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The effect of three rad genes on survival, inter- and intragenic mitotic recombination in Saccharomyces

I. UV irradiation without photoreactivation or liquid-holding post-treatment

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Summary

The effect of UV irradiation on the survival, inter- and intragenic mitotic recombination of 3 diploid UV sensitive Saccharomyces mutants was studied and compared with the wild type RAD. These strains, homozygous for either the RAD, r s1 , rad 9-4, or rad 2-20 gene, have DRF values for survival of 1:1.6:3:20.6 respectively, at LD1. Their recombination behaviour is not correlated to their survival characteristics. The RAD, r s1 , and rad 2-20 strains showed UV induced mitotic inter- and intragenic recombinants; the induction in the r s1 diploid is ca. 100 times greater for both the inter- and intragenic recombinants than in the RAD strain. The rad 9-4 diploid produced no UV induced mitotic recombinants whatsoever, and is therefore considered to be a rec - mutation.

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Authors and Affiliations

  1. Zentralinstitut für Biochemie und Biophysik der Freien Universität Berlin, Berlin, Germany

    Susanne Kowalski & Wolfgang Laskowski

  2. Institut für Gärungsgewerbe, D-1000, Berlin 65

    Susanne Kowalski

Authors
  1. Susanne Kowalski
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  2. Wolfgang Laskowski
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Communicated by F. Kaudewitz

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Kowalski, S., Laskowski, W. The effect of three rad genes on survival, inter- and intragenic mitotic recombination in Saccharomyces . Molec. Gen. Genet. 136, 75–86 (1975). https://doi.org/10.1007/BF00275450

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  • DOI: https://doi.org/10.1007/BF00275450

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