Summary
Mutant cell lines lacking nitrate reductase activity were analyzed genetically. Protoplasts from one apoprotein defective (nia) and four cofactor defective (cnx) mutants were fused in all possible pairwise combinations with the aid of polyethylene glycol. Complementing hybrids were detected by their ability to grow with nitrate as sole nitrogen source and confirmed by measuring their nitrate reductase activity. Strong complementation was observed in all types of nia+cnx hybrids, whereas the cnx mutants failed to complement each other. From the results it can be concluded that the mutants studied are recessive and that the four cnx mutants are alleles of the same pair of duplicate loci (cnxA1, cnxA2).
Similar content being viewed by others
References
Cove DJ (1976) Chlorate toxicity in Aspergillus nidulans: the selection and characterization of chlorate resistant mutants. Heredity 36:191–203
Cove DJ (1979) Genetic studies of nitrate assimilation in Aspergillus nidulans. Biol Rev Cambridge Philos Soc 54:291–327
Glimelius K, Eriksson T, Grafe R, Müller AJ (1978) Somatic hybridization of nitrate reductase-deficient mutants of Nicotiana tabacum by protoplast fusion. Physiol Plant 44:273–277
Mendel RR, Müller AJ (1979) Nitrate reductase-deficient mutant cell lines of Nicotiana tabacum. Further biochemical characterization. Mol Gen Genet 177:145–153
Mendel RR, Alikulov ZA, Lvov NP, Müller AJ (1981) Presence of the molybdenum-cofactor in nitrate reductase-deficient mutant cell lines of Nicotiana tabacum. Mol Gen Genet 181:395–399
Mendel RR, Alikulov ZA, Müller AJ (1982) Molybdenum cofactor in nitrate reductase-deficient tobacco mutants. 3. Induction of cofactor synthesis by nitrate. Plant Sci Lett 27:95–101
Marton L, Dung TM, Mendel RR, Maliga P (1982) Nitrate reductase deficient cell lines from haploid protoplast cultures of Nicotiana plumbaginifolia. Mol Gen Genet 182:301–304
Müller AJ, Grafe R (1978) Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol Gen Genet 161:67–76
Author information
Authors and Affiliations
Additional information
Communicated by R. Hagemann
Rights and permissions
About this article
Cite this article
Grafe, R., Müller, A.J. Complementation analysis of nitrate reductase deficient mutants of Nicotiana tabacum by somatic hybridization. Theoret. Appl. Genetics 66, 127–130 (1983). https://doi.org/10.1007/BF00265186
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00265186