Summary
Three rice (Oryza sativa L.) mutants resistant to hydroxy-L-proline (Hyp), HYP 101, HYP 202 and HYP 203, were selected from an ethylene imine mutagenized M2 population of the original variety, ‘Nipponbare’, and their biochemical and genetical characteristics were investigated. The sensitivity of the mutants to Hyp could be clearly differentiated from that of the original variety when seeds were germinated and cultured with 10−4∼10−3 M Hyp for 10 days. A difference in Hyp sensitivity was also observed among the HYP mutant lines, HYP 101 being the most resistant line. When free amino acids in seeds and 15-day-old seedlings were analyzed, the composition of the amino acids in the mutants was somewhat different from that found in the original variety. However, free proline accumulation was not detected in either the HYP mutants or the original variety. In each mutant line, HYP resistance was transmitted with a single recessive nuclear gene (hpr). These results suggest that the mechanism of Hyp resistance controlled by the recessive gene do not involve free proline accumulation.
Similar content being viewed by others
Abbreviations
- Hyp:
-
hydroxy-L-proline
- T-Pro:
-
thioproline
References
Bright SWJ, Featherstone LC, Miflin BJ (1979a) Lysine metabolism in a barley mutant resistant to S-(2-aminoethyl)cysteine. Planta 146:629–633
Bright SWJ, Norbury PB, Miflin BJ (1979b) Isolation of a recessive barley mutant resistant to S-(2-amino ethyl)-L-cysteine. Theor Appl Genet 55:1–4
Brock RD, Langridge J (1975) Prospects for genetic improvement of seeds protein in plants. In: Breeding for seed protein improvement using nuclear techniques. IAEA, Vienna, pp 3–13
Gengenbach B (1984) Tissue culture and related approaches for grain quality improvement. In: Collins GB, Petolino JG (eds) Applications of genetic engineering to crop improvement. Nijhoff/Junk Publishers, Dordrecht, Boston Lancaster, pp 211–254
Green CE, Phillips RL (1974) Potential selection system for mutants with increased lysine, threonine, and methionine in cereal crops. Crop Sci 14:827–830
Hasegawa H, Inoue M (1983) Induction and selection of hydroxy-L-proline resistant mutant in rice (Oryza sativa L.). Jpn J Breed 33:275–282
Hasegawa H, Mori S, Inoue M, Murakami M (1985) Hydroxy-L-proline resistant mutants in rice. Rice Genet Newslett 2:88–89
Hermann M, Thevenet NJ, Coudert-Maratier MM, Vandecasteele JP (1972) Consequence of lysine oversynthesis in Pseudomonas mutants insensitive to feedback inhibition. Eur J Biochem 30:100–106
James T, Jacobs M (1976) A p-fluorophenylalanine resistant mutant in Arabidopsis thaliana. In: Kranz AR (ed) Arabidopsis information service, Proc 2nd Int Symp Arabidopsis Res. Frankfurt, pp 85–95
Kueh JHS, Bright SWJ (1981) Proline accumulation in a barley mutant resistant to trans-4-hydroxy-L-proline. Planta 153:166–171
Kueh JHS, Bright SWJ (1982) Biochemical and genetical analysis of three proline accumulating barley mutants. Plant Sci Lett 27:233–241
Miflin BJ, Bright SWJ, Rognes SE, Kueh JHS (1983) Amino acids, nutrition and stress: the role of biochemical mutants in solving problems of crop quality. In: Kosuge T, Meredith CP, Hollaender A (eds) Genetic engineering of plants — an agricultural perspective. Plenum Press, London, pp 391–414
Mori S, Hasegawa H, Nakamura K, Nakanishi H, Murakami M (1985) Genetic analysis for hydroxyproline resistant mutants in rice (in Japanese). Jpn J Breed (Suppl) 35:150–151
Mori S, Hasegawa H, Nakamura K, Nakanishi H, Murakami M (1985) Characterization of callus-cell lines of rice resistant to hydroxy-L-proline. Rice Genet Newslett 2:89–91
Negrutiu I, Jacobs M, Cattoir A (1978) Arabidopsis thaliana L., espèce modele en génétique cellulaire. Physiol Veg 16:365–379
Shiomi N, Hori S (1973) Proline−14C metabolism in barley seedlings during vernalization. Plant Cell Physiol 14:1009–1018
Author information
Authors and Affiliations
Additional information
Communicated by F. Salamini
Rights and permissions
About this article
Cite this article
Hasegawa, H., Mori, S. Non-proline-accumulating rice mutants resistant to hydroxy-L-proline. Theoret. Appl. Genetics 72, 226–230 (1986). https://doi.org/10.1007/BF00266996
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00266996