Summary
Electrofocusing of carboxymethylated, crystalline Fraction I proteins in polyacrylamide gels containing 8 M urea resolves the large subunit into three major peptides and the small subunit into one or more peptides. Electrofocusing of proteins isolated from leaves of the reciprocal, F1, hybrids: N. glutinosa x N. tabacum, N. glauca x N. tabacum, N. glauca x N. langsdorfii and the parental species confirms that coding information for the large subunit is inherited only by the maternal line whereas both parents contribute coding information for the small subunit. The analysis shows that one or more of the three peptides of the large subunit of Fraction I proteins from different Nicotiana species differ in isoelectric point and therefore serve as phenotypic markers for chloroplast DNA genes.
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Carlson, P. S., Smith, H. H., Dearing, R. D.: Parasexual interspecific plant hybridization. Proc. nat. Acad. Sci. (Wash.) 69, 2292–2294 (1972)
Chan, P. H.: The role of chloroplast and nuclear DNA in coding for the large and small subunits of Fraction I protein isolated from Nicotiana species and reciprocal, interspecific hybrids. Ph.D. Thesis UCLA (1972)
Chan, P. H., Sakano, K., Singh, S., Wildman, S. G.: Crystalline Fraction I protein: Preparation in large yield. Science 176, 1145–1146 (1972)
Chan, P. H., Wildman, S. G.: Chloroplast DNA codes for primary structure of the large subunit of Fraction I protein. Biochim. biophys. Acta (Amst.) 277, 677–680 (1972)
Kawashima, N., Kwok, S.Y., Wildman, S.G.: Studies on Fraction I protein. III. Comparison of the primary structure of the large and small subunits obtained from five species of Nicotiana. Biochim. biophys. Acta (Amst.) 236, 578–586 (1971)
Kawashima, N., Wildman, S. G.: Studies on Fraction I protein. IV. Mode of inheritance of primary structure in relation to whether chloroplast or nuclear DNA contains the code for a chloroplast protein. Biochim. biophys. Acta (Amst.) 262, 42–49 (1972)
Kung, S. D., Sakano, K., Wildman, S. G.: Multiple peptide composition of the large and small subunits of Nicotiana tabacum Fraction I protein ascertained by fingerprinting and electrofocusing. Submitted to Biochim. biophys. Acta (Amst.) (1973)
Sakano, K., Kung, S. D., Wildman, S. G.: Solubility of different crystalline Fraction I proteins in relation to composition of large and small subunits. Submitted to Plant and Cell Phys. (1973)
Singh, S., Wildman, S. G.: Chloroplast DNA codes for the ribulose diphosphate carboxylase catalytic site on Fraction I protein of Nicotiana species. Molec. gen. Genet. 124, 187–196 (1973)
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Communicated by G. Melchers
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Sakano, K., Kung, S.D. & Wildman, S.G. Identification of several chloroplast DNA genes which code for the large subunit of Nicotiana Fraction I proteins. Molec. Gen. Genet. 130, 91–97 (1974). https://doi.org/10.1007/BF00269080
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DOI: https://doi.org/10.1007/BF00269080