Skip to main content
SpringerLink
Log in

Functional homology of fla genes between Salmonella typhimurium and Escherichia coli

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Genetic studies have shown the presence of more than 20 fla genes indispensable for the formation of flagella in Salmonella typhimurium and Escherichia coli. Functional homology of the fla genes in these two bacterial species was examined through intergeneric complementation tests by bacteriophage Pl-mediated transduction from E. coli donors to S. typhimurium recipients. It was found that most of the fla gene products in these two bacterial species were interchangeable and the following correspondence was established (S. typhimurium genes vs. E. coli genes): flaFIV to flaV; flaFV to flaK; flaFVII to flaL; flaFIX to flaM; flaC to flaH; flaM to flaG; flaE to flaI; flaAI to flaN; flaAII·1 to flaB; flaAIII to flaC; flaS to flaO; flaR to flaE; flaQ to flaA; and flaB to flaR. These results suggest that the chromosomal alignment of the functionally homologous genes is very similar in these two bacterial species. Furthermore, five additional fla genes were inferred to exist in E. coli in addition to the fla genes already identified. They were termed flaU, flaX, flaY, flaZ, and flbB (flb is equivalent to fla), which corresponded to flaFI, flaFVI, flaFVIII, flaFX, and flaK of Salmonella in this order. The flaK mutants of E. coli showed no complementation with any of the flaFV, flaFVI, flaFVII, flaFVIII, or flaFIX mutants of Salmonella.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Armstrong, J.B., Adler, J.: Location of genes for motility and chemotaxis on the Escherichia coli genetic map. J. Bacteriol. 97, 156–161 (1969)

    Google Scholar 

  • collins, A.I.T.: Genetic studies on generally nonchemotactic mutants of Salmonella typhimurium. Ph.D. thesis. Stanford Univ., Stanford, Calif. 263 pp. (1974)

  • Colson, C., Van Pel, A.: DNA restriction and modification systems in Salmonella. I. SA and SB, two Salmonella typhimurium systems determined by genes with a chromosomal location compatible to that of the Escherichia coli hsd genes. Mol. Gen. Genet. 129, 325–337 (1974)

    Google Scholar 

  • DeFranco, A.L., Parkinson, J.S., Koshland, D.E., Jr.: Functional homology of chemotaxis genes in Escherichia coli and Salmonella typhimurium. J. Bacteriol. 139, 107–114 (1979)

    Google Scholar 

  • Enomoto, M., Stocker, B.A.D.: Transduction by phage P1kc in Salmonella typhimurium. Virology 60, 503–514 (1974)

    Google Scholar 

  • Iino, T.: Genetics and chemistry of bacterial flagella. Bacteriol. Rev. 33, 454–475 (1969)

    Google Scholar 

  • Iino, T.: Genetics of structure and function of bacterial flagella. Annu. Rev. Genet. 11, 161–182 (1977)

    Google Scholar 

  • Iino, T., Enomoto, M.: Genetic studies of non-flagellate mutants of Salmonella. J. Gen. Microbiol. 43, 315–327 (1966)

    Google Scholar 

  • Joys, T.M., Stocker, B.A.D.: Complementation of non-flagellate Salmonella mutants. J. Gen. Microbiol. 41, 47–55 (1965)

    Google Scholar 

  • Kleckner, N., Chan, R.K., Tye, B.-K., Botstein, D.: Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J. Mol. Biol. 97, 561–575 (1975)

    Google Scholar 

  • Komeda, Y., Silverman, M., Matsumura, P., simon, M.: Genes for the hook-basal body proteins of the flagellar apparatus in Escherichia coli. J. Bacteriol. 134, 655–667 (1978a)

    Google Scholar 

  • Komeda, Y., Silverman, M., Simon, M.: Genetic analysis of Escherichia coli K12 region I flagellar mutants. J. Bacteriol. 131, 801–808 (1977)

    Google Scholar 

  • Komeda, Y., Silverman, M., Simon, M.: Identification of the structural gene for hook subunit protein of Escherichia coli flagella. J. Bacteriol. 133, 364–371 (1978b)

    Google Scholar 

  • Komeda, Y., Suzuki, H., Ishidsu, J., Iino, T.: The role of cAMP in flagellation of Samonella typhimurium. Mol. Gen. Genet. 142, 289–298 (1975)

    Google Scholar 

  • Kutsukake, K., Suzuki, T., Yamaguchi, S., Iino, T.: Role of gene flaFV on flagellar hook formation in Salmonella typhimurium. J. Bacteriol. 140, 267–275 (1979)

    Google Scholar 

  • Lederberg, J.: Linear inheritance in transductional clones. Genetics 41, 743–757 (1956)

    Google Scholar 

  • Lennox, E.S.: Transduction of linked genetic characters of the host by bacteriophage Pl. Virology 1, 190–206 (1955)

    Google Scholar 

  • Mäkelä, P.H.: Genetic homologies between flagellar antigens of Escherichia coli and Salmonella abony. J. Gen. Microbiol. 35, 503–510 (1964)

    Google Scholar 

  • Noel, K.D., Ames, G.F.-L.: Evidence for a common mechanism for the insertion of the Tn10 transposon and for the generation of Tn10-stimulated deletions. Mol. Gen. Genet. 166, 217–223 (1978)

    Google Scholar 

  • Ornellas, E.P., Stocker, B.A.D.: Relation of lipopolysaccharide character to Pl sensitivity in Salmonella typhimurium. Virology 60, 491–502 (1974)

    Google Scholar 

  • Patterson-Delafield, J., Martinez, R.J., Stocker, B.A.D., Yamaguchi, S.: A new fla gene in Salmonella typhimurium-flaR-and its mutant phenotype-superhooks. Arch. Microbiol. 90, 107–120 (1973)

    Google Scholar 

  • Silverman, M., Simon, M.: Flagellar assembly mutants in Escherichia coli. J. Bacteriol. 112, 986–993 (1972)

    Google Scholar 

  • Silverman, M., Simon, M.: Genetic analysis of flagellar mutants in Escherichia coli. J. Bacteriol. 113, 105–113 (1973a)

    Google Scholar 

  • Silverman, M., Simon, M.: Genetic analysis of bacteriophage Mu-induced flagellar mutants in Escherichia coli. J. Bacteriol. 116, 114–122 (1973b)

    Google Scholar 

  • Silverman, M., Simon, M.: Characterization of Escherichia coli flagellar mutants that are insensitive to catabolite repression. J. Bacteriol. 120, 1196–1203 (1974)

    Google Scholar 

  • Silverman, M., Simon, M.: Bacterial flagella. Annu. Rev. Microbiol. 31, 397–419 (1977)

    Google Scholar 

  • Smith, H.O., Levine, M.: A phage P22 gene controlling integration of prophage. Virology 31, 207–216 (1967)

    Google Scholar 

  • Stocker, B.A.D.: Abortive transduction of motility in Salmonella; a nonreplicated gene transmitted through many generations to a single descendant. J. Gen. Microbiol. 15, 575–598 (1956)

    Google Scholar 

  • Suzuki, T., Iino, T., Horiguchi, T., Yamaguchi, S.: Incomplete flagellar structures in nonflagellate mutants of Salmonella typhimurium. J. Bacteriol. 133, 904–915 (1978)

    Google Scholar 

  • Yamaguchi, S., Iino, T., Horiguchi, T., Ohta, K.: Genetic analysis of fla and mot cistrons closely linked to H1 in Salmonella abortusequi and its derivatives. J. Gen. Microbiol. 70, 59–75 (1972)

    Google Scholar 

  • Yokota, T., Gots, J.: Requirement of adenosine 3′, 5′-cyclic monophosphate for flagellar formation in Escherichia coli and Salmonella typhimurium. J. Bacteriol. 103, 513–516 (1970)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Genetics, Department of Biology, Faculty of Science, University of Tokyo, 113, Hongo, Tokyo, Japan

    Kazuhiro Kutsukake & Tetsuo Iino

  2. National Institute of Genetics, 411, Mishima, Shizuoka-ken, Japan

    Yoshibumi Komeda

  3. Department of Biology, School of Education, Waseda University, Nishiwaseda, Shinjuku-ku, 160, Tokyo, Japan

    Shigeru Yamaguchi

Authors
  1. Kazuhiro Kutsukake
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tetsuo Iino
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yoshibumi Komeda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shigeru Yamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by K. Isono

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kutsukake, K., Iino, T., Komeda, Y. et al. Functional homology of fla genes between Salmonella typhimurium and Escherichia coli . Molec. Gen. Genet. 178, 59–67 (1980). https://doi.org/10.1007/BF00267213

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00267213

Keywords

Search

Navigation