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Culture of soybean mesophyll protoplasts in alginate beads

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Abstract

Mesophyll protoplasts were isolated from leaves of 10 day old aseptically grown soybean seedlings, or from surface disinfested leaves of 3 week old plants grown in environmental chambers. The protoplasts were encapsulated in 2mm diameter Ca alginate beads. Immobilized protoplasts were induced to divide by culturing in shaker flasks containing an actively growing soybean cell suspension. The feeder cell suspension supported the division of protoplasts independent of the protoplast density in the Ca alginate beads. At day 18 after encapsulation, the alginate matrix was dissolved, releasing viable callus colonies. The feeder cell suspension obviated plating of protoplasts at high density which is usually required for subsequent cell division and colony development. Since the protoplasts were embedded at low density, the cell colonies were derived from single cells.

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References

  • Adams TL, Townsend JA (1983) Plant Cell Reports 2: 165–168

    Google Scholar 

  • Brodelius P, Deus B, Moshash K, Zenk MH (1979) Swedish Patent Application 79 05615-6

    Google Scholar 

  • Franceschi VR, Maurice SB, Ku SB, Wittenbach VA (1984) Plant Science Letters 36: 181–186

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50: 151–158

    Google Scholar 

  • Gamborg OL, Davis BP, Stahihut RW (1983) Plant Cell Reports 2: 213–215

    Google Scholar 

  • Horsch RB, Jones GE (1980) In vitro 16: 103–108

    Google Scholar 

  • Linsefors L, Brodelius P (1985) Plant Cell Reports 4: 23–27

    Google Scholar 

  • Lorz H, Larkin PJ, Thomson J, Scowcroft WR (1983) Plant Cell Tissue Organ Culture 2: 217–226

    Google Scholar 

  • Schenk RU, Hildebrandt AC (1972), Can J Bot 50: 199–204

    Google Scholar 

  • Scheurich P, Schnabl H, Zimmerman U, Klein J (1980) Biochim Biophys Acta 598: 645–651

    Google Scholar 

  • Schnabl H, Scheurich P, Zimmerman U (1980) Planta 149: 280–282

    Google Scholar 

  • Shillito RD, Paszkowski J, Potrykus I (1983) Plant Cell Reports 2: 244–247

    Google Scholar 

  • Zieg RG, Outka DE (1980) Plant Science Letters 18: 105–114

    Google Scholar 

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Author notes

  1. David M. Tricoli

    Present address: Upjohn Company, 49002, Kalamazoo, MI, USA

  2. Mich B. Hein

    Present address: Research Institute of Scripps Clinic, MB3, 10666N, Torrey Pines Rd., 92097, La Jolla, CA, USA

Authors and Affiliations

  1. Monsanto Agricultural Company, 700 Chesterfield Village Parkway, 63198, Chesterfield, MO, USA

    David M. Tricoli, Mich B. Hein & Michael G. Carnes

Authors
  1. David M. Tricoli
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  2. Mich B. Hein
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  3. Michael G. Carnes
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Communicated by I.K. Vasil

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Tricoli, D.M., Hein, M.B. & Carnes, M.G. Culture of soybean mesophyll protoplasts in alginate beads. Plant Cell Reports 5, 334–337 (1986). https://doi.org/10.1007/BF00268595

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  • DOI: https://doi.org/10.1007/BF00268595

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