Summary
Summary
Yeast cultures progressing from the exponential to the stationary phase of growth showed changes in cell sensitivity to physical agents such as UV light, heat shock at 52° C and the chemical mutagens ethyl methane sulphonate, nitrous acid and mitomycin C.
Exponential phase cells showed maximum resistance to UV light and minimum resistance to heat shock and the three chemicals. The increased resistance of exponential phase cells to UV light was shown to be dependent upon the functional integrity of the RAD 50 gene.
Treatment of growing yeast cultures with radioactively labelled ethyl methane sulphonate indicated the preferential uptake of radioactivity during the sensitive exponential stage of growth. The results indicated that the differential uptake of the chemical mutagens was responsible for at least a fraction of the variations in cell sensitivity observed in yeast cultures at different phases of growth.
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References
Barranco, S.C., Humphrey, R.M.: The response of Chinese hamster cells to n-methyl-n'nitro-n-nitrosoguanidine. Mutation Res. 11, 421–429 (1971)
Beam, C.A., Mortimer, R.K., Wolf, R.G., Tobias, C.A.: The relation of radioresistance to budding in Saccharomyces cerevisiae. Arch. Biochem. Biophys. 49, 110–122 (1954)
Chanet, R., Waters, R., Moustacchi, E.: The induction of pyrimidine dimers in nuclear DNA after UV irradiation during the synchronous cycle of Saccharomyces cerevisiae. Int. J. Radiat. Biol. 27, 481–485 (1975)
Chanet, R., Williamson, D.H., Moustacchi, E.: Cyclic variation in petite mutagenesis induced by UV light in synchronous yeast strains. Biochem. biophys. Acta (Amst.) 324, 290–299 (1973)
Cox, B.S., Bevan, E.A.: Aneuploidy in yeast. New Phytologist 61, 342–355 (1962)
Cox, B.S., Parry, J.M.: The isolation, genetics and survival characterisites of ultraviolet-light sensitive mutants in yeast. Mutation Res. 6, 37–55 (1968)
Davies, P.J., Evans, W.E., Parry, J.M.: Mitotic recombination induced by chemical and physical agents in the yeast Saccharomyces cerevisiae. Mutation Res. 29, 301–314 (1975)
Deutch, C.E., Parry, J.M.: Sphaeroplast formation in yeast during the transition from exponential to stationary phase. J. gen. Microbiol. 80, 259–268 (1974)
Djordjevic, B., Tolmach, L.H.: Responses of Synchronous populations of Hela cells to ultraviolet irradiation at selectd stages of the generation cycle. Radiat. Res. 32, 327–346 (1967)
Elkind, M.M., Kano, E., Sutton-Gilbert, H.: Cell killing by actinomycin D in relation to the growth cycle of Chinese hamster cells. J. Cell Biol. 42, 366–377 (1969)
Evans, W.E.: Studies upon the inactivation and repair of cellular damage induced by chemical and physical agents. Ph.D. Thesis University of Wales (1974)
Fabre, F.: UV sensitivity of the wild type and different UVS mutants of Schizosaccharomyces pombe: Influence of growth stages and DNA content of the cells. Mutation Res. 10, 415–426 (1970)
Han, A., Sinclair, W.K.: Sensitivity of synchronised Chinese hamster cells to ultraviolet light. Biophys. J. 9, 1171–1192 (1969)
Howell-Saxton, E., Zamenhoff, S., Zamenhoff, P.J.: Response of E. coli to ethylmethane sulphonate: Influence of growth phase and repair ability upon survival and mutagenesis. Mutation Res. 20, 327–337 (1973)
Howell-Saxton, E., Smith, D.C., Zamenhoff, P.J., Zamenhoff, S.: Effects of growth phase and repair capacity on the rejoining of ethylmethane sulphonate induced DNA strand breaks in Escherichi coli Mutation Res. 24, 227–237 (1974)
Parry, J.M., Parry, E.M.: The effects of UV light post-treatment on the survival characteristics of 21 UV-sensitive mutants of the yeast Saccharomyces cerevisiae. Mutation Res. 8, 545–556 (1969)
Pepper, T.: Cyclic X-ray resistance to lethal and non-lethal damage in Saccharomyces cerevisiae. Radiat. Res. 63, 97–118 (1975)
Rauth, A.M., Wilmore, G.F.: The survival of synchronous mouse L cells after UV irradiation. Radiat. Res. 28, 84–95 (1966)
Schenberg-Frasino, A., Moustacchi, E.: Lethal and mutagenic effects of elevated temperature on haploid yeast 1. Variations in sensitivity during the cell cycle. Molec. gen. Genet. 115, 243–257 (1972)
Sinclair, W.K.: Hydroxyurea: Differential lethal effects on cultured mammalian cells during the cell cycle. Science 150, 1729–1731 (1965)
Sinclair, W.K.: Cyclic responses in mammalian cells in vitro. Radiat. Res. 33, 620–643 (1968)
Terisma, T., Tolmach, L.H.: Change in X-ray sensitivities of Hela cells during the division cycle. Nature 190, 1210–1211 (1961)
Tyrrell, R.M., Moss, S.H., Davies, D.J.G.: The variation in UV sensitivity of four K-12 strains of E. coli as a function of their stage of growth. Mutation Res 16, 1–12 (1972)
Unrau, P., Wheatcroft, R., Cox, B.S.: The excision of pyrimidine dimers from DNA of UV irradiated yeast. Molec. gen. Genet. 113, 359–362 (1971)
Waters, R.: Studies on the mechanism of repair of radiation damage in micro-organisms. Ph.D. Thesis University of Wales (1972)
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Parry, J.M., Davies, P.J. & Evans, W.E. The effects of “Cell age” upon the lethal effects of physical and chemical mutagens in the yeast, Saccharomyces cerevisiae . Molec. Gen. Genet. 146, 27–35 (1976). https://doi.org/10.1007/BF00267979
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DOI: https://doi.org/10.1007/BF00267979