Abstract
Suspension cultures of Digitalis lanata were stored at the temperature of liquid nitrogen. After thawing, viable cultures were recovered which showed a growth rate equal to that of untreated cells. The capacity of Digitalis cells to transform β-methyldigitoxin to β-methyldigoxin remained unchanged after cryostorage. This was shown in 300-ml shake cultures as well as in 20-1 bioreactors. From these results it is clear that cryostorage is a suitable method for preserving cell lines with specific capacities to produce compounds of medical and industrial importance.
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Abbreviations
- Mdt:
-
β-methyldigitoxin
- Mdg:
-
β-methyldigoxin
- DMSO:
-
dimethyl sulfoxid
- HPLC:
-
high performance liquid chromatography
- vvm:
-
volume air/volume medium
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Seitz, U., Alfermann, A.W. & Reinhard, E. Stability of biotransformation capacity in Digitalis lanata cell cultures after cryogenic storage. Plant Cell Reports 2, 273–276 (1983). https://doi.org/10.1007/BF00269159
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DOI: https://doi.org/10.1007/BF00269159