Summary
Forty-two potato plants were regenerated from a hairy-root line obtained after infection of a shoot of Solanum tuberosum cv ‘Desiree’ with Agrobacterium rhizogenes strain LBA 9402 (pRil855). Transformed plants were uniform and had a distinct phenotype and development compared with untransformed controls. Their growth was vigorous, especially early in their development, their roots were abundant and showed reduced geotropism, their leaves were slightly crinkled and glossy and they produced longer tubers with more frequent, prominent eyes. Cytological examination showed that ten of the forty-two transformed plants had either 47 or 49 chromosomes instead of the normal 48. In two of these aneuploids structural changes were observed.
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References
Ackermann C (1977) Pflanzen aus Agrobacterium rhizogenes Tumoren an Nicotiana tabacum. Plant Sci Lett 8:23–30
Akiyoshi DE, Morris RO, Hunt R, Mischke BS, Kosuge T, Garfinkel DJ, Gordon MP, Nester EW (1983) Cytokinin/auxin balance on crown gall tumors is regulated by specific loci on the T-DNA. Proc Natl Acad Sci USA 80:407–411
Binns AN (1984) The biology and molecular biology of plant cells infected by Agrobacterium tumefaciens. In: Miflin BJ (ed) Oxford surveys of plant molecular and cell biology, vol 1. Oxford University Press, Oxford, pp 133–161
Chilton MD, Drummond MN, Merlo DJ, Sciaky D, Montoya AL, Gordon MP, Nester EW (1977) Stable incorporation of plasmid DNA into higher plant-cells: the molecular basis of crown gall tumorigenesis. Cell 11:263–271
De Block M, Herrera-Estrella L, van Montagu M, Schell J, Zambryski P (1984) Expression of foreign genes in regenerated plants and in their progeny. EMBO J 3:1681–1691
Diener TO (1971) Viroids and viroid diseases. Wiley and Sons, New York, p 10
Garfinkel DJ, Simpson RB, Ream LW, White FF, Gordon MP, Nester EW (1981) Genetic analysis of crown gall: fine structure map of the T-DNA by site directed mutagenesis. Cell 27:143–154
Hooykaas PJJ, Schilperoort RA (1983) The molecular genetics of crown gall tumorigenesis. Adv Genet 22:210–283
Horsch RB, Fraley RT, Rogers SG, Sanders PR, Lloyd A, Hoffmann N (1984) Inheritance of functional foreign genes in plants. Science 223:496–498
Huffman GA, White FF, Gordon MP, Nester EW (1984) Hairy-root-inducing plasmid: physical map and homology to tumor inducing plasmids. J Bacteriol 157:269–276
Karp A, Nelson RS, Thomas E, Bright SWJ (1982) Chromosome variation in protoplast-derived potato plants. Theor Appl Genet 63:265–272
Krens FA, Molendijk L, Wullems GJ, Schilperoort RA (1982) In vitro transformation of plant protoplasts with Ti plasmid DNA. Nature 296:72–74
Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell culture for plant improvement. Theor Appl Genet 60:197–214
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497
Nester EW, Gordon MP, Amasino RM, Yanofski MF (1984) Crown gall — a molecular and physiological analysis. An Rev Plant Physiol 35:387–413
Ooms G, Hooykaas PJJ, Moolenaar G, Schilperoort RA (1981) Crown gall plant tumors of abnormal morphology, induced by Agrobacterium tumefaciens carrying mutated octopine Ti plasmids: analysis of T-DNA functions. Gene 14:33–50
Ooms G, Karp A, Roberts J (1983) From tumour to tuber: tumor cell characteristics and chromosome numbers of crown gall-derived tetraploid potato plants (Solanum tuberosum cv ‘Maris Bard’). Theor Appl Genet 66:169–172
Ooms G, Lenton JR (submitted) T-DNA genes to study plant development: precocious tuberisation and enhanced cytokinins in A. tumefaciens transformed potato
Petit A, David C, Dahl GA, Ellis JG, Guyon P, Casse-Delbart F, Tempe J (1983) Further extension of the octopine concept: plasmids in Agrobacterium rhizogenes cooperate for opine degradation. Mol Gen Genet 190:204–214
Pomponi M, Spano L, Sabbadini MG, Costantino P (1983) Restriction endonuclease map of the root-inducing plasmid of Agrobacterium rhizogenes 1855. Plasmid 10:119–129
Riker AJ, Banfield WM, Wright WH, Keitt GW, Sage HE (1930) Studies on infectious hairy-root of nursery apple trees. J Agric Res 41:507–540
Shepard JF, Bidney D, Shahin E (1980) Potato protoplasts in crop improvement. Science 208:17–24
Smith EF, Townsend CO (1907) A plant-tumor of bacterial origin. Science 25:671–673
Sree Ramulu K, Dijkhuis P, Roest S (1983) Phenotypic variation and ploidy level of plants regenerated from protoplasts of tetraploid potato. Solanum tuberosum L. cv ‘Bintje’. Theor Appl Genet 65:329–338
Tepfer D (1984) Transformation of several species of higher-plants by Agrobacterium rhizogenes — sexual transmission of the transformed genotype and phenotype. Cell 37:959–967
Thomas E, Bright SWJ, Franklin J, Lancaster VA, Miflin BJ, Gibson R (1982) Variation amongst protoplast-derived potato plants (Solanum tuberosum cv ‘Maris Bard’). Theor Appl Genet 62:65–68
Turgeon R, Wood HN, Braun AC (1976) Studies on the recovery of crown gall tumor cells. Proc Natl Acad Sci USA 73:3562–3564
Wheeler VA, Evans NE, Foulger D, Webb KJ, Karp A, Franklin J, Bright SWJ (in press) Shoot formation from explant cultures of fourteen potato cultivars and studies on the cytology and morphology of regenerated plants. Ann Bot
Wullems GJ, Molendijk L, Ooms G, Schilperoort RA (1981) Retention of tumor markers in F1 progeny plants from in vitro induced octopine and nopaline tumor tissues. Cell 4:719–727
White FF, Ghidossi G, Gordon MP, Nester EW (1982) Tumor induction by Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome. Proc Natl Acad Sci USA 79:3193–3197
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Communicated by R. Riley
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Ooms, G., Karp, A., Burrell, M.M. et al. Genetic modification of potato development using Ri T-DNA. Theoret. Appl. Genetics 70, 440–446 (1985). https://doi.org/10.1007/BF00273752
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DOI: https://doi.org/10.1007/BF00273752