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Identification and comparative sequence analysis of a gene in equine herpesvirus 1 with homology to the herpes simplex virus glycoprotein D gene

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Abstract

A homologue of the herpes simplex virus (HSV) glycoprotein D gene has been identified in the genome of equine herpesvirus-1 (EHV-1, equine abortion virus). An open reading frame in the middle of the short unique (US) region is capable of encoding a polypeptide of 402 amino acids that has 26% and 20% of its residues matching pseudorabies virus (PRV) gp50 and HSV-1 gD, respectively. Despite this low level of similarity, the positional identity of six cysteine residues and certain motifs, and the location of the EHV-1 gene, clearly define the EHV-1 polypeptide as one of a family of “gD-like” proteins. Two transcripts of 3.3–3.6 kb and 5.4–5.9 kb were identified, consistent with coterminal mRNAs for the EHV-1 gD gene and the adjacent upstream gene, respectively. Partial sequencing of other regions in US also revealed EHV-1 homologues of HSV-1 gE and gI genes, and a possible equivalent gene to PRV gX. By analogy with the ability of HSV-1 gD and PRV gp50 to induce strong anti-viral immune responses, the EHV-1 gD gene product is expected to be an excellent candidate for development as a vaccine antigen.

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References

  1. O'CallaghanD.J., GentryG.A. and RandallC.C., in RoizmanB. (ed.) The Herpesviruses, Plenum Press, New York, 1983, pp. 215–318.

    Google Scholar 

  2. AllenG.P. and BryansJ.T., in PandeyR. (ed.) Progress in Veterinary Microbiology and Immunology. S. Karger, Basel, 1986, pp. 78–144.

    Google Scholar 

  3. CampbellT.M. and StuddertJ., Vet Bull 53, 135–146, 1983.

    Google Scholar 

  4. SpearP.G., in RoizmanB. (ed.) The Herpesviruses. Plenum Press, New York, 1985, pp. 315–356.

    Google Scholar 

  5. McGeochD.J., DalrympleM.A., DavisonA.J., DolanA., FrameC., McNabD., PerryL.J., ScottJ.E. and TaylorP., J Gen Virol 89, 1531–1574, 1988.

    Google Scholar 

  6. TurtinenL.W. and AllenG.P., J Gen Virol 63, 481–485, 1982.

    Google Scholar 

  7. AllenG.P. and YearganM.R., J Virol 61, 2454–2461, 1987.

    Google Scholar 

  8. DavisonA.J. and WilkieN.M., J Gen Virol 64, 1927–1942, 1983.

    Google Scholar 

  9. DavisonA.J. and ScottJ.E., J Gen Virol 67, 1759–1810, 1986.

    Google Scholar 

  10. WhalleyJ.M., RobertsonG.R., ScottN.A., HudsonG.C., BellC.W. and WoodworthL.M., J Gen Virol 70, 383–394, 1989.

    Google Scholar 

  11. AllenG.P. and CoogleL.D., J Virol 62, 2850–2858, 1988.

    Google Scholar 

  12. RobertsonG.R., ScottN.A., MillerJ.M., BellC.W., SabineM., ZhengM., and WhalleyJ.M., DNA Sequence, 1, 241–249, 1991.

    Google Scholar 

  13. StokesA., AllenG.P., PullenL.A. and MurrayP.K., J Gen Virol 70, 1173–1183, 1989.

    Google Scholar 

  14. GuoP.X., GoebelS., DavisS., PerkusM.E., LanguetB., DesmettreP., AllenG. and PaolettiE., J Virol 63, 4189–4198, 1989.

    Google Scholar 

  15. BellC.W., BoyleD.B. and WhalleyJ.M., J Gen Virol 71, 1119–1129, 1990.

    Google Scholar 

  16. McGeochD.J., DolanA., DonaldS. and RixonF.J., J Mol Biol 181, 1–13, 1985.

    Google Scholar 

  17. PetrovskisE.A., TimminsJ.G. and PostL.E., J Virol 60, 185–193, 1986.

    Google Scholar 

  18. CullinaneA.A., RixonF.J. and DavisonA.J., J Gen Virol 69, 1575–1590, 1988.

    Google Scholar 

  19. WatsonR.J., WeisJ.H., SalstromJ.S. and EnquistL.W., J Invest Dermatol 83, 102–111, 1984.

    Google Scholar 

  20. PetrovskisE.A., TimminsJ.G., ArmentroutM.A., MarchioliC.C., YanceyR.J. and PostL.E., J Virol 59, 216–223, 1986.

    Google Scholar 

  21. ReaT.J., TimminsJ.G., LongG.W. and PostL.E., J Virol 54, 21–29, 1985.

    Google Scholar 

  22. ChanW., Immunology 49, 343–352, 1983.

    Google Scholar 

  23. BlacklawsB.A., NashA.A. and DarbyG., J Gen Virol 68, 1103–1114, 1987.

    Google Scholar 

  24. PaolettiE., LipinskasB.R., SamsonoffC., MercerS. and PanicaliD., Proc Natl Scad Sci USA 81, 193–197, 1984.

    Google Scholar 

  25. BlacklawsB.A. and NashA.A., J Gen Virol 71, 863–871, 1990.

    Google Scholar 

  26. KrishnaS., BlacklawsB.A., OvertonH.A., BishopD.H. and NashA.A., J Gen Virol 70, 1805–1814, 1989.

    Google Scholar 

  27. LongD., MadaraT.J., Ponce de LeonM., CohenG.H., MontgomeryP.C. and EisenbergR.J., Infect Immun 43, 761–764, 1984.

    Google Scholar 

  28. BermanP.W., GregoryT., CraseD. and LaskyL.A., Science 227, 1490–1492, 1985.

    Google Scholar 

  29. MarchioliC.C., YanceyR.J., PetrovskisE.A., TimminsJ.G. and PostL.E., J Virol 61, 3977–3982, 1987.

    Google Scholar 

  30. RobertsonG.R. and WhalleyJ.M., in Della-PortaA.J. (ed.) Viruses of Veterinary Importance in Southeast Asia and the Western Pacific. Academic Press, Sydney, 1985, pp. 471–472.

    Google Scholar 

  31. SangerF., NicklenS. and CoulsonA.R., Proc Nat Acad Sci USA 74, 5463–5467, 1977.

    Google Scholar 

  32. BreenE.J., BrowneL.H., GlueL. and WilliamsK.L., Comp Appl Biosci 4, 217, 1988.

    Google Scholar 

  33. SambrookJ., FritschE.F. and ManiatisT., Molecular Cloning—A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.

    Google Scholar 

  34. KozakM., J Mol Biol 196, 947–950, 1987.

    Google Scholar 

  35. EverettR.D., Nucleic Acids Res 11, 6647–6666, 1983.

    Google Scholar 

  36. RixonF.J. and McGeochD.J., Nucleic Acids Res 13, 953–973, 1985.

    Google Scholar 

  37. KornfeldR. and KornfeldS., Ann Rev Biochem 54, 631–664, 1985.

    Google Scholar 

  38. vonHeijneG., Nucleic Acids Res 14, 4683–4690, 1986.

    Google Scholar 

  39. RobertsonG.R. and WhalleyJ.M., Nucleic Acids Res 16, 11303–11317, 1988.

    Google Scholar 

  40. GriffinA.M. and BoursnellM.E.G., J Gen Virol 71, 841–850, 1990.

    Google Scholar 

  41. Brown F., 25, 141–143, 1986.

  42. JohnsonR.M. and SpearP.G., J Virol 63, 819–827, 1989.

    Google Scholar 

  43. LigasM.W. and JohnsonD.C., J Virol 62, 1486–1494, 1988.

    Google Scholar 

  44. LongneckerR. and RoizmanB., J Virol 58, 583–591, 1986.

    Google Scholar 

  45. GompelsU.A., CraxtonM.A. and HonessR.W., J Gen Virol 69, 2819–2829, 1988.

    Google Scholar 

  46. EisenbergR.J., LongD., Ponce de LeonM., MatthewsJ.T., SpearP.G., GibsonM.G., LaskyL.A., BermanP., GolubE. and CohenG.H., J Virol 53, 634–644, 1985.

    Google Scholar 

  47. FullerA.O. and SpearP.G., J Virol 55, 475–482, 1985.

    Google Scholar 

  48. MinsonA.C., HodgmanT.C., DigardP., HancockD.C., BellS.E. and BuckmasterE.A., J Gen Virol 67, 1001–1013, 1986.

    Google Scholar 

  49. WilcoxW.C., LongD., SodoraD.L., EisenbergR.J. and CohenG.H., J Virol 62, 1941–1947, 1988.

    Google Scholar 

  50. CohenG.H., LongD., MatthewsJ.T., MayM. and EisenbergR.J., J Virol 46, 679–689, 1983.

    Google Scholar 

  51. SodoraD.L., CohenG.H. and EisenbergR.J., J Virol 63, 5184–5193, 1989.

    Google Scholar 

  52. Serafini-CessiF., Dall'OlioF., MalagoliniN., PereiraL. and Campadelli-FiumeG., J Gen Virol 69, 869–877, 1988.

    Google Scholar 

  53. StannardL.M., Oveta-FullerA. and SpearP.G., J Gen Virol 68, 715–725, 1987.

    Google Scholar 

  54. ChouP.Y. and FasmanG.D., Annu Rev Biochem 47, 251–276, 1978.

    Google Scholar 

  55. MartinS., CantinE. and RouseB.T., J Gen Virol 70, 1359–1370, 1989.

    Google Scholar 

  56. KrishnaS., BlacklawsB.A., OvertonH.A., BishopD.H. and NashA.A., J Gen Virol 70, 1805–1814, 1989.

    Google Scholar 

  57. WhalleyJ.M., RobertsonG.R. and DavisonA.J., J Gen Virol 57, 307–323, 1981.

    Google Scholar 

  58. KyteJ. and DoolittleR.F., J Mol Biol 157, 105–132, 1982.

    Google Scholar 

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Authors and Affiliations

  1. School of Biological Sciences, Macquarie University, 2109, Sydney, Australia

    Millar Whalley, Graham Robertson, Christopher Bell, Lisa Wiley & Duncan Craven

  2. Department of Veterinary Pathology, University of Sydney, 2006, Australia

    Daria Love & Martin Elphinstone

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  1. Millar Whalley
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  2. Graham Robertson
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  3. Christopher Bell
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  4. Daria Love
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  5. Martin Elphinstone
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  6. Lisa Wiley
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  7. Duncan Craven
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Whalley, M., Robertson, G., Bell, C. et al. Identification and comparative sequence analysis of a gene in equine herpesvirus 1 with homology to the herpes simplex virus glycoprotein D gene. Virus Genes 5, 313–325 (1991). https://doi.org/10.1007/BF00271530

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  • DOI: https://doi.org/10.1007/BF00271530

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