Abstract
Maize anthers have been induced on modified N6 medium to produce embryoids. Different stages from the cultures were sampled and prepared for microscopical examination. The microspores at the onset of culture were in an early developmental stage, with the nucleus and numerous organelles centred in the middle, surrounded by many small vacuoles with a lipid content. The binuclear pollen grains contained small vesicles and much starch. The partially condensed vegetative nucleus indicated participation of the vegetative component in the formation of multicellular pollen grains (MPGs). Several MPGs have been observed which differed in morphology. We suggest, on the basis of these ultrastructural observations, that in maize mainly the vegetative cell contributes to the MPG which further develops directly into embryoids.
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References
Anonymous (1975) 401 Research Group, Acta Genet. Sin. 2: 138–142.
Brettell RIS, Thomas E, Wernicke W (1981) Maydica 26: 101–111.
Chu CC (1978) In: Hu H (ed) Proc. Symp. on Plant Tissue Culture Peking, Pitman Publ. Ltd, London: 43–50.
Clapham DH (1977) In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue and organ culture, Springer Verlag, Berlin: 279–298.
Dunwell JM, Sunderland N (1975) J. Exp. Bot. 26: 240–252.
Genovesi AD, Collins GB (1982) Crop Sci. 22: 1137–1144.
Genovesi AD, Magill CW (1982) Plant Cell Rep. 1: 257–260.
Heberle-Bors E (1985) Theor. Appl. Genet. 71: 361–374.
Hu H, Yang H (1986) Haploids of higher plants in vitro, Springer Verlag, Berlin.
Huang B (1986) In: Hu H, Yang H (eds) Haploids of higher plants in vitro, Springer Verlag, Berlin: 91–117.
Janos P (1985) Cereal Res. Comm. 13: 47–53.
Ku MK, Cheng WC, Kuo LG, Kuan YL, An HP, Huang CH (1978) In: Hu H (ed) Proc. Symp. on Plant Tissue Culture Peking, Pitman Publ. Ltd, London: 35–42.
Kuo CS (1978) Acta Bot. Sin. 20: 204–209.
Maheswari SC, Rashid A, Tyagi AK (1982) Am. J. Bot. 69: 865–879.
Miao SH, Kuo CS, Kwei YL, Sun AT, Ku SY, Lu WL, Wang YY, Chen ML, Wu MK, Hang L (1978) In: Hu H (ed) Proc. Symp. on Plant Tissue Culture Peking, Pitman Publ. Ltd, London: 23–33.
Nitsch C, Andersen S, Godard M, Neuffer MG, Sheridan WF (1982) In: Earle ED, Demarly Y (eds) Variability in plants regenerated from tissue culture. Praeger Sci Publ. N.Y.: 69–91.
O'Brien TP, McCully ME (1981) The study of plant structure. Principles and selected methods, Termarcarphi Pty. Ltd, Melbourne.
Petolino JF, Jones AM (1986) Crop Sci. 26: 1072–1074.
Raghavan V (1986) Embryogenesis in angiosperms, Cambridge University Press, Cambridge: 152–189.
Schel JHN, Kieft H, Van Lammeren AAM (1984) Can. J. Bot. 62: 2842–2853.
Sunderland N, Roberts M, Evans LJ, Wildon DC (1979) J. Exp. Bot. 30: 1133–1144.
Ting, YC, Yu M, Zheng WZ (1981) Plant Sci. Lett. 23: 139–145.
Tsay HS, Miao SH, Widholm JM (1986a) J. Plant Physiol. 126: 33–40.
Tsay SS, Tsay HS, Chao CY (1986b) Plant Cell Rep. 5: 119–123.
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Barnabas, B., Fransz, P.F. & Schel, J.H.N. Ultrastructural studies on pollen embryogenesis in maize (Zea mays L.). Plant Cell Reports 6, 212–215 (1987). https://doi.org/10.1007/BF00268482
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DOI: https://doi.org/10.1007/BF00268482