Abstract
Six sugarbeet (Beta vulgaris L.) lines (GWI-248, SPB-11, MonoHy 55, SMS-1, EL45 and FC607) were tested for regeneration. Shoot cultures were initiated in vitro from naked, sterilized embryos obtained from mature seed. Excised petioles from cultured shoots were plated on Gamborg's B5 medium and four modified Murashige and Skoog (MS) media. A medium containing MS inorganic salts supplemented with 0.4 mg/1 N6-benzyladenine, 0.1 mg/1 indole-3-butyric acid, ten vitamins and six amino acids, termed RV, was superior for both adventitious shoot and callus formation. Callus was observed only on RV medium and only on petioles that did not develop adventitious buds directly. Rooting of regenerated shoots and development of complete plants was accomplished by transfer to Gamborg's B5 medium with 5 mg/l indole-3-butyric acid as the sole phytohormone. The complete process of regeneration through adventitious shoot production took from 4 to 6 weeks from explants to rooted plants. The callus that formed on nonorganogenic petioles was regenerative when transferred to fresh RV medium. Regeneration from callus occurred mainly by shoot organogenesis but also by somatic embryogenesis at a low frequency.
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Abbreviations
- BA:
-
N6-benzyladenine
- IBA:
-
indole-3-butyric acid
References
DeGreef W, Jacobs, M (1979) Plant Sci. Lett. 17:55–61
Gamborg, OL, Miller RA, Ojima K (1968) Exp. Cell Res. 50:151–158
Hooker MP, Nabors MW (1977) Z. Pflanzenphysiol. 84:237–246
Murashige T, Skoog F (1962) Physiol. Plant 15:473–497
Saunders, JW, Doley WP (1986) J. Plant Physiol 124:473–479
Saunders, JW, Mahoney MD (1981) Euphytica 31:801–804
Saunders, JW, Shin K (1986) Crop Sci. 26:1240–1245
Tetu T, Sangwan RS, Sangwan-Norreel BS (1987) J. Exp. Bot. 38:506–517
Van Geyt JPC, Jacobs M (1985) Plant Cell Rep 4:66–69
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Communicated by G. C. Phillips
Contribution from Missouri Agricultural Experiment Station. Journal Series No. 10394. University of Missouri, Columbia, MO 63873, USA Mention of trade or company name does not constitute a guarantee or warranty of the product by University of Missouri-Columbia or U.S.D.A. Agricultural Research Service and does not imply their approval to the exclusion of other products that may be suitable.
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Freytag, A.H., Anand, S.C., Rao-Arelli, A.P. et al. An improved medium for adventitious shoot formation and callus induction in Beta vulgaris L. in vitro. Plant Cell Reports 7, 30–34 (1988). https://doi.org/10.1007/BF00272972
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DOI: https://doi.org/10.1007/BF00272972