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Callus formation from leaf mesophyll protoplasts of Malus Xdomestica Borkh. cv. Greensleeves

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Abstract

Large yields (1.85 × 107/g.f.wt.) of viable protoplasts were obtained from leaves of axenic shoot cultures of Malus Xdomestica Borkh. cv. ‘Greensleeves’. Protoplasts cultured in liquid or agarose semi-solidified KM8P medium underwent cell wall regeneration and colony formation.

Protoplast-derived cell colonies developed to callus on semi-solid KM8 medium. This is the first report of callus formation from mesophyll protoplasts of apple.

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Abbreviations

BAP:

6-benzylaminopurine

K:

kinetin

Z:

zeatin

GA3 :

gibberellic acid

IBA:

3-indole butyric acid

NAA:

1-naphthalene acetic acid

IAA:

3-indole acetic acid

ABA:

abscisic acid

f.wt.:

fresh weight

MS:

Murashige and Skoog (1962)

References

  • Evaldsson I (1985) Swedish J. Agric. Res. 15:119–122

    Google Scholar 

  • Huhtinen O, Honkanen J, Simola LK (1982/83) Plant Sci. Letts. 28:3–9

    Google Scholar 

  • Huang S-C, Millikan DF (1983) Randall DD, Blevins DG, Larson RL and Rapp BJ (eds) Current Topics in Plant Biochemistry and Physiology p249

  • James DJ, Passey AJ, Malhorta SB (1984) East Malling Res. Stn. Rep. 1983 p63

  • Kao KN, Michayluk MR (1975) Planta 126: 105–110

    Google Scholar 

  • Louider M, Hauptmann R, Widholm JM, Skirvin RM, Korban SS (1984) Plant Cell Rep. 3:142–145

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant. 15:473–497

    Google Scholar 

  • Niizeki M, Hidano Y, Saito K-i (1983) Japan J. Breed. 33(4):369–374

    Google Scholar 

  • Ochatt SJ, Caso OH (1986) J. Plant Physiol. 122:243–249

    Google Scholar 

  • Ochatt SJ, Cocking EC, Power JB (1987) Plant Sci. 50:139–143

    Google Scholar 

  • Ochatt SJ, Power JB (1988) Plant Sci. (in press)

  • Oka S, Ohyama K (1985) J. Plant Physiol. 119:455–460

    Google Scholar 

  • Power, JB, Chapman JV, Wilson D (1984) Laboratory Manual: Plant Tissue Culture, Plant Genetic Manipulation Group, University of Nottingham, UK

    Google Scholar 

  • Revilla MA, Ochatt SJ, Doughty S, Power JB (1987) Plant Sci. 50:133–137

    Google Scholar 

  • Russell JA, McCown BH (1986) Plant Sci. 46:133–142

    Google Scholar 

  • Wallin A, Welander M (1985) Plant Cell Tiss. Org. Cult. 5:69–72

    Google Scholar 

  • Wallin A (1987) Proc. 7th Internalt. Protoplast Sympos., Wageningen, Holland p128B

    Google Scholar 

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Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, Nottingham, Great Britain

    S. Doughty & J. B. Power

Authors
  1. S. Doughty
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  2. J. B. Power
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Communicated by I. Potrykus

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Doughty, S., Power, J.B. Callus formation from leaf mesophyll protoplasts of Malus Xdomestica Borkh. cv. Greensleeves. Plant Cell Reports 7, 200–201 (1988). https://doi.org/10.1007/BF00269323

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  • DOI: https://doi.org/10.1007/BF00269323

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