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The ribosomal proteins of Drosophila melanogaster

III. Further studies on the ribosomal protein composition during development

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Summary

Ribosomes from mid- and late-third instar larvae, prepupae, 24-hours old pupae, and adult flies were isolated and washed with 0.5 M NH4Cl, 1mM Mg++. The ribosomal proteins were extracted and analyzed by two-dimensional polyacrylamide gel electrophoresis. Defined differences are shown between the protein patterns of whole ribosomes from mid-third instar larvae on one hand and prepupae, 24-hours old pupae, and imagos on the other hand. Late-third instar larval ribosomes exhibit an intermediate protein pattern suggesting a transitory stage with both unchanged and changed ribosomes in larvae ready to pupate. Apart form 1–3 major proteins the alterations comprise medium and minor spots. It cannot be excluded, however, that changes involving weak spots may be attributed to supernatant proteins, which, despite the high salt wash, bind strongly to the ribosomes during preparation. The number of proteins of whole ribosomes from D. melanogaster is estimated to be 68–69.

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Authors and Affiliations

  1. Department of Genetics, University of Umeå, Umeå, Sweden

    Andrew G. Lambertsson

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  1. Andrew G. Lambertsson
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Communicated by H. G. Wittmann

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Lambertsson, A.G. The ribosomal proteins of Drosophila melanogaster . Molec. Gen. Genet. 128, 241–247 (1974). https://doi.org/10.1007/BF00267113

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  • DOI: https://doi.org/10.1007/BF00267113

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