Abstract
Conditions have been established for the optimization of the specific activity of a membrane-bound monoterpene hydroxylase from cell suspension cultures of Catharanthus roseus. In time course studies, the hydroxylase and NADPH-cytochrome c reductase exhibited maximal activities 18–20 days after inoculation, i.e., during early stationary phase. By late stationary phase, enzyme activity had declined. In contrast an enzyme of primary metabolism achieved optimal specific activity by the 12th day and remained constant through day 26, synchronous with general growth. Effects of nutritional and hormonal factors on the specific activity of the hydroxylase and cell growth were evaluated. Inhibitors of hydroxylase activity were also assessed in vitro. A soluble form of the monoterpene hydroxylase has been detected in cultured cells possibly affording a useful source of this enzyme for further purification.
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References
Beers RF Jr, Sizer IW (1952) J. Biol. Chem. 195:133–140
Carew DP (1975) In: Taylor WI, Farnsworth NR (eds) The Catharanthus Alkaloids, Marcel Dekker, Inc. New York, pp 193–208
Carew DP, Krueger RJ (1977) Lloydia 40:326–336
Cordell GA (1974) Lloydia 37:219–298
Courtois D, Guerin J (1980) Plant Science Letters 17:473–482
Courtois D, Kurkdjian A, Guerin J (1980) Plant Science Letters 18:85–96
DeMoss RD (1955) Meth. Enzymol. 1:328–334
Döller G (1978) In. Alfermann AF, Reinhard E (eds) Production of Natural Compounds by Cell Culture Methods, Gesellschaft für Strahlen-und Umweltforschung mbH Munchen pp 109–116
Frear DS, Swanson HR, Tanaka FS (1969) Phytochemistry 8:2157–2169
Gamborg OL (1970) Plant Physiol. 45:372–378
Garnier J, Morel G (1972) Physiol. Veg. 10:617–625
Guenther TM, Kahl GF, Nebert DW (1980) Biochem. Pharmaccl. 29:89–95
Ishimaru A, Yamazaki I (1977) J. Biol. Chem. 252:6118–6124
Knobloch KH, Berlin J (1980) Z. Naturforsch. 35c:551–556
Knobloch KH, Hansen B, Berlin J (1981) Z. Naturforsch. 36c:40–43
Krueger RJ, Carew DP (1978) Lloydia 41:327–331
Kurz WGW, Chatson KB, Constabel F, Kutney JP, Choi LSL, Kolodziejczyk P, Sleigh SK, Stuart KL, Worth BR (1980) Helv. Chim Acta 63:1891–1896
Madyastha KM, Coscia CJ (1979a) In: Swain T, Waller GR (eds) Topics in the Biochemistry of Natural Products, Plenum Press New York, pp 85–129
Madyastha KM, Coscia CJ (1979b) J. Biol. Chem. 254:2419–2427
Madyastha KM, Coscia CJ (1976) Biochemistry 15:1097–1102
Madyastha KM, Ridgway JE, Dwyer JG, Coscia CJ (1977) J. Cell Biol. 72:302–313
McFarlane J, Madyastha KM, Coscia CJ (1975) Biochem. Biophys. Res. Commun. 66:1263–1269
Mizukami H, Nordlov H, Lee SL, Scott AI (1979) Biochemistry 18:3760–3763
Ohnishi TS, Barr JK (1978) Anal. Biochem. 86:193–200
Rich PR, Bendall DS (1975) Eur. J. Biochem. 55:333–341
Scott AI, Mizukami H, Hirata T, Lee SL (1980) Phytochemistry 19:488–489
Shenkman JB, Remmer H, Esterbrook RW (1967) Mol. Pharmacol. 3:113–123
Spitsberg V, Coscia CJ, Krueger RJ (1981) Fed. Proc. 40:1664
Treimer JF, Zenk MH (1979) Eur. J. Biochem. 101:225–233
Young O, Beevers H (1976) Phytochemistry 15:379–385
Zenk MH (1980) J. Nat. Prod. (Lloydia) 43:438–451
Zenk MH, El-Shagi H, Arens H, Stockigt J, Weiler EW, Deus B (1977) In: Barz W, Reinhard E, Zenk MH (eds) Plant Tissue Culture and Its Bio-technological Application, Springer Verlag, Berlin Heidelberg New York, pp 27–43
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Spitsberg, V., Coscia, C.J. & Krueger, R.J. Characterization of a monoterpene hydroxylase from cell suspension cultures of Catharanthus roseus (L.) G. Don. Plant Cell Reports 1, 43–47 (1981). https://doi.org/10.1007/BF00269268
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DOI: https://doi.org/10.1007/BF00269268