Abstract
An embryogenic callus was obtained from immature panicle of an interspecific hybrid (Oryza sativa x O. latifolia) F1. The medium consisted of HE salts supplemented with 2,4-D, NAA (each 2 mg/l), kinetin (3 mg/l), yeast extract (1360 mg/l) and casein hydrolyzate (300 mg/l). The callus was milk-white in colour compact and granulate in texture. Various developmental stage of embryoid, such as globular, heart-shape, scutellum-shape and mature embryoid were observed in an embryogenic callus. Plantlets were successfully regenerated from 1-month-old callus with more than 80% regenerational frequency in each subculture for 12 passages.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- NAA:
-
naphthaleneacetic acid
- BA:
-
benzyladenine
- IAA:
-
indole acetic acid
- kn:
-
kinetin
- MS:
-
Murashige and Skoog medium (Murashige and Skoog 1962)
- HE:
-
HE5 medium (Ling et al. 1977)
- H:
-
H medium (Nitsch 1969)
- N6 :
-
N6 medium (Zhu et al. 1975)
- B5 :
-
B5 medium (Gamborg et al. 1968)
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Ling, D.H., Chen, W.Y., Chen, M.F. et al. Somatic embryogenesis and plant regeneration in an interspecific hybrid of Oryza . Plant Cell Reports 2, 169–171 (1983). https://doi.org/10.1007/BF00270094
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DOI: https://doi.org/10.1007/BF00270094