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Isolation of the mini insertions IS6 and IS7 of E. coli

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Summary

The mini IS elements IS6 and IS7 have been detected in constitutive gal + revertants of galOP-308::IS2 (I), in which the expression of the gal operon is turned off by IS2 in orientation I. Both, IS6 and IS7, are integrated into IS2 proximal to the gal structural genes. IS6 is 115 base pairs long and causes 50% constitutive expression of the gal genes. IS7 is only 65 base pairs long and the gal operon is expressed 20% constitutively compared to the gal + wild type operon. Both IS6 and IS7 are excised frequently, in the absence of selective pressure. These findings are discussed with respect to the evolution of gene expression.

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Authors and Affiliations

  1. Institut für Biologie III, Universität Freiburg, Schänzlestr. 1, D-7800, Freiburg, Federal Republic of Germany

    Debabrota Ghosal & Heinz Saedler

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  1. Debabrota Ghosal
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  2. Heinz Saedler
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Communicated by W. Arber

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Ghosal, D., Saedler, H. Isolation of the mini insertions IS6 and IS7 of E. coli . Molec. Gen. Genet. 158, 123–128 (1977). https://doi.org/10.1007/BF00268304

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  • DOI: https://doi.org/10.1007/BF00268304

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