Skip to main content
SpringerLink
Log in

Expression of epidermal growth factor in the rat kidney

An immunocytochemical and in situ hybridization study

  • Published:
Histochemistry Aims and scope Submit manuscript

Summary

The renal localization and the site of synthesis of epidermal growth factor (EGF) were investigated in the rat kidney by immunohistochemistry and in situ hybridization techniques. EGF was localized in the cells of the thick ascending limb of Henle (TAL) and distal convoluted tubule (DCT). At the ultrastructural level, EGF immunoreactivity was distributed on the apical membrane and trans-Golgi complex of the TAL and DCT cells. These segments of the rat nephron also hybridized to prepro-EGF cRNA probes in a specific manner, indicating that TAL and DCT are the sites of EGF synthesis in the rat kidney.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adler S, Eng B (1990) Reversal of inhibition of rat glomerular epithelial cell growth by growth factors. Am J Pathol 136:557–563

    Google Scholar 

  • Atkin BM, Franklin WA, Bell GI, Toback FG (1990) Macula densa of mouse kidney do not synthesize epidermal growth factor precursor mRNA. Nephron 54:313–317

    Google Scholar 

  • Bell GI, Fong NM, Stempien MM, Worsted MA, Capt D, Ku L, Uredea MS, Rall LB, Sanchez-Pescador R (1986) Human epidermal growth factor precursor: cDNA sequence, expression in vivo and gene organization. Nucleic Acids Res 14:8427–8446

    Google Scholar 

  • Boventre JV, Gronich JH, Nemenoff RA (1990) Epidermal growth factor enhances glomerular mesangial cell soluble phospholipase A2 activity. J Biol Chem 265:4934–4938

    Google Scholar 

  • Breyer JA, Cohen S (1990) The epidermal growth factor precursor isolated from murine kidney membranes. J Biol Chem 265:16564–16570

    Google Scholar 

  • Breyer MD, Jacobson HR, Breyer J (1988) Epidermal growth factor inhibits both chlorophenylthio-cyclic AMP and vasopressin stimulated hydraulic conductivity in the rabbit cortical collecting tubule. J Clin Invest 82:1313–1320

    Google Scholar 

  • Carpenter G, Cohen S (1979) Epidermal growth factor. Annu Rev Biochem 48:193–216

    Google Scholar 

  • Dorow DS, Simpson RJ (1988) Cloning and sequence analysis of a cDNA for rat epidermal growth factor. Nucl Acid Res 16:9338

    Google Scholar 

  • Fisher DA, Lakshmanan J (1990) Metabolism and effects of epidermal growth factor and related growth factors in mammals. Endocr Rev 11:418–442

    Google Scholar 

  • Fisher DA, Salido EC, Barajas L (1989) Epidermal growth factor and the kidney. Annu Rev Physiol 51:67–80

    Google Scholar 

  • Goodyer PR, Kachra Z, Bell C, Rozen R (1988) Renal tubular cells are potential targets for epidermal growth factor. Am J Physiol 255:F1191-F1196

    Google Scholar 

  • Gresik EW, Gubits RM, Barka T (1985) In situ localization of mRNA for epidermal growth factor in the submandibular gland of the mouse. J Histochem Cytochem 33:1235–1240

    Google Scholar 

  • Gusterson B, Cowley G, Smith JA, Ozanne B (1984) Cellular localization of human epidermal growth factor receptor. Cell Biol Int Rep 8:649–658

    Google Scholar 

  • Harris RC (1989) Response of rat inner medullary collecting duct to epidermal growth factor. Am J Physiol 256:F1117-F11124

    Google Scholar 

  • Harris RC, Daniel TO (1989) Epidermal growth factor binding, stimulation of phosphorylation, and inhibition of gluconeogenesis in rat proximal tubule. J Cell Physiol 139:383–391

    Google Scholar 

  • Harris RC, Hoover RL, Jacobson HR, Badr KF (1988) Evidence for glomerular actions of epidermal growth factor in the rat. J Clin Invest 82:1028–1039

    Google Scholar 

  • Hirata Y, Orth DN (1979a) Concentrations of epidermal growth factor, nerve growth factor and submandibular gland renin in male and female mouse tissues and fluids. Endocrinology 105:1382–1387

    Google Scholar 

  • Hirata Y, Orth DN (1979b) Epidermal growth factor (urogastrone) in human tissues. J Clin Endocrinol Metab 48:667–672

    Google Scholar 

  • Humes DH, Cieslinski DA, Coimbra, Messana JM, Galvao C (1989) Epidermal growth factor enhances renal tubule cell regeneration and repair and accelerates the recovery of renal function in postischemic acute renal failure. J Clin Invest 84:1757–1761

    Google Scholar 

  • Kasselberg AG, Orth DN, Gray ME, Stahlman MT (1985) Immunohistochemical localization of human epidermal growth factor/urogastrone in several human tissues. J Histochem Cytochem 33:315–322

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  • Margolis BL, Bonventre JV, Kremer SG, Kudlow JE, Skorecki KL (1988) Epidermal growth factor is synergistic with phorbol esters and vasopressin in stimulating arachidonate release and prostaglandin production in renal glomerular mesangial cells. Biochem J 249:587–592

    Google Scholar 

  • Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucl Acids Res 12:7035–7056

    Google Scholar 

  • Moore JB Jr (1978) Purification and partial characterization of epidermal growth factor from male rat submaxillary gland. Arch Biochem Biophys 189:1–7

    Google Scholar 

  • Mroczkowski B, Reich M, Chen K, Bell GI, Cohen S (1989) Recombinant human epidermal growth factor precursor is a glycosylated membrane protein with biological activity. Mol Cell Biol 9:2771–2778

    Google Scholar 

  • Norman J, Badie-Dezfooly B, Nord EP, Kurtz I, Schlosser J, Chaudhari A, Fine LG (1987) EGF-induced mitogenesis in proximal tubular cells; potentiation by angiotensin II. Am J Physiol 253:F299-F309

    Google Scholar 

  • Olsen PS, Nexo E, Poulsen SS, Hansen F, Kirkegaard P (1984) Renal origin of rat urinary epidermal growth factor. Regul Rept 10:37–45

    Google Scholar 

  • Poulsen SS, Nexo E, Olsen S, Hess J, Kirkegaard P (1986) Immunohistochemical localization of epidermal growth factor in rat and man. Histochemistry 85:389–394

    Google Scholar 

  • Rall LB, Scott J, Bell GI, Crawford RJ, Penshow JD, Niall HD, Coghlan JP (1985) Mouse prepro-epidermal growth factor synthesis by the kidney and other tissues. Nature 313:228–231

    Google Scholar 

  • Real FX, Rettig WJ, Chesa PG, Melamed MR, Old LJ, Mendelsohn J (1986) Expression of epidermal growth factor receptor in human cultured cells and tissues: relationship to cell lineage and stage of differentiation. Cancer Res 46:4726–4731

    Google Scholar 

  • Rubin JS, Bradshaw RA (1984) Preparation of guinea pig prostate epidermal growth factor. In: Methods for preparation of media, supplements, and substrata for serum-free animal cell culture. In: Barnes DW, Sirhbasku DA, Sato GH (eds) Culture methods for molecular and cell biology. Liss, New York, pp 139–145

    Google Scholar 

  • Safirstein R, Zelent AZ, Price PM (1989) Reduced renal preproepidermal growth factor mRNA and decreased EGF excretion in ARF. Kidney Int 36:810–815

    Google Scholar 

  • Salido EC, Fisher DA, Barajas L (1986) Immunoelectron microscopy of epidermal growth factor in mouse kidney. J Ultrastruct Mol Struct Res 96:105–113

    Google Scholar 

  • Salido EC, Yen PH, Shapiro LJ, Fisher DA, Barajas L (1989) In situ hybridization of prepro-epidermal growth factor mRNA in the mouse kidney. Am J Physiol 256:F632-F638

    Google Scholar 

  • Schaudies RP, Savage CR Jr (1986) Isolation of rat epidermal growth factor (rEGF): chemical, biochemical and immunological comparisons with mouse and human EGF. Comp Biochem Physiol 84B:497–505

    Google Scholar 

  • Scott J, Urdea M, Quiroga M, Sanchez-Pescador R, Fong N, Selby M, Rutter WJ, Bell GI (1983) Structure of a mouse submaxillary messenger RNA encoding epidermal growth factor and seven related proteins. Science 221:236–240

    Google Scholar 

  • Simpson RJ, Smith JA, Moritz RL, O'Hare MJ, Rudland PS, Morrison JR, Lloyd CJ, Grego B, Burgess AW, Nice EC (1985) Rat epidermal growth factor: complete amino acid sequence. Homology with the corresponding murine and human proteins; isolation of a form truncated at both ends with full in vitro biological activity. Eur J Biochem 153:629–637

    Google Scholar 

  • Stanton RC, Seifter JL (1988) Epidermal growth factor rapidly activates the hexose monophosphate shunt in kidney cells. Am J Physiol 253:C267-C271

    Google Scholar 

  • Vehaskari VM, Hering-Smith KS, Moskowitz DW, Weiner ID, Hamm LL (1989) Effect of epidermal growth factor on sodium transport in the cortical collecting tubule. Am J Physiol 256:F803-F809

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, School of Medicine and the Howard Hughes Medical Institute, University of California, Los Angeles, Harbor-UCLA Medical Center, 1000 West Carson Street, 90509, Torrance, CA, USA

    E. C. Salido & L. Barajas

  2. Department of Pediatrics, School of Medicine and the Howard Hughes Medical Institute, University of California, Los Angeles, Harbor-UCLA Medical Center, 1000 West Carson Street, 90509, Torrance, CA, USA

    J. Lakshmanan & D. A. Fisher

  3. Department of Biological Chemistry, School of Medicine and the Howard Hughes Medical Institute, University of California, Los Angeles, Harbor-UCLA Medical Center, 1000 West Carson Street, 90509, Torrance, CA, USA

    L. J. Shapiro

Authors
  1. E. C. Salido
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Lakshmanan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. A. Fisher
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. J. Shapiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. L. Barajas
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Salido, E.C., Lakshmanan, J., Fisher, D.A. et al. Expression of epidermal growth factor in the rat kidney. Histochemistry 96, 65–72 (1991). https://doi.org/10.1007/BF00266763

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00266763

Keywords

Search

Navigation