replicas. The cell membranes were differentiated into a caveolae intracellulares zone and caveolae-free zone, both of which were generally arranged in an alternate manner and parallel to the major axis of the smooth muscle cell. In the former zone, the caveolae intracellulares, about 600 Å in diameter, were neatly oriented in one to several rows running parallel to the longitudinal axis of the muscle cell with a centre-to-centre distance of about 800 Å. The latter zone was of variable width and smooth, apart from membrane particles or scattered caveolae, and corresponded mainly to the dense area and partially to the myofibril area beneath the cell membrane. Membrane particles were generally more numerous on face A than on face B, and their average number per μm2 was about twice as many inside the rows of the caveolae as outside. Rosette formations of membrane particles were often evident at the stomal rims of the caveolae. Adherentes and gap junctions were occasionally found on the caveolae-free areas which often protruded externally. Tight junctions appeared as a collection of scattered strands, which frequently showed free ends and were parallel to each other and also to the major axis of the smooth muscle cell.
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References
Abe, Y., Tomita, T.: Cable properties of smooth muscle. J. Physiol. (Lond.) 196, 87–100 (1968)
Barr, L., Berger, W., Dewey, M.M.: Electrical transmission at the nexus between smooth muscle cells. J. gen. Physiol. 51, 347–368 (1968)
Bennett, M.R., Rogers, D.G.: A study of the innervation of the taenia coli. J. Cell Biol. 33, 573–596 (1967)
Bozler, E.: Electric stimulation and conduction of excitation in smooth muscle. Amer. J. Physiol. 122, 614–623 (1938)
Büssow, H., Wulfhekel, U.: Die Feinstruktur der glatten Muskelzellen in den großen muskulären Arterien der Vögel. Z. Zellforsch. 125, 339–352 (1972)
Burnstock, G.: Structure of smooth muscle and its innervation. In: Smooth muscle, ed. by Bülbring, E., Brading, A.F., Johnes, A.W., Tomita, T., pp. 2–69, 1. ed. London: Edward Arnold Ltd. 1970
Caesar, R., Edwards, G.A., Ruska, H.: Architecture and nerve supply of mammalian smooth muscle tissue. J. biophys. biochem. Cytol. 3, 867–878 (1957)
Chalcroft, J.P., Bullivant, S.: An interpretation of liver cell membrane and junction structure based on observation of freeze-fracture replicas of both sides of the fracture. J. Cell Biol. 47, 49–60 (1970)
Claude, P., Goodenough, D.A.: Fracture faces of zonulae occludentes from “tight” and “leaky” epithelia. J. Cell Biol. 58, 390–400 (1973)
Cliff, W.J.: The aortic tunica media in growing rats studied with the electron microscope. Lab. Invest. 17, 599–615 (1967)
Cobb, J.L.S., Bennett, T.: A study of nexuses in visceral smooth muscle. J. Cell Biol. 41, 287–297 (1969)
Devine, C.E., Simpson, F.O.: The morphological basis for the sympathetic control of blood vessels. N.Z. med. J. 67, 326–334 (1968)
Devine, C.E., Simpson, F.O., Bertaud, W.S.: Surface features of smooth muscle cells from the mesenteric artery and vas deferens. J. Cell Sci. 8, 421–443 (1971)
Dewey, M.M., Barr, L.: Intercellular connection between smooth muscle cells: the nexus. Science (Wash.) 237, 670–672 (1962)
Dewey, M.M., Barr, L.: A study of the structure and distribution of the nexus. J. Cell Biol. 23, 553–585 (1964)
Friend, D.S., Gilula, N.B.: Variations in tight and gap junctions in mammalian tissues. J. Cell Biol. 53, 758–776 (1972)
Gabella, G.: Caveolae intracellulares and sarcoplasmic reticulum in smooth muscle. J. Cell Sci. 8, 601–609 (1971)
Goodenough, D.A., Revel, J.-P.: A fine structural analysis of intercellular junctions in the mouse liver. J. Cell Biol. 45, 272–290 (1970)
Haudenschild, C., Baumgartner, H.R., Studer, A.: Significance of fixation procedure for preservation of arteries. Experientia (Basel) 28, 828–831 (1972)
Iwayama, T.: Nexuses between areas of the surface membrane of the same arterial smooth muscle cell. J. Cell Biol. 49, 521–525 (1971)
McNutt, N.S., Weinstein, R.S.: Membrane ultrastructure at mammalian intercellular junctions. Progr. Biophys. molec. Biol. 26, 47–101 (1973)
Muggli, R., Baumgartner, H.R.: Pattern of membrane invaginations at the surface of smooth muscle cells of rabbit arteries. Experientia (Basel) 28, 1212–1214 (1972)
Oosaki, T., Ishii, S.: Junctional structure of smooth muscle cells. J. Ultrastruct. Res. 10, 567–577 (1964)
Orci, L., Perrelet, A.: Membrane-associated particles: Increase at sites of pinocytosis demonstrated by freeze-etching. Science (Wash.) 181, 868–869 (1973)
Pease, D.C., Molinari, S.: Electron microscopy of muscular arteries; pial vessels of the cat and monkey. J. Ultrastruct. Res. 3, 447–468 (1960)
Prosser, C.L., Burnstock, G., Kahn, J.: Conduction in smooth muscle: Comparative structural properties. Amer. J. Physiol. 199, 545–552 (1960)
Rhodin, J.A.G.: Fine structure of vascular walls in mammals: With special reference to smooth muscle component. Physiol. Rev. 42, Suppl. 5, 48–81 (1962)
Rhodin, J.A.G.: The ultrastructure of mammalian arterioles and precapillary sphincters. J. Ultrastruct. Res. 18, 181–223 (1967)
Satir, B., Schooley, C., Satir, P.: Membrane reorganization during secretion in Tetrahymena. Nature (Lond.) 235, 53–54 (1972)
Satir, B., Schooley, C., Satir, P.: Membrane fusion in a model system. Mucocyst secretion in Tetrahymena. J. Cell Biol. 56, 153–176 (1973)
Simionescu, M., Simionescu, N., Palade, G.E.: Morphometric data on the endothelium of blood capillaries. J. Cell Biol. 60, 128–152 (1974)
Simionescu, M., Simionescu, N., Palade, G.E.: Segmental differentiations of cell junctions in the vascular endothelium. The microvasculature. J. Cell Biol. 67, 863–885 (1975)
Simionescu, M., Simionescu, N., Palade, G.E.: Segmental differentiations of cell junctions in the vascular endothelium. Arteries and veins. J. Cell Biol. 68, 705–723 (1976)
Simpson, F.O., Devine, C.E.: The fine structure of autonomic neuromuscular contacts in arterioles of sheep renal cortex. J. Anat. (Lond.) 100, 127–137 (1966)
Sitrin, M.D., Bohr, D.F.: Ca and Na interaction in vascular smooth muscle contraction. Amer. J., Physiol. 220, 1124–1128 (1971)
Smith, U., Ryan, J.W., Smith, D.S.: Freeze-etch studies of the plasma membrane of pulmonary endothelial cells. J. Cell Biol. 56, 492–499 (1973)
Staehelin, L.A.: Structure and function of intercellular junctions. Int. Rev. Cytol. 39, 191–283 (1974)
Steinsland, O.S., Furchgott, R.F., Kirpekar, S.M.: Biphasic vasocontriction of the rabbit ear artery. Circulat. Res. 32, 49–58 (1973)
Tani, E., Ikeda, K., Kudo, S., Yamagata, S., Higashi, N., Fujihara, E.: Fenestrated vessels in human hemangioblastoma. J. Neurosurg. 40, 696–705 (1974)
Tomita, T.: Current spread in the smooth muscle of the guinea pig vas deferens. J. Physiol (Lond.) 189, 163–176 (1967)
Uehara, Y., Burnstrock, G.: Demonstration of “gap junction” between smooth muscle cells. J. Cell Biol. 44, 215–217 (1970)
Van Breemen, C., Farinas, B.R., Casteels, R., Gerba, P., Wuytack, F., Deth, R.: Factors controlling Ca+ + concentration. Phil. Trans. B 57–71 (1973)
Verity, M.A., Bevan, J.A.: Fine structural study of the terminal effector plexus, neuromuscular and intermuscular relationships in the pulmonary artery. J. Anal. (Lond.) 103, 49–63 (1968)
Wade, J.B., Karnovsky, M.J.: The structure of the zonula occludens. A single fibril model based on freeze-fracture. J. Cell Biol. 60, 168–180 (1974)
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Tani, E., Yamagata, S. & Ito, Y. Cell membrane structure of vascular smooth muscle of circle of willis. Cell Tissue Res. 179, 131–142 (1977). https://doi.org/10.1007/BF00278468
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DOI: https://doi.org/10.1007/BF00278468