Summary
A considerable amount of variation with respect to soluble proteins and esterase isoenzyme pattern was observed between different species of Brassica. Naturally occurring amphidiploids had comparable proteins and isoenzyme patterns to either one or both of the parental species. The species relationship based on percentage homology of protein and esterase bands revealed that B. nigra and B. campestris are the parental species of B. carinata and not B. nigra and B. oleracea, as suggested on the basis of cytological studies. Elimination of a pair of chromosomes might have resulted into 2n=34 in the case of B. carinata. Further studies are needed to confirm this view. The peroxidase and catalase isoenzyme patterns did not show much variation in different species and amphidiploids.
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Literature
Cherry, J.P.; Katterman, F.R.H.; Endrizzi, J.E.: Comparative studies of seed proteins of species of Gossypium by gel electrophoresis. Evolution 24, 431–447 (1970)
Cherry, J.P.; Katterman, F.R.H.; Endrizzi, J.E.: A comparative study of seed proteins of allopolyploids of Gossypium by gel electrophoresis. Canad. J. Genet. Cytol. 13, 155–158 (1971)
Davis, B.J.: Disc electrophoresis. II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121, 407–427 (1964)
Desborough, S.L.; Peloquin, S.J.: Disc electrophoresis of tuber proteins from Solanum species and interspecific hybrids. Phytochemistry 5, 727–733 (1966)
Gardiner, M.G.; Cleland, R.: Peroxidase isozymes in Avena coleoptile. Phytochemistry 13, 1707–1711 (1974)
Hart, G.E.; Bhatia, C.R.: Acrylamide gel electrophoresis of soluble leaf proteins and enzymes from Nicotiana species. Canad. J. Genet. Cytol. 9, 367–374 (1967)
Johnson, B.L.; Hall, D.: Analysis of phylogenetic affinities in the Triticinae by protein electrophoresis. Amer. J. Bot. 52, 506–513 (1965)
Ladizinski, G.; Johnson, B.L.: Seed protein homologies and the evolution of polyploidy in Avena. Canad. J. Genet. Cytol. 14, 875–888 (1972)
Lodha, M.L.; Mali, P.C.; Aggarwal, A.K.; Mehta, S.L.: Changes in soluble proteins and isoenzymes in normal and opaque-2 Zea mays endosperm during grain development. Phytochemistry 13, 539–542 (1974)
Lowry, O.M.; Rosebrough, N.J.; Farr, A.L.; Randall, R.J.: Protein measurement with folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
Mitra, R.; Jagannath, D.R.; Bhatia, C.R.: Disc electrophoresis of analogous enzymes in Hordeum. Phytochemistry 9, 1843–1850 (1970)
Murray, B.E.; Craig, I.L.; Rajhathy, T.: A protein electrophoretic study of three amphidiploids and eight species of Avena. Canad. J. Genet. Cytol. 12, 651–655 (1970)
Nainawatee, H.S.; Das, N.B.: Species relationship in Triticum by protein electrophoresis. Curr. Sci. 41, 778–779 (1972)
Reddy, M.M.; Garber, E.D.: Genetic studies of varient enzymes. III Comparitive electrophoretic studies of esterases and peroxidases for species, hybrids and amphidiploids in the genus Nicotiana. Bot. Gaz. 132, 158–166 (1971)
Scandalios, J.G.: Genetic control of multiple forms of enzymes in plants. A Review. Biochem. Genet. 3, 37–79 (1969)
Sheen, S.J.: Isozyme evidence bearing on the origin of Nicotiana tabacum L. Evolution 26, 143–154 (1972)
Siddiqi, E.A.; Nerkar, Y.S.; Mehta, S.L.: Intra and interspecific variation in soluble proteins of Oryza sativa L. Theor. Appl. Genet. 42, 351–356 (1972)
Vaughan, J.G.: A multidisciplinary study of the taxonomy and origin of Brassica crops. Bioscience 27, 35–40 (1977)
Waines, G.J.; Johnson, B.L.: Incomplete concurrence of evidence from protein electrophoresis and genome analysis regarding the origin of A. ovata. Canad. J. Genet. Cytol. 17, 1–8 (1975)
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Communicated by B.R. Murty
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Yadava, J.S., Chowdhury, J.B., Kakar, S.N. et al. Comparative electrophoretic studies of proteins and enzymes of some Brassica species. Theoret. Appl. Genetics 54, 89–91 (1979). https://doi.org/10.1007/BF00265475
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DOI: https://doi.org/10.1007/BF00265475