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Production of tropane alkaloids in cultured cells of Hyoscyamus niger

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Abstract

Tests for calluses rich in tropane alkaloids were made with newly induced calluses of Atropa belladonna, Datura stramonium and Hyoscyamus niger. Only calluses of H. niger gave an alkaloid-positive test.

A Hyoscyamus cell line had the highest total alkaloid content of all the calluses screened by the cell-squash alkaloid assay. Both hyoscyamine and scopolamine were identified in the cultured cells of this line by TLC, GLC and GC-MS.

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Abbreviations

NAA:

1-Naphthaleneacetic acid

BA:

Benzyladenine

BSA:

N,O-Bis(trimethylsilyl) acetamide

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Authors and Affiliations

  1. Department of Agricultural Chemistry, Kyoto University, 606, Kyoto, Japan

    Yasuyuki Yamada & Takashi Hashimoto

Authors
  1. Yasuyuki Yamada
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  2. Takashi Hashimoto
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Yamada, Y., Hashimoto, T. Production of tropane alkaloids in cultured cells of Hyoscyamus niger . Plant Cell Reports 1, 101–103 (1982). https://doi.org/10.1007/BF00272363

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  • DOI: https://doi.org/10.1007/BF00272363

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