Summary
The mechanism of recessive nonsense-suppression was studied in haploid strains of yeast Saccharomyces cerevisiae which were temperature-sensitive due to recessive suppressors. It was demonstrated that these strains carry a defect in protein synthesis leading to the temperature sensitivity of translation. Examination of separate translation events in parent sup - and suppressor-carrying strains has shown that recessive suppression is most likely caused by a mutant protein participating in or affecting the termination of peptide chains on ribosomes.
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References
Algranati, J. D.: Occurrence of termination ribosomes as free 70s particles (E. coli). FEBS Letters 10, 153 (1970)
Ashmarin, I. P., Vasiljev, I. N., Ambrosov, V. A.: Rapid methods of statistical treatment and planning of experiments. [In Russian]. Editions of the Leningrad University (USSR), str. 77 (1971)
Birnboim, H. C.: Method for extraction of ribonucleic acid and polyribosomes from Schizosaccharomyces pombe. J. Bact. 107, 659 (1971)
Breilatt, J., Dickman, S. R.: Detachment of ribosomes from dog pancreas polysomes without concurrent polypeptide synthesis. Biochim. biophys. Acta (Amst.) 195, 531 (1969)
Duell, E. A., Inoue, S., Utter, M. F.: Isolation and properties of intact mitochondria from spheroplasts of yeast. J. Bact. 88, 1762 (1964)
Ganoza, M. C., Vandermeer, J., Debreceni, N., Phillips, S. L.: Mechanism of protein chain termination: Further characterization of a mutant defective in a new protein synthesis factor. Proc. nat. Acad. Sci. (Wash.) 70, 31 (1973)
Gilmore, R. A.: Supersuppressors in saccharomyces cerevisiae. Genetics 56, 641 (1967)
Goodman, H. M., Rich, A.: Mechanism of polyribosome action during protein synthesis. Nature 199, 318 (1963)
Hartwell, L. H., McLaughlin, C. S.: Temperature-sensitive mutants of yeast exhibiting a rapid inhibition of protein synthesis. J. Bact. 96, 1664 (1968)
Hawthorne, D. C.: Identification of nonsense codons in yeast. J. molec. Biol. 43, 71 (1969)
Hirashima, A., Kaji, A.: Factor-dependent breakdown of polysomes. Biochem. biophys. Res. Commun. 41, 877 (1970)
Hirashima, A., Kaji, A.: Factor-dependent release of ribosomes from messenger RNA requirement for two heatstable factors. J. molec. Biol. 65, 43 (1972)
Holley, R. W.: Large-scale preparation of yeast “soluble” ribonucleic acid. Biochem. biophys. Res. Commun. 10, 186 (1963)
Hutchinson, H. T., Hartwell, L. H.: Macromolecule synthesis in yeast spheroplasts. J. Bact. 94, 1697 (1967)
Inge-Vechtomov, S. G.: Identification of some coupling groups in Petergovsky genetical lines of yeast. [In Russian.] Genetika (USSR) 7, 113 (1971)
Inge-Vechtomov, S. G., Andrianova, V. M.: Recessivnie super-supressori u droggei. Genetika (USSR) 6, 103 (1970)
Inge-Vechtomov, S. G., Andrianova, V. M.: New type of suppressors in yeast. [In Russian.] In the collection “Molecular mechanisms of genetic processes”. Nauka (USSR) 189 (1972)
Inge-Vechtomov, S. G., Andrianova, V. M., Mironova, L. N.: Temperature-sensitivity in connection with a semidominant suppressor mutation in yeast. [In Russian.] Bulletin of the Leningrad University (USSR) (1973)
Inge-Vechtomov, S. G., Simarov, B. V.: Connection between suppression and interallelic complementation in the loci ad2 in yeast. [In Russian.] In the collection “Research in genetics”, (USSR) 3, 127 (1967)
Lin, S.-Y., Mosteller, R. D., Hardesty, B.: The mechanism of sodium fluoride and cycloheximide inhibition of hemoglobin biosynthesis in the cell-free reticulocyte system. J. molec. Biol. 21 51 (1966)
Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265 (1951)
Magni, G. E., von Borstel, R. C., Steinberg, C. M.: Supersuppressors as addition-deletion mutations. J. molec. Biol. 16, 568 (1966)
Manney, T. R.: Tryptophan synthetase mutants of yeast: action of supersuppressors in relation to allelic mapping and complementation. Ph. D. Thesis, University of California, p. 119 (1964)
Manney, T. R.: Evidence for chain termination by supersuppressible mutants of yeast. Genetics 60, 719 (1968)
Marcus, L., Ris, H., Halvorson, H. O., Bretthauer, R. K., Bock, R. M.: Occurence, isolation and characterization of polyribosomes in yeast. J. Cell Biol. 34, 505 (1967)
Modolell, J., Davis, B. D. P.: Rapid inhibition of polypeptide chain extension by streptomycin. Proc. nat. Acad. Sci. (Wash.) 61, 1279 (1968)
Mortimer, R. K.: Genetic redudancy in yeast. Genetics 61, 329 (1969)
Mortimer, R. K., Hawthorne, D. C.: Genetic mapping of nonsense suppressors in yeast. Genetics 60, 735 (1968)
Phillips, S. L.: Termination of messenger RNA translation in a temperature-sensitive mutant of Escherichia coli. J. molec. Biol. 59, 461 (1971)
Phillips, S. L., Schlessinger, D., Apirion, D.: Temperature-dependent suppression of UGA and UAA codons in a temperature-sensitive mutant of Escherichia coli. Cold Spr. Harb. Symp. quant. Biol. 34, 499 (1969)
Reeves, R. H., Roth, J. R.: a recessive UGA Suppressor. J. molec. Biol. 56, 523 (1971)
Richter, D., Klink, F.: Amino acid transfer factors from yeast. Isolation and properties of three different transfer-active protein fractions. Biochemistry 6,3569 (1967)
Sherman, F., Stewart, J. W., Parker, J. H., Putterman, G. J., Agraval, B. B. L., Margoliash, E.: The relationship of gene structure and protein structure of iso-1-cytochrom c from yeast. Symp. Soc. exp. Biol. 24, 85 (1970)
Stewart, J. W., Sherman, F.: Demonstration of UAG nonsense codon in bakers yeast by amino acid replacements in iso-1-cytochrom c. J. molec. Biol. 68, 429 (1972)
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Communicated by D. Goldfarb
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Smirnov, V.N., Kreier, V.G., Lizlova, L.V. et al. Recessive super-suppression in yeast. Molec. Gen. Genet. 129, 105–121 (1974). https://doi.org/10.1007/BF00268625
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DOI: https://doi.org/10.1007/BF00268625