Histoenzymological methods were used to study metabolism of smooth muscle cells of intramural myocardial arteries during experimental aortic or pulmonary artery stenosis. Aortic stenosis was accompanied by changes in smooth muscles of the left ventricle manifested by deceleration of tricarboxylic acid cycle, inhibition of oxidation of free fatty acids and their metabolites, flux redistribution in the glycolytic cascade, and inhibition of shuttle systems and biosynthetic processes. Similar metabolic alterations were observed in vessels of the ventricular septum, but they were not revealed in vessels of the right ventricle (except glycolysis stimulation). Under conditions of pulmonary artery stenosis, histoenzymological alterations in vascular smooth muscle of both ventricles and ventricular septum were similar, which attested to acceleration of tricarboxylic acid cycle, stimulation of oxidation of the free fatty acids with their metabolites, acceleration of glycolysis, and activation of the shuttle systems and biosynthetic processes. Comparative analysis of histoenzymological alterations revealed substantial differences in the character of metabolic changes under conditions of increased left and right ventricular afterload, which can be caused by peculiarities in myocardial blood flow, severity of circulatory disorders, severity of hypoxia, and intensity of processes maintaining ionic homeostasis in vascular smooth muscles and transport across the histohematic barriers. The data attest to important metabolic role of glycolysis in vascular smooth muscles of the myocardium, especially under conditions of enhanced afterload of the right ventricle.
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References
L. A. Krichevskii and B. Kandyukov, Functional status of the right ventricle and its correction during cardiopulmonary bypass surgery. Grudn. Serd.-Sosud. Khir., No. 1, 50-55 (2004).
A. Lehninger, Principles of Biochemistry [Russian translation], Moscow (1985).
J. W. Peterson, Metabolism and Energetics of Vascular Smooth Muscle, Physiology and Pathophysiology of the Heart, Ed. N. Sperelakis [Russian translation], Moscow (1988), Vol. 2, pp. 537-560.
V. Savel’ev, E. G. Yablokov, and A. I. Kirienko, Massive Embolism of Pulmonary Arteries [in Russian], Moscow (2000).
M. S. Tverskaya, O. D. Mishnev, V. V. Karpova, N. S. Izmailova, M. A. Chumakova, A. O. Virganskii, M. Kh. Kadyrova, N. Z. Abdulkerimova, and N. A. Bobrova, Sympathoadrenal system under conditions of increased left and right ventricular afterload. Bull. Exp. Biol. Med., 138, No. 5, 529-531 (2004).
M. S. Tverskaya, V. V. Sukhoparova, V. V. Karpova, A., Raksha, M. Kh. Kadyrova, N. Z. Abdulkerimova, and N. A. Bobrova, Pathomorphology of myocardial circulation: Comparative study in increased left or right ventricle afterload. Bull. Exp. Biol. Med., 145, No. 3, 377-381 (2008).
M. S. Tverskaya, V. V. Sukhoparova, V. V. Karpova, M. Kh. Kadyrova, and V. Yu. Klyuchikov, Histoenzymological Characteristics of the Contractile Myocardium in Experimental Stenosis of the Aorta. Bull. Exp. Biol. Med., 152, No. 1, 108-111 (2011).
M. S. Tverskaya, V. V. Sukhoparova, V. V. Karpova, M. Kh. Kadyrova, and V. Yu. Klyuchikov, Histoenzymology of the Contractile Myocardium in Experimental Pulmonary Stenosis. Bull. Exp. Biol. Med., 154, No. 3, 306-308 (2013).
M. S. Tverskaya, V. V. Sukhoparova, M. Kh. Kadyrova, V. Yu. Klyuchikov, and N. A. Bobrova, Histoenzymological Characteristics of the Heart Conduction System: Comparative Study with Left or Right Ventricle Afterload, Bull. Exp. Biol. Med., 155, No. 5. 618-621 (2013).
M. Forbes, Vascular Smooth Muscle Cells and Other Periendothelial Cells of Mammalian Heart, Physiology and Pathophysiology of the Heart, Ed. N. Sperelakis [Russian translation], Moscow (1988), pp. 365-397.
W. C. Elliott and D. C. Elliott, Biochemistry and Molecular Biology [Russian translation], Moscow (2000).
L. H. Opie, Cardiac metabolism – emergence, decline, and resurgence. Part I. Cardiovas Res., 26, No. 8, 721-733 (1992).
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 161, No. 1, pp. 13-17, January, 2016
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Tverskaya, M.S., Sukhoparova, V.V., Klyuchikov, V.Y. et al. Histoenzymological Characteristics of Smooth Muscle Cells in Myocardial Vessels: A Comparative Study under Conditions of Increased Left or Right Ventricular Afterload. Bull Exp Biol Med 161, 7–10 (2016). https://doi.org/10.1007/s10517-016-3332-3
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DOI: https://doi.org/10.1007/s10517-016-3332-3