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X-ray-induced mitotic recombination, gene conversion and mutation in Saccharomyces and the radioprotective effect of cysteine

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Summary

  1. 1.

    The X-ray-induced frequency of mutation, mitotic recombination and gene conversion was determined in Saccharomyces cerevisiae.

  2. 2.

    X-rays induced only few mutations compared with alkylating nitrosamides but were highly efficient in the induction of mitotic recombination and gene conversion. Therefore, X-rays in spite of their low mutagenicity turned out genetically to be a very harmful agent for diploid yeast cells.

  3. 3.

    A protection against X-ray induced recombination and conversion could be achieved by the well known protecting agent cysteine. The protection factor is about 2. A similar effect could be observed with yeast extract-peptone medium but not with the amino acid serine.

  4. 4.

    Furthermore, the protective effect of cysteine and yeast-extract peptone medium was studied at various concentrations and preincubation times.

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  1. Forstbotanisches Institut der Universität Freiburg i. Br., Freiburg i. Br., Germany

    R. Schwaier

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  1. R. Schwaier
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Schwaier, R. X-ray-induced mitotic recombination, gene conversion and mutation in Saccharomyces and the radioprotective effect of cysteine. Molec. Gen. Genet. 101, 203–211 (1968). https://doi.org/10.1007/BF00271622

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  • DOI: https://doi.org/10.1007/BF00271622

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