Summary
Human mid-prophase chromosomes exposed to amethopterin, actinomycin D, and bromodeoxyuridine display more than 2000 bands per haploid set and show a remarkable resemblance to the fine chromomeric structures of early pachytene.
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References
Dutrillaux B (1977) New chromosome techniques. In: Yunis JJ (ed) Molecular structure of human chromosomes chromosomes in biology and medicine monograph series. Academic Press, New York, pp 233–265
Hsu TC, Pathak S, Schafer DA (1973) Induction of chromosome crossbanding by treating cells with chemical agents before fixation. Exp Cell Res 79:484–487
Hungerford DA, Hungerford AM (1978) Chromosome structure and function in man. VI. Pachytene chromosome maps of 16, 17, and 18; pachytene as a reference standard for metaphase banding. Cytogenet Cell Genet 21:212–230
ISCN (1978) An international system for human cytogenetic nomenclature (1978). Birth Defects 14, No. 8. The National Foundation, New York
Luciani JM, Devictor M, Morrazzani M-R, Stahl A (1977) Pachytene mapping of the C9 and acrocentric bivalents in the human oocyte. Hum Genet 36:197–204
Muller W, Crothers DM (1968) Studies of the binding of actinomycin and related compounds to DNA. J Mol Biol 35: 251–290
Palmer CG, Funderburk S (1965) Secondary constrictions in human chromosomes. Cytogenet 4:261–276
Paris Conference (1971) Standardization in human cytogenetics. Birth Defects 8, No. 1 (1972). The National Foundation, New York
Pathak S, McGill M, Hsu TC (1975) Actinomycin D effects on mitosis and chromosomes: Sticky chromatids and localized lesions. Chromosoma 50:79–88
Viegas-Pequignot E, Dutrillaux B (1978) Une methode simple pour obtenir des prophases et les prometaphases. Ann Genet (Paris) 21:122–125
Yunis JJ (1976) High resolution human chromosomes. Science 191:1268–1270
Yunis JJ (1980) Nomenclature for high resolution human chromosomes. Cancer Genet and Cytogenet 2:221–229
Yunis JJ, Chandler ME (1977) High-resolution chromosome analysis in clinical medicine. Prog Clin Pathol 7:267–288
Yunis JJ, Sawyer JR, Ball DW (1978) The characterization of high-resolution G-banded chromosomes of man. Chromosoma 67:293–307
Yunis JJ, Ball DW, Sawyer JR (1979) G-banding patterns of high-resolution human chromosomes 6–22, X and Y. Hum Genet 49:291–306
Zakharov AF, Egolina NA (1972) Differential spiralization along mammalian mitotic chromosomes. Chromosoma 38: 341–366
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Supported in part by grant No. 1-568 from the National Foundation and by NIH grant GM26800
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Yunis, J.J. Mid-prophase human chromosomes. The attainment of 2000 bands. Hum Genet 56, 293–298 (1981). https://doi.org/10.1007/BF00274682
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DOI: https://doi.org/10.1007/BF00274682