Abstract
Attempts were made to immobilize digitoxin 12β-hydroxylase, a membrane-bound, cytochrome P-450-dependent monooxygenase from cell cultures of Digitalis lanata. The optimum procedure was the entrapment of microsomes in 2% alginate by crosslinking the polysaccharide chains with CaCl2. After the immobilization of the enzyme about 70% of its activity was retained. The kinetic data such as the pH optimum and the optimum substrate concentrations were identical for the immobilized enzyme and freely suspended microsomes. Using β-methyldigitoxin as a substrate enzyme activity could be observed for more than 20 h. A continuous flow system for immobilized digitoxin 12β-hydroxylase is described.
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Abbreviations
- β-mdg:
-
β-methyldigoxin
- β-mdt:
-
β-methyldigitoxin
References
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Communicated by W. Barz
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Petersen, M., Alfermann, A.W., Reinhard, E. et al. Immobilization of digitoxin 12β-hydroxylase, a cytochrome P-450-dependent enzyme from cell cultures of Digitalis lanata EHRH. Plant Cell Reports 6, 200–203 (1987). https://doi.org/10.1007/BF00268479
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DOI: https://doi.org/10.1007/BF00268479