Summary
The mating type gene MA TA of the dimorphic yeast Yarrowia lipolytica was cloned. The strategy used was based on the presumed function of this gene in the induction of sporulation. A diploid strain homozygous for the mating type B was transformed with an integrative gene bank from an A wild-type strain. A sporulating transformant was isolated, which contained a plasmid with an 11.6 kb insert. This sequence was rescued from the chromosomal DNA of the transformant and deletion mapping was performed to localize the MAT insert. The MAT gene conferred both sporulating and non-mating phenotypes on a B/B diploid. A LEU2 sequence targeted to this locus segregated like a mating type-linked gene. The A strain did not contain silent copies of the MAT gene.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barth G, Weber H (1985) Improvement of sporulation in the yeast Yarrowia lipolytica. Antonie van Leeuwenhoek 51:167–177
Davidow LS, Apostolakos D, O'Donnell MM, Proctor AR, Ogrydziak DM, Wing RA, Stasko I, DeZeeuw JR (1985) Integrative transformation of the yeast Yarrowia lipolytica. Curr Genet 10:39–48
Gaillardin C, Ribet A-M (1987) LEU2 directed expression of β-galactosidase activity and phleomycin resistance in Yarrowia lipolytica. Curt Genet 11:369–375
Giasson L, Specht CA, Milgrim C, Novotny CP, Ullrich RC (1989) Cloning and comparison of Aa mating-type alleles of the basidiomycete Schizophyllum. Mol Gen Genet 218:72–77
Glass NL, Vollmer SJ, Staben C, Grotelueschen J, Metzenberg RL, Yanofsky C (1988) DNAs of the two mating-type alleles of Neurospora crassa are highly dissimilar. Science 241:570–573
Herskowitz I (1989) A regulatory hierarchy for cell specialization in yeast. Nature 342:749–757
Hicks J, Strathern JN, Klar AJS (1979) Transposable mating type genes in S. cerevisiae. Nature 282:478–483
Kelly M, Burke J, Smith M, Klar A, Beach D (1988) Four matingtype genes control sexual differentiation in the fission yeast. EMBO J 7:1537–1547
Kurischko C, Inge-Vechtomov SG, Weber H (1983) Development of breeding stocks of the yeast Saccharomycopsis lipolytica by methods of moderate inbreeding. Z Allg Mikrobiol 23:513–515
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Mutasa ES, Tymon AM, Göttgens B, Mellon FM, Little PFR, Casselton LA (1990) Molecular organization of an A mating type factor of the basidiomycete fungus Coprinus cinereus. Curr Genet 18:223–229
Nasmyth KA, Tatchell K (1980) The structure of transposable yeast mating type loci. Cell 19:753–764
Ogrydziak D, Bassel J, Contopoulou R, Mortimer R (1978) Development of genetic techniques and the genetic map of the yeast Saccharomycopsis lipolytica. Mol Gen Genet 163:229–239
Picard M, Debuchy R, Coppin E (1991) Cloning the mating types of the heterothallic fungus Podospora anserina: developmental features of haploid transformants carrying both mating types. Genetics 128:539–547
Schulz B, Banuett F, Dahl M, Schlesinger R, Schäfer W, Martin T, Herskowitz I, Kahmann R (1990) The b alleles of U. maydis, whose combinations program pathogenic development, code for polypeptides containing a homeodomain-related motif. Cell 60:295–306
Sprague GF, Rine J, Herskowitz I (1981) Homology and nonhomology at the yeast mating type locus. Nature 289:250–252
Stahl U (1978) Zygote formation and recombination between like mating types in the yeast Saccharomycopsis lipolytica by protoplast fusion. Mol Gen Genet 160:111–113
Van der Walt JP, Von Arx JA (1980) The yeast genus Yarrowia gen. nov. Antonie van Leeuwenhoek 46:517–521
Weber H, Kurischko C (1989) Sexual behavior in the alkane-utilizing yeast Yarrowia lipolytica. Yeast 5 (Special Issue): 279–285
Weber H, Spata L (1981) Characterization of yeast protoplastfusion products. In: Stewart GG, Russel I (eds) Current developments in yeast research. Advances in biotechnology: Proceedings of the 6th International Fermentation Symposium 5th International Symposium on Yeasts. Pergamon Press, London, Canada, pp 213–218
Weber H, Kurischko C, Barth G (1988) Mating in the alkane-utilizing yeast Yarrowia lipolytica. J Basic Microbiol 28:229–240
Wickerham LJ (1951) Taxonomy of yeast. Tech Bull US Dept Agric Washington 1029:1–56
Wickerham LJ, Kurtzman CP, Herman AI (1970) Sexuality in Candida lipolytica. In: Ahearn DG (ed) Recent trends in yeast research, Georgia State University, Atlanta GA, pp 81–92
Xuan JW, Fournier P, Gaillardin C (1988) Cloning of the LYS5 gene encoding saccharopine dehydrogenase from the yeast Y. lipolytica. Curr Genet 14:19–21
Author information
Authors and Affiliations
Additional information
Communicated by C.P. Hollenberg
Rights and permissions
About this article
Cite this article
Kurischko, C., Fournier, P., Chasles, M. et al. Cloning of the mating-type gene MATA of the yeast Yarrowia lipolytica . Molec. Gen. Genet. 232, 423–426 (1992). https://doi.org/10.1007/BF00266246
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00266246